Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | Exported | 1,439,935 | A→C | 8.3% | S123A (TCC→GCC) ‡ | ompC ← | outer membrane porin protein C |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | Exported | 1,439,935 | 0 | A | C | 8.3% | 402.0 / 25.4 | 145 | S123A (TCC→GCC) ‡ | ompC | outer membrane porin protein C |
Reads supporting (aligned to +/- strand): ref base A (60/73); new base C (6/6); total (66/79) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.71e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.98e-01 |
Only 100 of 151 total aligned reads displayed.
CCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACC > Exported/1439865‑1440004 | ccACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGa < 1:2856428/71‑1 (MQ=255) aCGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAg > 1:4921759/1‑71 (MQ=255) cGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt < 1:2699539/71‑1 (MQ=255) cGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt > 1:568697/1‑71 (MQ=255) cGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt > 1:1523978/1‑71 (MQ=255) gctgctGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt > 1:5368873/1‑70 (MQ=255) gctgcATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACg > 1:802831/1‑71 (MQ=255) ctgcATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTAc < 1:2587952/69‑1 (MQ=255) gcATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGt > 1:2224268/1‑69 (MQ=255) cATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGa < 1:5079025/60‑1 (MQ=255) cATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCAt < 1:1193730/71‑1 (MQ=255) aTGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATa < 1:72602/71‑1 (MQ=255) aTGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATa < 1:3702901/71‑1 (MQ=255) aaGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATaaa > 1:2516699/1‑70 (MQ=255) aGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGa < 1:3291524/55‑1 (MQ=255) gTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt < 1:5087546/57‑1 (MQ=255) gTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt < 1:5234026/57‑1 (MQ=255) gTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaaca > 1:4046471/1‑70 (MQ=255) gTTGTCAGAACCGTAGGTGTAACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt < 1:1776519/57‑1 (MQ=255) tGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATa > 1:1679774/1‑64 (MQ=255) tGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATa > 1:4225953/1‑64 (MQ=255) gTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACg > 1:534128/1‑70 (MQ=255) gTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGc > 1:5161832/1‑71 (MQ=255) gTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGc > 1:5586634/1‑71 (MQ=255) gTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGc > 1:77581/1‑71 (MQ=255) gTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGc > 1:5414727/1‑71 (MQ=255) cAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGaa > 1:2320880/1‑50 (MQ=255) aGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGt < 1:3815406/71‑1 (MQ=255) aGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGt < 1:4179201/71‑1 (MQ=255) gAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGt < 1:4013174/55‑1 (MQ=255) aCCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt < 1:5497187/48‑1 (MQ=255) ccGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGt > 1:3274189/1‑70 (MQ=255) tAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGt < 1:2183552/64‑1 (MQ=255) tAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTAc < 1:5600500/70‑1 (MQ=255) tAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACg > 1:485216/1‑71 (MQ=255) tAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACg < 1:1385996/71‑1 (MQ=255) ggTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATa > 1:2282188/1‑51 (MQ=255) ggTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATa > 1:3964201/1‑51 (MQ=255) gtCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCg > 1:1314500/1‑70 (MQ=255) gccaccaAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGt > 1:1029332/2‑71 (MQ=2) accaccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTAc < 1:4869008/39‑1 (MQ=255) accaccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAg < 1:234209/70‑1 (MQ=255) ccaccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTAc < 1:2815381/38‑1 (MQ=255) ccaccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCAt < 1:2677133/43‑1 (MQ=255) ccaccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCAt < 1:1387875/43‑1 (MQ=255) ccaccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac < 1:4796874/47‑1 (MQ=255) caccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTc > 1:3122079/1‑70 (MQ=255) caccAAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTc > 1:3933879/1‑70 (MQ=2) accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATaa > 1:594004/1‑43 (MQ=255) accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATaa > 1:1125920/1‑43 (MQ=255) accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATaa < 1:541706/43‑1 (MQ=255) accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATaa > 1:2982612/1‑43 (MQ=255) accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATaa < 1:2054869/43‑1 (MQ=255) accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGAcc > 1:5221540/1‑63 (MQ=255) accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGa < 1:3548694/71‑1 (MQ=255) accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGa < 1:1899483/71‑1 (MQ=255) accGAATTATGGCAGTACGTCGGTACAGGAAGTTACGTCATAaacaa > 1:3301902/1‑47 (MQ=255) accAAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCg > 1:2077768/1‑70 (MQ=2) accAAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGa < 1:3858876/71‑1 (MQ=2) accAAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGa < 1:2183233/71‑1 (MQ=2) ccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac < 1:1343961/44‑1 (MQ=255) cGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac < 1:1031628/43‑1 (MQ=255) cGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac < 1:2006932/43‑1 (MQ=255) cGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac < 1:231051/43‑1 (MQ=255) cGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac < 1:2420399/43‑1 (MQ=255) cGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac < 1:3477313/43‑1 (MQ=255) gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATaaa > 1:4436782/1‑41 (MQ=255) gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTa > 1:1664598/1‑55 (MQ=255) gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTa > 1:4681827/1‑55 (MQ=255) gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTa > 1:2565344/1‑55 (MQ=255) gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTa > 1:3116911/1‑55 (MQ=255) gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGa > 1:1224023/1‑68 (MQ=255) gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAg < 1:4759651/71‑1 (MQ=255) aaaTTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGa < 1:1639409/67‑1 (MQ=9) aaTTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaaca > 1:5266472/1‑42 (MQ=255) ttCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac < 1:5393229/39‑1 (MQ=255) ttCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATa < 1:2916126/60‑1 (MQ=2) ttCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATa < 1:1568840/60‑1 (MQ=2) cTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTa > 1:357278/1‑46 (MQ=255) cTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTa < 1:1653329/58‑1 (MQ=255) cTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTa < 1:2263981/58‑1 (MQ=255) cTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCCTa < 1:4663147/58‑1 (MQ=255) gTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGt < 1:5207718/42‑1 (MQ=255) gTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTAc < 1:3922148/45‑1 (MQ=255) gTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGa < 1:4038902/61‑1 (MQ=255) tACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTATTTACGACCGTAGTCGAAAGAACCCACATcc < 1:5483669/70‑1 (MQ=255) tACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCg < 1:379425/55‑1 (MQ=255) tACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCg < 1:5182644/55‑1 (MQ=255) tACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGaa < 1:4545436/57‑1 (MQ=255) tACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGaa < 1:748351/57‑1 (MQ=255) aCGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGt < 1:3183425/40‑1 (MQ=255) aCGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAAc > 1:1853459/1‑61 (MQ=255) aCGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTg > 1:855759/1‑71 (MQ=255) cGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTgg < 1:4442729/71‑1 (MQ=255) tCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAg < 1:1064637/55‑1 (MQ=255) ggTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAAtt < 1:4948393/71‑1 (MQ=255) ggTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAAt < 1:3756184/70‑1 (MQ=255) ggTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAAt < 1:3516121/70‑1 (MQ=255) cAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAg > 1:4526654/1‑49 (MQ=255) gAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGAcc > 1:5313212/1‑71 (MQ=255) | CCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACC > Exported/1439865‑1440004 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |