Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,439,416 G→T 17.3% L296M (CTG→ATG) ‡ ompC ← outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,439,4160GT17.3% 344.9 / 36.2 122L296M (CTG→ATG) ‡ompCouter membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base G (41/59);  new base T (9/12);  total (51/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 137 total aligned reads displayed.

ACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCA  >  Exported/1439346‑1439485
                                                                      |                                                                     
     tttCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacg                                                                  <  1:4508043/71‑1 (MQ=255)
     tttCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacg                                                                  >  1:3325632/1‑71 (MQ=255)
     tttCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacg                                                                  >  1:668731/1‑71 (MQ=255)
     tttCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggac                                                                   <  1:4286671/70‑1 (MQ=255)
     tttCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggac                                                                   <  1:4979120/70‑1 (MQ=255)
      ttCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacg                                                                  >  1:3540881/1‑70 (MQ=255)
       tCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAgg                                                                      <  1:1319975/65‑1 (MQ=255)
       tCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAgg                                                                      <  1:3578828/65‑1 (MQ=255)
         aGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGgacggacg                                                              <  1:747491/71‑1 (MQ=255)
         aGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGgacggacg                                                              <  1:2320555/71‑1 (MQ=255)
           gATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCa                                                            <  1:542678/71‑1 (MQ=255)
           gATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCa                                                            <  1:1458373/71‑1 (MQ=255)
             tatCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCa                                                            <  1:4449484/69‑1 (MQ=255)
             tatCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGa                                                          <  1:2167186/71‑1 (MQ=255)
              atCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGAc                                                         >  1:4839122/1‑71 (MQ=255)
                 ttCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGCCCg                                                       >  1:513285/1‑70 (MQ=255)
                 ttCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg                                                       >  1:2203836/1‑70 (MQ=255)
                  tcgtcgtcgTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacgga                                                                >  1:964194/1‑60 (MQ=255)
                   cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg                                                       <  1:4553075/68‑1 (MQ=255)
                   cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg                                                       <  1:2602749/68‑1 (MQ=255)
                   cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg                                                       <  1:1738365/68‑1 (MQ=255)
                   cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGaa                                                     <  1:2506492/70‑1 (MQ=255)
                   cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAg                                                    >  1:1134832/1‑71 (MQ=255)
                    gtcgtcgtAGCCACGACCCAGGTTTTTACCTTTGGACTGCAGGTAAGCCAGGGACGGACGCAGACCGaa                                                     <  1:2625254/69‑1 (MQ=255)
                    gtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGt                                                   >  1:3647463/1‑71 (MQ=255)
                       gtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGa                                                <  1:1833562/71‑1 (MQ=255)
                       gtcgtAGCAACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAg                                                                       >  1:1661947/1‑48 (MQ=255)
                         cgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAAc                                              >  1:2020717/1‑71 (MQ=255)
                          gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAAc                                              <  1:4714787/70‑1 (MQ=255)
                          gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAAc                                              <  1:1064020/70‑1 (MQ=255)
                          gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACt                                             >  1:4348782/1‑71 (MQ=255)
                          gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGAAGACCGAAGTCGAAc                                              <  1:3198147/70‑1 (MQ=255)
                             gCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTgg                                           <  1:4766235/70‑1 (MQ=255)
                             gCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTgg                                           <  1:5365246/70‑1 (MQ=255)
                             gCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTgg                                           <  1:2769725/70‑1 (MQ=255)
                              ccACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAgg                                                                      <  1:4403093/42‑1 (MQ=255)
                                aCGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACt                                       >  1:4915708/1‑71 (MQ=255)
                                     ccAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacg                                                                  <  1:4215898/39‑1 (MQ=255)
                                       aGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacgga                                                                >  1:3386334/1‑39 (MQ=255)
                                           ttttACCTTTAGACTGCAGGTAAGCCAGGgacggacg                                                              <  1:2234658/37‑1 (MQ=255)
                                           ttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCg                                                 >  1:3046131/1‑50 (MQ=255)
                                           ttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCg                                                 >  1:104976/1‑50 (MQ=255)
                                           ttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCt                            >  1:5083192/1‑71 (MQ=255)
                                            tttACCTTTAGACTGCAGGTAAGCCAGGgacggacg                                                              <  1:957795/36‑1 (MQ=255)
                                            tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGaa                                               >  1:5096822/1‑51 (MQ=255)
                                            tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGc                                   <  1:5545305/63‑1 (MQ=255)
                                            tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCtt                           >  1:3856771/1‑71 (MQ=255)
                                            tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCtt                           >  1:359277/1‑71 (MQ=255)
                                            tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCt                            <  1:1406890/70‑1 (MQ=255)
                                            tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCt                            <  1:2948643/70‑1 (MQ=255)
                                             ttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGt                                                   >  1:2741014/1‑46 (MQ=255)
                                              tACCTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACt                                       >  1:4142245/1‑57 (MQ=12)
                                              tACCTTTAGATTGCAGGTAAGAGATGGACGGGCGCAGGCCAAAGTCGAACTGATACt                                       >  1:2668514/1‑57 (MQ=255)
                                              tACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCaa                                 >  1:5181823/1‑63 (MQ=255)
                                              tACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTc                          <  1:4635071/70‑1 (MQ=255)
                                               aCCTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTca                         <  1:2125179/70‑2 (MQ=18)
                                               aCCTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTca                         <  1:1537705/70‑2 (MQ=18)
                                               aCCTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTc                          >  1:207291/1‑69 (MQ=18)
                                               aCCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg                         <  1:2820980/70‑1 (MQ=255)
                                               aCCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg                         <  1:229860/70‑1 (MQ=255)
                                               aCCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg                         >  1:3167355/1‑70 (MQ=255)
                                                 cTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAg                      >  1:1664479/1‑71 (MQ=255)
                                                 cTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAg                      >  1:4586901/1‑71 (MQ=255)
                                                   ttAGATTGCAGGTAAGCGATGGCCGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGtt                    >  1:2825957/1‑71 (MQ=16)
                                                   ttAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGATGTCGAACTGGTACTGAGCAACAGCTTCGAAGtt                    >  1:2920638/1‑71 (MQ=255)
                                                   ttAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGtt                    >  1:1420880/1‑71 (MQ=255)
                                                   ttAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGt                     >  1:640514/1‑70 (MQ=255)
                                                    tAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGtt                    <  1:3856330/70‑1 (MQ=16)
                                                    tAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGtt                    <  1:4497202/70‑1 (MQ=16)
                                                    tAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGt                     <  1:1950475/69‑1 (MQ=12)
                                                     aGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATa                                         >  1:2225626/1‑48 (MQ=2)
                                                     aGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGttt                   <  1:1728506/70‑2 (MQ=12)
                                                     aGATTGCAGGTAAGCAACGGACGGACGCAGACCGAAATCGAACTGATATTGTGCAACAGCTTCGAAGtttt                  >  1:4744142/1‑69 (MQ=17)
                                                     aGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTc                   <  1:3576373/70‑1 (MQ=255)
                                                        cTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtgtg               <  1:839806/71‑1 (MQ=255)
                                                           cAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAAca                                             >  1:1757761/1‑37 (MQ=255)
                                                           cAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCtt            <  1:3774929/71‑1 (MQ=255)
                                                             ggTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCt             <  1:1927208/68‑1 (MQ=255)
                                                             ggTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg          <  1:5580003/71‑1 (MQ=255)
                                                              gTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTc                          <  1:1866340/54‑1 (MQ=9)
                                                              gTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGt         >  1:5621379/1‑71 (MQ=12)
                                                              gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACt                                             <  1:1653707/35‑1 (MQ=255)
                                                              gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACt                                             <  1:5455682/35‑1 (MQ=255)
                                                              gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACt                                             <  1:305950/35‑1 (MQ=255)
                                                              gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtg                 <  1:4577851/63‑1 (MQ=255)
                                                               tAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGtt        <  1:212433/71‑1 (MQ=12)
                                                               tAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGtt        <  1:5201831/71‑1 (MQ=12)
                                                               tAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGtt        <  1:1279895/71‑1 (MQ=12)
                                                               tAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtg                 <  1:5024250/62‑1 (MQ=255)
                                                               tAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGtt        <  1:4205124/71‑1 (MQ=255)
                                                                aaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACa                               >  1:741568/1‑47 (MQ=255)
                                                                aaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGc                             >  1:3181866/1‑49 (MQ=255)
                                                                aaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTc       >  1:2821390/1‑71 (MQ=255)
                                                                aaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTc       >  1:2164181/1‑71 (MQ=255)
                                                                 aGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCg      >  1:5202142/1‑71 (MQ=255)
                                                                 aGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCg      >  1:3673739/1‑71 (MQ=255)
                                                                    cAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGcc    >  1:4290362/1‑70 (MQ=255)
                                                                      gggACGGACGCAGACCGAAGTCTAACTGGTACTGAGCAAc                                <  1:279155/40‑1 (MQ=255)
                                                                      gggACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAAc                                <  1:1054902/40‑1 (MQ=255)
                                                                      gggACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCt                  <  1:1573870/54‑1 (MQ=255)
                                                                      |                                                                     
ACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCA  >  Exported/1439346‑1439485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: