Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,439,471 T→G 12.7% A277A (GCA→GCC) ‡ ompC ← outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,439,4710TG12.7% 247.7 / 29.2 102A277A (GCA→GCC) ‡ompCouter membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base T (48/41);  new base G (6/7);  total (54/48)
Fisher's exact test for biased strand distribution p-value = 7.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 106 total aligned reads displayed.

CTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAGCAGCCAGGT  >  Exported/1439402‑1439541
                                                                     |                                                                      
cTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtgtg                                                                       <  1:839806/71‑1 (MQ=255)
     ggTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCt                                                                     <  1:1927208/68‑1 (MQ=255)
     ggTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTg                                                                  <  1:5580003/71‑1 (MQ=255)
      gTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGt                                                                 >  1:5621379/1‑71 (MQ=12)
       tAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGtt                                                                <  1:1279895/71‑1 (MQ=12)
       tAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGtt                                                                <  1:5201831/71‑1 (MQ=12)
       tAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGtt                                                                <  1:212433/71‑1 (MQ=12)
       tAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGtt                                                                <  1:4205124/71‑1 (MQ=255)
        aaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTc                                                               >  1:2821390/1‑71 (MQ=255)
        aaGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTc                                                               >  1:2164181/1‑71 (MQ=255)
         aGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCg                                                              >  1:3673739/1‑71 (MQ=255)
         aGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCg                                                              >  1:5202142/1‑71 (MQ=255)
           ccAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTccca                                                            <  1:4463286/71‑4 (MQ=255)
            cAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGcc                                                            >  1:3970357/1‑70 (MQ=255)
            cAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGcc                                                            >  1:4290362/1‑70 (MQ=255)
             aGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCa                                                          <  1:3738549/71‑1 (MQ=255)
              tggacgggcGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGTTAGc                                                             <  1:473578/66‑1 (MQ=12)
                gacggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAAcc                                                       <  1:888507/71‑1 (MQ=255)
                 acggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCa                                                          <  1:5427483/67‑1 (MQ=255)
                 acggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCa                                                          <  1:4511539/67‑1 (MQ=255)
                 acggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAAccc                                                      <  1:1827755/71‑1 (MQ=255)
                 acggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAAccc                                                      <  1:477419/71‑1 (MQ=255)
                  cggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGcc                                                            <  1:5247982/64‑1 (MQ=255)
                   ggacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAg                                                    <  1:5348626/71‑1 (MQ=255)
                    gacgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAgg                                                   >  1:2896100/1‑71 (MQ=255)
                     acgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAggg                                                  <  1:4695601/71‑1 (MQ=255)
                     acgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAggg                                                  <  1:4712528/71‑1 (MQ=255)
                     acgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAggg                                                  <  1:515938/71‑1 (MQ=255)
                     acgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAggg                                                  <  1:4631618/71‑1 (MQ=255)
                     acgCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAgg                                                   >  1:1440325/1‑70 (MQ=255)
                       gCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGaa                                                >  1:3235858/1‑71 (MQ=255)
                          gACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCtt                                                                    >  1:3715535/1‑48 (MQ=255)
                           aCCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGcc                                                            >  1:2363513/1‑55 (MQ=255)
                           aCCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGc                                                             >  1:3203689/1‑54 (MQ=255)
                           aCCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCt                                             >  1:1518147/1‑70 (MQ=255)
                             cGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTAcg                                          >  1:242296/1‑71 (MQ=255)
                             cGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTAcg                                          >  1:2961451/1‑71 (MQ=255)
                              gAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTAcgc                                         <  1:1203482/71‑1 (MQ=255)
                              gAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTAcgc                                         <  1:5295041/71‑1 (MQ=255)
                               aaGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTAcgcg                                        <  1:2877821/71‑1 (MQ=255)
                               aaGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTAcgcg                                        <  1:2871320/71‑1 (MQ=255)
                               aaGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTAcgc                                         >  1:309436/1‑70 (MQ=255)
                               aaGTCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGtt                                                                >  1:1954970/1‑47 (MQ=19)
                                 gTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAg                                      <  1:548413/71‑1 (MQ=255)
                                  tCGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGt                                                                 <  1:4932016/43‑1 (MQ=16)
                                   cGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGtt                                    >  1:4982332/1‑71 (MQ=255)
                                   cGAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGTTAGcga                                                           >  1:5212954/1‑46 (MQ=16)
                                    gAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGtt                                    >  1:5099683/1‑70 (MQ=255)
                                    gAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGttag                                                              >  1:263830/1‑42 (MQ=12)
                                    gAACTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGttag                                                              >  1:4403302/1‑42 (MQ=12)
                                     aaCTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGc                                  >  1:3425462/1‑71 (MQ=255)
                                      aCTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCg                                 >  1:964801/1‑71 (MQ=255)
                                      aCTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGTTAGc                                                             <  1:5258728/43‑1 (MQ=12)
                                      aCTGATACTGAGCAACAACTTCAAAGTTTTGGGTTTTGTTAGc                                                             <  1:2531925/43‑1 (MQ=12)
                                       cTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAg                                                    >  1:799047/1‑51 (MQ=255)
                                       cTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGa                                       >  1:667362/1‑64 (MQ=255)
                                         ggTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGt                                                                 >  1:260644/1‑36 (MQ=255)
                                         ggTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTg                              >  1:5012699/1‑71 (MQ=255)
                                             cTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAgg                          >  1:5360290/1‑71 (MQ=255)
                                             cTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAg                           <  1:2328281/70‑1 (MQ=255)
                                               gAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGa                                       >  1:2398863/1‑56 (MQ=255)
                                               gAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTc                        >  1:5426898/1‑71 (MQ=255)
                                               gAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGACTTGCGTTGTAGGTc                        >  1:240772/1‑71 (MQ=255)
                                                aGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGt                         <  1:4733332/69‑1 (MQ=255)
                                                aGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGt                         <  1:606913/69‑1 (MQ=255)
                                                    aCAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTAcac                                         <  1:2218617/49‑3 (MQ=255)
                                                    aCAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTAcac                                         <  1:669145/49‑3 (MQ=255)
                                                    aCAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAg                                      <  1:5049327/52‑1 (MQ=255)
                                                    aCAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAg                                      <  1:3520154/52‑1 (MQ=255)
                                                     cAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTa                                            <  1:2003798/45‑1 (MQ=255)
                                                     cAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGgtg                  <  1:3756049/71‑1 (MQ=255)
                                                     cAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGgtg                  >  1:4918357/1‑71 (MQ=255)
                                                     cAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGgtg                  >  1:2475661/1‑71 (MQ=255)
                                                      tgCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAg                                                    >  1:5155091/2‑36 (MQ=255)
                                                      aGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCa                                                     >  1:3086118/1‑35 (MQ=255)
                                                      aGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCa                                                     >  1:340815/1‑35 (MQ=255)
                                                      aGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGgtgt                 <  1:402799/71‑1 (MQ=255)
                                                      aGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGgtg                  <  1:4885115/70‑1 (MQ=255)
                                                       gCTTCGAATTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTa                >  1:1782947/1‑71 (MQ=255)
                                                       gCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTa                >  1:2002851/1‑71 (MQ=255)
                                                        cTTCGAAGTTCTGTGCTTTGTTCTCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTAc               <  1:1516407/71‑1 (MQ=255)
                                                        cTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCg                                 <  1:407557/53‑1 (MQ=255)
                                                          tCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACt              >  1:3710470/1‑70 (MQ=255)
                                                           cGAAGTTCTGTGCTTTGTTCGCCCAACCCATGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTAc               >  1:3775049/1‑68 (MQ=255)
                                                           cGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACt              <  1:2499977/69‑1 (MQ=255)
                                                            gAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGgt                   >  1:5518644/1‑63 (MQ=255)
                                                            gAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGag           >  1:3892365/1‑71 (MQ=255)
                                                            gAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGag           >  1:4792552/1‑71 (MQ=255)
                                                              aGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACt              >  1:907154/1‑66 (MQ=255)
                                                                ttCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTg                              <  1:3555233/48‑1 (MQ=255)
                                                                ttCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGt                             <  1:2082820/49‑1 (MQ=255)
                                                                  cTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTa                            >  1:611593/1‑48 (MQ=255)
                                                                  cTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGagcagc       >  1:3553910/1‑69 (MQ=255)
                                                                  cTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGagcagc       >  1:2253485/1‑69 (MQ=255)
                                                                  cTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGagcagc       >  1:717388/1‑69 (MQ=255)
                                                                  cTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGagcagc       >  1:1679602/1‑69 (MQ=255)
                                                                  cTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAGCAGcc      <  1:2287588/70‑1 (MQ=255)
                                                                     tgCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGagcag        <  1:2149975/65‑1 (MQ=255)
                                                                     tgCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAGCAGCCa     <  1:1332732/68‑1 (MQ=255)
                                                                     tgCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAGCAGCCAGGt  <  1:2473749/71‑1 (MQ=255)
                                                                     |                                                                      
CTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTGCTTTGTTCGCCCAACCCAGGGAACCTACGCGAGTTGCGTTGTAGGTCTGGGTGTACTGAGCAGCCAGGT  >  Exported/1439402‑1439541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: