Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,439,935 A→C 8.3% S123A (TCC→GCC) ‡ ompC ← outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,439,9350AC 8.3% 402.0 / 25.4 145S123A (TCC→GCC) ‡ompCouter membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base A (60/73);  new base C (6/6);  total (66/79)
Fisher's exact test for biased strand distribution p-value = 7.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.98e-01

Only 100 of 151 total aligned reads displayed.

CCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACC  >  Exported/1439865‑1440004
                                                                      |                                                                     
ccACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGa                                                                       <  1:2856428/71‑1 (MQ=255)
  aCGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAg                                                                     >  1:4921759/1‑71 (MQ=255)
   cGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt                                                                    <  1:2699539/71‑1 (MQ=255)
   cGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt                                                                    >  1:568697/1‑71 (MQ=255)
   cGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt                                                                    >  1:1523978/1‑71 (MQ=255)
    gctgctGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt                                                                    >  1:5368873/1‑70 (MQ=255)
       gctgcATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACg                                                                >  1:802831/1‑71 (MQ=255)
        ctgcATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTAc                                                                 <  1:2587952/69‑1 (MQ=255)
          gcATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGt                                                               >  1:2224268/1‑69 (MQ=255)
           cATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGa                                                                       <  1:5079025/60‑1 (MQ=255)
           cATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCAt                                                            <  1:1193730/71‑1 (MQ=255)
            aTGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATa                                                           <  1:72602/71‑1 (MQ=255)
            aTGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATa                                                           <  1:3702901/71‑1 (MQ=255)
               aaGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATaaa                                                         >  1:2516699/1‑70 (MQ=255)
                aGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGa                                                                       <  1:3291524/55‑1 (MQ=255)
                 gTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt                                                                    <  1:5087546/57‑1 (MQ=255)
                 gTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt                                                                    <  1:5234026/57‑1 (MQ=255)
                 gTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaaca                                                       >  1:4046471/1‑70 (MQ=255)
                 gTTGTCAGAACCGTAGGTGTAACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt                                                                    <  1:1776519/57‑1 (MQ=255)
                   tGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATa                                                           >  1:1679774/1‑64 (MQ=255)
                   tGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATa                                                           >  1:4225953/1‑64 (MQ=255)
                    gTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACg                                                    >  1:534128/1‑70 (MQ=255)
                    gTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGc                                                   >  1:5161832/1‑71 (MQ=255)
                    gTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGc                                                   >  1:5586634/1‑71 (MQ=255)
                    gTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGc                                                   >  1:77581/1‑71 (MQ=255)
                    gTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGc                                                   >  1:5414727/1‑71 (MQ=255)
                      cAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGaa                                                                      >  1:2320880/1‑50 (MQ=255)
                       aGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGt                                                <  1:3815406/71‑1 (MQ=255)
                       aGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGt                                                <  1:4179201/71‑1 (MQ=255)
                        gAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGt                                                               <  1:4013174/55‑1 (MQ=255)
                          aCCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt                                                                    <  1:5497187/48‑1 (MQ=255)
                           ccGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGt                                             >  1:3274189/1‑70 (MQ=255)
                              tAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGt                                                <  1:2183552/64‑1 (MQ=255)
                              tAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTAc                                          <  1:5600500/70‑1 (MQ=255)
                              tAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACg                                         >  1:485216/1‑71 (MQ=255)
                              tAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACg                                         <  1:1385996/71‑1 (MQ=255)
                                ggTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATa                                                           >  1:2282188/1‑51 (MQ=255)
                                ggTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATa                                                           >  1:3964201/1‑51 (MQ=255)
                                   gtCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCg                                     >  1:1314500/1‑70 (MQ=255)
                                      gccaccaAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGt                                 >  1:1029332/2‑71 (MQ=2)
                                      accaccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTAc                                                                 <  1:4869008/39‑1 (MQ=255)
                                      accaccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAg                                  <  1:234209/70‑1 (MQ=255)
                                       ccaccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTAc                                                                 <  1:2815381/38‑1 (MQ=255)
                                       ccaccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCAt                                                            <  1:2677133/43‑1 (MQ=255)
                                       ccaccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCAt                                                            <  1:1387875/43‑1 (MQ=255)
                                       ccaccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac                                                        <  1:4796874/47‑1 (MQ=255)
                                        caccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTc                                >  1:3122079/1‑70 (MQ=255)
                                        caccAAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTc                                >  1:3933879/1‑70 (MQ=2)
                                         accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATaa                                                          >  1:594004/1‑43 (MQ=255)
                                         accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATaa                                                          >  1:1125920/1‑43 (MQ=255)
                                         accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATaa                                                          <  1:541706/43‑1 (MQ=255)
                                         accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATaa                                                          >  1:2982612/1‑43 (MQ=255)
                                         accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATaa                                                          <  1:2054869/43‑1 (MQ=255)
                                         accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGAcc                                      >  1:5221540/1‑63 (MQ=255)
                                         accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGa                              <  1:3548694/71‑1 (MQ=255)
                                         accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGa                              <  1:1899483/71‑1 (MQ=255)
                                         accGAATTATGGCAGTACGTCGGTACAGGAAGTTACGTCATAaacaa                                                      >  1:3301902/1‑47 (MQ=255)
                                         accAAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCg                               >  1:2077768/1‑70 (MQ=2)
                                         accAAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGa                              <  1:3858876/71‑1 (MQ=2)
                                         accAAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGa                              <  1:2183233/71‑1 (MQ=2)
                                          ccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac                                                        <  1:1343961/44‑1 (MQ=255)
                                           cGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac                                                        <  1:1031628/43‑1 (MQ=255)
                                           cGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac                                                        <  1:2006932/43‑1 (MQ=255)
                                           cGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac                                                        <  1:231051/43‑1 (MQ=255)
                                           cGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac                                                        <  1:2420399/43‑1 (MQ=255)
                                           cGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac                                                        <  1:3477313/43‑1 (MQ=255)
                                            gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATaaa                                                         >  1:4436782/1‑41 (MQ=255)
                                            gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTa                                           >  1:1664598/1‑55 (MQ=255)
                                            gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTa                                           >  1:4681827/1‑55 (MQ=255)
                                            gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTa                                           >  1:2565344/1‑55 (MQ=255)
                                            gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTa                                           >  1:3116911/1‑55 (MQ=255)
                                            gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGa                              >  1:1224023/1‑68 (MQ=255)
                                            gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAg                           <  1:4759651/71‑1 (MQ=255)
                                            aaaTTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGa                              <  1:1639409/67‑1 (MQ=9)
                                             aaTTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaaca                                                       >  1:5266472/1‑42 (MQ=255)
                                               ttCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac                                                        <  1:5393229/39‑1 (MQ=255)
                                               ttCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATa                                   <  1:2916126/60‑1 (MQ=2)
                                               ttCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATa                                   <  1:1568840/60‑1 (MQ=2)
                                                 cTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTa                                               >  1:357278/1‑46 (MQ=255)
                                                 cTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTa                                   <  1:1653329/58‑1 (MQ=255)
                                                 cTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTa                                   <  1:2263981/58‑1 (MQ=255)
                                                 cTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCCTa                                   <  1:4663147/58‑1 (MQ=255)
                                                       gTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGt                                             <  1:5207718/42‑1 (MQ=255)
                                                       gTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTAc                                          <  1:3922148/45‑1 (MQ=255)
                                                       gTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGa                          <  1:4038902/61‑1 (MQ=255)
                                                        tACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTATTTACGACCGTAGTCGAAAGAACCCACATcc                <  1:5483669/70‑1 (MQ=255)
                                                        tACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCg                               <  1:379425/55‑1 (MQ=255)
                                                        tACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCg                               <  1:5182644/55‑1 (MQ=255)
                                                        tACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGaa                             <  1:4545436/57‑1 (MQ=255)
                                                        tACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGaa                             <  1:748351/57‑1 (MQ=255)
                                                         aCGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGt                                             <  1:3183425/40‑1 (MQ=255)
                                                         aCGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAAc                        >  1:1853459/1‑61 (MQ=255)
                                                         aCGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTg              >  1:855759/1‑71 (MQ=255)
                                                          cGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTgg             <  1:4442729/71‑1 (MQ=255)
                                                            tCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAg                           <  1:1064637/55‑1 (MQ=255)
                                                              ggTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAAtt         <  1:4948393/71‑1 (MQ=255)
                                                              ggTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAAt          <  1:3756184/70‑1 (MQ=255)
                                                              ggTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAAt          <  1:3516121/70‑1 (MQ=255)
                                                                  cAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAg                           >  1:4526654/1‑49 (MQ=255)
                                                                     gAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGAcc  >  1:5313212/1‑71 (MQ=255)
                                                                      |                                                                     
CCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACC  >  Exported/1439865‑1440004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: