Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,439,414 C→G 16.2% L296L (CTG→CTC) ‡ ompC ← outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,439,4140CG16.2% 332.7 / 45.3 130L296L (CTG→CTC) ‡ompCouter membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base C (46/62);  new base G (9/12);  total (56/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.52e-01

Only 100 of 135 total aligned reads displayed.

AACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCC  >  Exported/1439345‑1439483
                                                                     |                                                                     
aacatATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGcc                                                                       <  1:5253107/70‑1 (MQ=255)
 acatATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAg                                                                     >  1:2265582/1‑71 (MQ=255)
 acatATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAg                                                                     >  1:2539654/1‑71 (MQ=255)
 acatATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAg                                                                     >  1:222512/1‑71 (MQ=255)
   atatTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAgg                                                                    >  1:192306/1‑70 (MQ=255)
   atatTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAgg                                                                    >  1:1765487/1‑70 (MQ=255)
      tttCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacg                                                                <  1:1095544/71‑1 (MQ=255)
      tttCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacg                                                                >  1:668731/1‑71 (MQ=255)
      tttCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacg                                                                >  1:3325632/1‑71 (MQ=255)
      tttCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacg                                                                <  1:4508043/71‑1 (MQ=255)
      tttCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggac                                                                 <  1:4979120/70‑1 (MQ=255)
       ttCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacg                                                                >  1:3540881/1‑70 (MQ=255)
        tCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAgg                                                                    <  1:3578828/65‑1 (MQ=255)
        tCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAgg                                                                    <  1:1319975/65‑1 (MQ=255)
          aGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGgacggacg                                                            <  1:747491/71‑1 (MQ=255)
            gATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCa                                                          <  1:1458373/71‑1 (MQ=255)
              tatCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCa                                                          <  1:4449484/69‑1 (MQ=255)
              tatCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGa                                                        <  1:2167186/71‑1 (MQ=255)
               atCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGAc                                                       >  1:4839122/1‑71 (MQ=255)
                  ttCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg                                                     >  1:2203836/1‑70 (MQ=255)
                   tcgtcgtcgTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacgga                                                              >  1:964194/1‑60 (MQ=255)
                    cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg                                                     <  1:4221174/68‑1 (MQ=255)
                    cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg                                                     <  1:1738365/68‑1 (MQ=255)
                    cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg                                                     <  1:2602749/68‑1 (MQ=255)
                    cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg                                                     <  1:4553075/68‑1 (MQ=255)
                    cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGaa                                                   <  1:2506492/70‑1 (MQ=255)
                    cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGa                                                    <  1:4591558/69‑1 (MQ=255)
                    cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAg                                                  >  1:1134832/1‑71 (MQ=255)
                     gtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGt                                                 >  1:3647463/1‑71 (MQ=255)
                      tcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGcc                                                                       <  1:1669508/48‑1 (MQ=255)
                          cgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAAc                                            >  1:2020717/1‑71 (MQ=255)
                           gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAAc                                            <  1:1064020/70‑1 (MQ=255)
                           gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACt                                           >  1:4348782/1‑71 (MQ=255)
                           gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGAAGACCGAAGTCGAAc                                            <  1:3198147/70‑1 (MQ=255)
                              gCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTgg                                         <  1:5365246/70‑1 (MQ=255)
                              gCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTgg                                         <  1:5136905/70‑1 (MQ=255)
                              gCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTgg                                         <  1:4766235/70‑1 (MQ=255)
                               ccACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAgg                                                                    <  1:4403093/42‑1 (MQ=255)
                                    aCCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAg                                                                     >  1:1614130/1‑36 (MQ=255)
                                      ccAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacg                                                                <  1:4215898/39‑1 (MQ=255)
                                        aGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacgga                                                              >  1:3386334/1‑39 (MQ=255)
                                        aGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAAc                              <  1:5095140/71‑1 (MQ=255)
                                         ggTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGaa                                             >  1:5534720/1‑55 (MQ=255)
                                           tttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGaa                                                   <  1:2070705/47‑1 (MQ=255)
                                            ttttACCTTTAGACTGCAGGTAAGCCAGGgacggacg                                                            <  1:2234658/37‑1 (MQ=255)
                                            ttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCg                                               >  1:104976/1‑50 (MQ=255)
                                            ttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCg                                               >  1:3046131/1‑50 (MQ=255)
                                            ttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCt                          >  1:5083192/1‑71 (MQ=255)
                                            ttttACCTTTAGACTGCAGGTAAGCCAGCGACGGACGCAGACCGAAGTCg                                               >  1:282336/1‑50 (MQ=255)
                                             tttACCTTTAGACTGCAGGTAAGCCAGGgacggacg                                                            <  1:957795/36‑1 (MQ=255)
                                             tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGaa                                             >  1:5096822/1‑51 (MQ=255)
                                             tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGc                                 <  1:5545305/63‑1 (MQ=255)
                                             tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCtt                         >  1:1710827/1‑71 (MQ=255)
                                             tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCtt                         >  1:359277/1‑71 (MQ=255)
                                             tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCtt                         >  1:3856771/1‑71 (MQ=255)
                                             tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCt                          <  1:1406890/70‑1 (MQ=255)
                                             cttACCTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACt                                           >  1:3008237/2‑53 (MQ=12)
                                              ttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGt                                                 >  1:2741014/1‑46 (MQ=255)
                                               tACCTTTAGATTGCAGGTAAGAGATGGACGGGCGCAGGCCAAAGTCGAACTGATACt                                     >  1:2668514/1‑57 (MQ=255)
                                               tACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCaa                               >  1:5181823/1‑63 (MQ=255)
                                               tACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTc                        <  1:4635071/70‑1 (MQ=255)
                                                aCCTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTca                       <  1:1537705/70‑2 (MQ=18)
                                                aCCTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTca                       <  1:2125179/70‑2 (MQ=18)
                                                aCCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg                       <  1:229860/70‑1 (MQ=255)
                                                aCCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg                       <  1:492745/70‑1 (MQ=255)
                                                aCCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg                       >  1:3167355/1‑70 (MQ=255)
                                                aCCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg                       <  1:2820980/70‑1 (MQ=255)
                                                 ccTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTca                       <  1:2128302/69‑2 (MQ=18)
                                                  cTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAg                    >  1:1664479/1‑71 (MQ=255)
                                                  cTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAg                    >  1:4586901/1‑71 (MQ=255)
                                                    ttAGATTGCAGGTAAGCGATGGCCGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGtt                  >  1:2825957/1‑71 (MQ=16)
                                                    ttAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGATGTCGAACTGGTACTGAGCAACAGCTTCGAAGtt                  >  1:2920638/1‑71 (MQ=255)
                                                    ttAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGtt                  >  1:1420880/1‑71 (MQ=255)
                                                    ttAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGt                   >  1:640514/1‑70 (MQ=255)
                                                     tAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGt                   <  1:1950475/69‑1 (MQ=12)
                                                     tAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGtt                  <  1:4315465/70‑1 (MQ=255)
                                                      aGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATa                                       >  1:2729144/1‑48 (MQ=2)
                                                      aGATTGCAGGTAAGCAACGGACGGACGCAGACCGAAATCGAACTGATATTGTGCAACAGCTTCGAAGtttt                >  1:4744142/1‑69 (MQ=17)
                                                      aGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTc                 <  1:3576373/70‑1 (MQ=255)
                                                       gACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCt                <  1:2782426/70‑1 (MQ=255)
                                                         cTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtgtg             <  1:839806/71‑1 (MQ=255)
                                                            cAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAAca                                           >  1:1757761/1‑37 (MQ=255)
                                                            cAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCtt          <  1:3774929/71‑1 (MQ=255)
                                                               gTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTc                        <  1:1866340/54‑1 (MQ=9)
                                                               gTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGt       >  1:5621379/1‑71 (MQ=12)
                                                               gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACt                                           <  1:305950/35‑1 (MQ=255)
                                                               gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACt                                           <  1:5455682/35‑1 (MQ=255)
                                                               gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACt                                           <  1:1653707/35‑1 (MQ=255)
                                                               gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtg               <  1:5203468/63‑1 (MQ=255)
                                                               gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtg               <  1:4573596/63‑1 (MQ=255)
                                                                tAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGtt      <  1:5201831/71‑1 (MQ=12)
                                                                tAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGtt      <  1:212433/71‑1 (MQ=12)
                                                                tAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGtt      <  1:1279895/71‑1 (MQ=12)
                                                                tAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGtt      <  1:4205124/71‑1 (MQ=255)
                                                                 aaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACa                             >  1:741568/1‑47 (MQ=255)
                                                                 aaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGc                           >  1:3181866/1‑49 (MQ=255)
                                                                  aGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCg    >  1:3673739/1‑71 (MQ=255)
                                                                    ccAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTccca  <  1:4463286/71‑4 (MQ=255)
                                                                     cAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGcc  >  1:3970357/1‑70 (MQ=255)
                                                                     cAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGcc  >  1:4290362/1‑70 (MQ=255)
                                                                     |                                                                     
AACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCC  >  Exported/1439345‑1439483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: