Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,439,432 G→A 17.6% F290F (TTC→TTT ompC ← outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,439,4320GA17.6% 310.0 / 45.9 125F290F (TTC→TTTompCouter membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base G (45/58);  new base A (9/13);  total (54/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

TTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGC  >  Exported/1439363‑1439502
                                                                     |                                                                      
ttCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGCCCg                                                                        >  1:513285/1‑70 (MQ=255)
ttCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg                                                                        >  1:2203836/1‑70 (MQ=255)
  cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg                                                                        <  1:2602749/68‑1 (MQ=255)
  cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg                                                                        <  1:4553075/68‑1 (MQ=255)
  cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg                                                                        <  1:4221174/68‑1 (MQ=255)
  cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg                                                                        <  1:1738365/68‑1 (MQ=255)
  cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGa                                                                       <  1:4591558/69‑1 (MQ=255)
  cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAg                                                                     >  1:1134832/1‑71 (MQ=255)
   gtcgtcgtAGCCACGACCCAGGTTTTTACCTTTGGACTGCAGGTAAGCCAGGGACGGACGCAGACCGaa                                                                      <  1:2625254/69‑1 (MQ=255)
         gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAAc                                                               <  1:1064020/70‑1 (MQ=255)
         gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAAc                                                               <  1:4714787/70‑1 (MQ=255)
         gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACt                                                              >  1:4348782/1‑71 (MQ=255)
         gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGAAGACCGAAGTCGAAc                                                               <  1:3198147/70‑1 (MQ=255)
            gCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTgg                                                            <  1:4766235/70‑1 (MQ=255)
            gCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTgg                                                            <  1:2769725/70‑1 (MQ=255)
            gCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTgg                                                            <  1:5365246/70‑1 (MQ=255)
            gCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTgg                                                            <  1:5136905/70‑1 (MQ=255)
                       ggTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGaa                                                                >  1:5534720/1‑55 (MQ=255)
                       ggTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGaa                                                                >  1:3569540/1‑55 (MQ=255)
                       ggTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACa                                                <  1:3138662/71‑1 (MQ=255)
                         tttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGaa                                                                      <  1:2070705/47‑1 (MQ=255)
                          ttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCg                                                                  >  1:104976/1‑50 (MQ=255)
                          ttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCg                                                                  >  1:152100/1‑50 (MQ=255)
                          ttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCt                                             >  1:5083192/1‑71 (MQ=255)
                           tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGaa                                                                >  1:5096822/1‑51 (MQ=255)
                           tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGc                                                    <  1:5545305/63‑1 (MQ=255)
                           tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCtt                                            >  1:359277/1‑71 (MQ=255)
                           tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCtt                                            >  1:1710827/1‑71 (MQ=255)
                           tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCtt                                            >  1:3856771/1‑71 (MQ=255)
                           tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCt                                             <  1:1406890/70‑1 (MQ=255)
                           tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCt                                             <  1:2948643/70‑1 (MQ=255)
                           cttACCTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACt                                                              >  1:3008237/2‑53 (MQ=12)
                            ttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGt                                                                    >  1:2741014/1‑46 (MQ=255)
                             tACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCaa                                                  >  1:5181823/1‑63 (MQ=255)
                             tACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTc                                           <  1:4635071/70‑1 (MQ=255)
                              aCCTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACtt                                            >  1:3224646/1‑68 (MQ=18)
                              aCCTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTca                                          <  1:1537705/70‑2 (MQ=18)
                              aCCTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTc                                           >  1:207291/1‑69 (MQ=18)
                              aCCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg                                          <  1:2820980/70‑1 (MQ=255)
                              aCCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg                                          <  1:492745/70‑1 (MQ=255)
                              aCCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg                                          >  1:3167355/1‑70 (MQ=255)
                              aCCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg                                          <  1:229860/70‑1 (MQ=255)
                               ccTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTca                                          <  1:2128302/69‑2 (MQ=18)
                                cTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAg                                       >  1:1664479/1‑71 (MQ=255)
                                  ttAGATTGCAGGTAAGCGATGGCCGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGtt                                     >  1:2825957/1‑71 (MQ=16)
                                  ttAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGtt                                     >  1:1420880/1‑71 (MQ=255)
                                  ttAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGt                                      >  1:640514/1‑70 (MQ=255)
                                   tAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGtt                                     <  1:4497202/70‑1 (MQ=16)
                                   tAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGtt                                     <  1:3856330/70‑1 (MQ=16)
                                   tAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGt                                      <  1:1950475/69‑1 (MQ=12)
                                    aGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATa                                                          >  1:2729144/1‑48 (MQ=2)
                                    aGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTc                                    <  1:3576373/70‑1 (MQ=255)
                                       cTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtgtg                                <  1:839806/71‑1 (MQ=255)
                                          cAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAAca                                                              >  1:1757761/1‑37 (MQ=255)
                                            ggTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCt                              <  1:1927208/68‑1 (MQ=255)
                                             gTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTc                                           <  1:1866340/54‑1 (MQ=9)
                                             gTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGt                          >  1:5621379/1‑71 (MQ=12)
                                             gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACt                                                              <  1:1653707/35‑1 (MQ=255)
                                             gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACt                                                              <  1:305950/35‑1 (MQ=255)
                                             gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtg                                  <  1:4577851/63‑1 (MQ=255)
                                             gTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtg                                  <  1:4573596/63‑1 (MQ=255)
                                              tAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGtt                         <  1:5201831/71‑1 (MQ=12)
                                              tAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGtt                         <  1:212433/71‑1 (MQ=12)
                                              tAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGtt                         <  1:1279895/71‑1 (MQ=12)
                                              tAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtg                                  <  1:5024250/62‑1 (MQ=255)
                                              tAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGtt                         <  1:4205124/71‑1 (MQ=255)
                                               aaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACa                                                >  1:741568/1‑47 (MQ=255)
                                               aaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTc                        >  1:2821390/1‑71 (MQ=255)
                                               aaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTc                        >  1:2164181/1‑71 (MQ=255)
                                                aGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCg                       >  1:5202142/1‑71 (MQ=255)
                                                aGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCg                       >  1:3673739/1‑71 (MQ=255)
                                                  ccAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTccca                     <  1:4463286/71‑4 (MQ=255)
                                                   cAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGcc                     >  1:4290362/1‑70 (MQ=255)
                                                   cAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGcc                     >  1:3970357/1‑70 (MQ=255)
                                                    aGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCa                   <  1:3738549/71‑1 (MQ=255)
                                                     tggacgggcGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGTTAGc                      <  1:473578/66‑1 (MQ=12)
                                                     gggACGGACGCAGACCGAAGTCTAACTGGTACTGAGCAAc                                                 <  1:279155/40‑1 (MQ=255)
                                                     gggACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAAc                                                 <  1:5607030/40‑1 (MQ=255)
                                                     gggACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAAc                                                 <  1:1054902/40‑1 (MQ=255)
                                                     gggACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAAc                                                 <  1:4756293/40‑1 (MQ=255)
                                                      ggacggacGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGtt                                     <  1:525522/51‑1 (MQ=255)
                                                       gacggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAAcc                <  1:888507/71‑1 (MQ=255)
                                                        acggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCa                   <  1:5427483/67‑1 (MQ=255)
                                                        acggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAAccc               <  1:477419/71‑1 (MQ=255)
                                                        acggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAAccc               <  1:1827755/71‑1 (MQ=255)
                                                         cggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGcc                     <  1:5247982/64‑1 (MQ=255)
                                                          ggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtg                                  <  1:5569083/50‑1 (MQ=255)
                                                          ggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAg             <  1:5348626/71‑1 (MQ=255)
                                                           gacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAgg            >  1:2896100/1‑71 (MQ=255)
                                                            acgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAggg           <  1:4631618/71‑1 (MQ=255)
                                                            acgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAggg           <  1:4712528/71‑1 (MQ=255)
                                                             cgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtg                                  >  1:2599974/1‑47 (MQ=255)
                                                                 gACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCtt                             >  1:3715535/1‑48 (MQ=255)
                                                                  aCCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGcc                     >  1:2363513/1‑55 (MQ=255)
                                                                  aCCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGc                      >  1:3203689/1‑54 (MQ=255)
                                                                  aCCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCt      >  1:1518147/1‑70 (MQ=255)
                                                                  aCCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTa     >  1:2885762/1‑71 (MQ=255)
                                                                    cGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTAcg   >  1:2961451/1‑71 (MQ=255)
                                                                    cGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTAcg   >  1:242296/1‑71 (MQ=255)
                                                                     gAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTAcgc  <  1:5295041/71‑1 (MQ=255)
                                                                     |                                                                      
TTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGC  >  Exported/1439363‑1439502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: