Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,439,429 A→G 17.6% G291G (GGT→GGC ompC ← outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,439,4290AG17.6% 298.5 / 46.9 126G291G (GGT→GGCompCouter membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base A (41/62);  new base G (9/13);  total (51/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

Only 100 of 131 total aligned reads displayed.

TATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTA  >  Exported/1439359‑1439499
                                                                      |                                                                      
tatCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGa                                                                        <  1:2167186/71‑1 (MQ=255)
 atCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGAc                                                                       >  1:4839122/1‑71 (MQ=255)
    ttCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg                                                                     >  1:2203836/1‑70 (MQ=255)
      cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg                                                                     <  1:2602749/68‑1 (MQ=255)
      cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg                                                                     <  1:4553075/68‑1 (MQ=255)
      cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGaa                                                                   <  1:2506492/70‑1 (MQ=255)
      cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGa                                                                    <  1:4591558/69‑1 (MQ=255)
      cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAg                                                                  >  1:1134832/1‑71 (MQ=255)
       gtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGt                                                                 >  1:3647463/1‑71 (MQ=255)
          gtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGa                                                              <  1:1833562/71‑1 (MQ=255)
            cgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAAc                                                            >  1:2020717/1‑71 (MQ=255)
             gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAAc                                                            <  1:1064020/70‑1 (MQ=255)
             gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACt                                                           >  1:4348782/1‑71 (MQ=255)
                gCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTgg                                                         <  1:5136905/70‑1 (MQ=255)
                gCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTgg                                                         <  1:5365246/70‑1 (MQ=255)
                gCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTgg                                                         <  1:4766235/70‑1 (MQ=255)
                   aCGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACt                                                     >  1:4915708/1‑71 (MQ=255)
                          aGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAAc                                              <  1:5095140/71‑1 (MQ=255)
                           ggTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGaa                                                             >  1:5534720/1‑55 (MQ=255)
                           ggTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACa                                             <  1:3138662/71‑1 (MQ=255)
                             tttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGaa                                                                   <  1:2070705/47‑1 (MQ=255)
                              ttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCg                                                               >  1:104976/1‑50 (MQ=255)
                              ttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCg                                                               >  1:152100/1‑50 (MQ=255)
                              ttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCt                                          >  1:5083192/1‑71 (MQ=255)
                              ttttACCTTTAGACTGCAGGTAAGCCAGCGACGGACGCAGACCGAAGTCg                                                               >  1:282336/1‑50 (MQ=255)
                               tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGaa                                                             >  1:5096822/1‑51 (MQ=255)
                               tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGc                                                 <  1:5545305/63‑1 (MQ=255)
                               tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCtt                                         >  1:359277/1‑71 (MQ=255)
                               tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCtt                                         >  1:1710827/1‑71 (MQ=255)
                               tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCtt                                         >  1:3856771/1‑71 (MQ=255)
                               tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCt                                          <  1:2948643/70‑1 (MQ=255)
                               tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCt                                          <  1:1406890/70‑1 (MQ=255)
                               cttACCTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACt                                                           >  1:3008237/2‑53 (MQ=12)
                                ttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGt                                                                 >  1:2741014/1‑46 (MQ=255)
                                 tACCTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACt                                                     >  1:4142245/1‑57 (MQ=12)
                                 tACCTTTAGATTGCAGGTAAGAGATGGACGGGCGCAGGCCAAAGTCGAACTGATACt                                                     >  1:2668514/1‑57 (MQ=255)
                                 tACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCaa                                               >  1:5181823/1‑63 (MQ=255)
                                 tACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTc                                        <  1:4635071/70‑1 (MQ=255)
                                  aCCTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACtt                                         >  1:3224646/1‑68 (MQ=18)
                                  aCCTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTca                                       <  1:1537705/70‑2 (MQ=18)
                                  aCCTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTca                                       <  1:2125179/70‑2 (MQ=18)
                                  aCCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg                                       >  1:3167355/1‑70 (MQ=255)
                                  aCCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg                                       <  1:492745/70‑1 (MQ=255)
                                  aCCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg                                       <  1:2820980/70‑1 (MQ=255)
                                   ccTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTca                                       <  1:2128302/69‑2 (MQ=18)
                                    cTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAg                                    >  1:4586901/1‑71 (MQ=255)
                                    cTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAg                                    >  1:1664479/1‑71 (MQ=255)
                                      ttAGATTGCAGGTAAGCGATGGCCGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGtt                                  >  1:2825957/1‑71 (MQ=16)
                                      ttAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGATGTCGAACTGGTACTGAGCAACAGCTTCGAAGtt                                  >  1:2920638/1‑71 (MQ=255)
                                      ttAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGtt                                  >  1:1420880/1‑71 (MQ=255)
                                      ttAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGt                                   >  1:640514/1‑70 (MQ=255)
                                       tAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGtt                                  <  1:4497202/70‑1 (MQ=16)
                                       tAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGt                                   <  1:1950475/69‑1 (MQ=12)
                                       tAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGtt                                  <  1:4315465/70‑1 (MQ=255)
                                        aGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATa                                                       >  1:2729144/1‑48 (MQ=2)
                                        aGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATa                                                       >  1:2225626/1‑48 (MQ=2)
                                        aGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGttt                                 <  1:1728506/70‑2 (MQ=12)
                                        aGATTGCAGGTAAGCAACGGACGGACGCAGACCGAAATCGAACTGATATTGTGCAACAGCTTCGAAGtttt                                >  1:4744142/1‑69 (MQ=17)
                                        aGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTc                                 <  1:3576373/70‑1 (MQ=255)
                                         gACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCt                                <  1:2782426/70‑1 (MQ=255)
                                           cTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtgtg                             <  1:839806/71‑1 (MQ=255)
                                              cAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAAca                                                           >  1:1757761/1‑37 (MQ=255)
                                              cAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCtt                          <  1:3774929/71‑1 (MQ=255)
                                                 gTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTc                                        <  1:1866340/54‑1 (MQ=9)
                                                 gTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGt                       >  1:5621379/1‑71 (MQ=12)
                                                 gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACt                                                           <  1:305950/35‑1 (MQ=255)
                                                 gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtg                               <  1:4577851/63‑1 (MQ=255)
                                                 gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtg                               <  1:5203468/63‑1 (MQ=255)
                                                  tAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGtt                      <  1:212433/71‑1 (MQ=12)
                                                  tAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGtt                      <  1:4205124/71‑1 (MQ=255)
                                                   aaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACa                                             >  1:741568/1‑47 (MQ=255)
                                                   aaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGc                                           >  1:3181866/1‑49 (MQ=255)
                                                    aGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCg                    >  1:5202142/1‑71 (MQ=255)
                                                       cAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGcc                  >  1:4290362/1‑70 (MQ=255)
                                                       cAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGcc                  >  1:3970357/1‑70 (MQ=255)
                                                        aGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCa                <  1:3738549/71‑1 (MQ=255)
                                                         tggacgggcGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGTTAGc                   <  1:473578/66‑1 (MQ=12)
                                                         gggACGGACGCAGACCGAAGTCTAACTGGTACTGAGCAAc                                              <  1:279155/40‑1 (MQ=255)
                                                         gggACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAAc                                              <  1:5607030/40‑1 (MQ=255)
                                                         gggACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAAc                                              <  1:1054902/40‑1 (MQ=255)
                                                         gggACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAAc                                              <  1:4756293/40‑1 (MQ=255)
                                                         gggACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCt                                <  1:1573870/54‑1 (MQ=255)
                                                          ggacggacGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGtt                                  <  1:525522/51‑1 (MQ=255)
                                                           gacggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAAcc             <  1:888507/71‑1 (MQ=255)
                                                            acggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCa                <  1:5427483/67‑1 (MQ=255)
                                                            acggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCa                <  1:4511539/67‑1 (MQ=255)
                                                            acggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAAccc            <  1:1827755/71‑1 (MQ=255)
                                                             cggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGcc                  <  1:5247982/64‑1 (MQ=255)
                                                              ggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtg                               <  1:5569083/50‑1 (MQ=255)
                                                              ggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAg          <  1:5348626/71‑1 (MQ=255)
                                                                acgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAggg        <  1:4695601/71‑1 (MQ=255)
                                                                acgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAggg        <  1:515938/71‑1 (MQ=255)
                                                                acgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAggg        <  1:4712528/71‑1 (MQ=255)
                                                                acgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAgg         >  1:1440325/1‑70 (MQ=255)
                                                                 cgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtg                               >  1:2599974/1‑47 (MQ=255)
                                                                  gCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGaa      >  1:3235858/1‑71 (MQ=255)
                                                                     gACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCtt                          >  1:3715535/1‑48 (MQ=255)
                                                                      aCCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGcc                  >  1:2363513/1‑55 (MQ=255)
                                                                      aCCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGc                   >  1:3203689/1‑54 (MQ=255)
                                                                      aCCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTa  >  1:2885762/1‑71 (MQ=255)
                                                                      |                                                                      
TATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTA  >  Exported/1439359‑1439499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: