| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,439,936 | A→T | 8.2% | T122T (ACT→ACA) ‡ | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,439,936 | 0 | A | T | 8.2% | 412.2 / 21.0 | 145 | T122T (ACT→ACA) ‡ | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base A (60/73); new base T (6/6); total (66/79) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01 | |||||||||||
Only 100 of 149 total aligned reads displayed.
ACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACC > Exported/1439867‑1440004 | cGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt > 1:568697/1‑71 (MQ=255) cGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt > 1:169238/1‑71 (MQ=255) cGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt > 1:4980864/1‑71 (MQ=255) gctgctGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt > 1:5368873/1‑70 (MQ=255) gctgcATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACg > 1:455742/1‑71 (MQ=255) ctgcATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACg < 1:644195/70‑1 (MQ=255) gcATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGt > 1:5229034/1‑69 (MQ=255) gcATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGt > 1:2224268/1‑69 (MQ=255) cATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCAt < 1:1193730/71‑1 (MQ=255) cATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCAt < 1:5096470/71‑1 (MQ=255) aTGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATa < 1:3702901/71‑1 (MQ=255) aTGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATa < 1:72602/71‑1 (MQ=255) aaGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt > 1:4028494/1‑59 (MQ=255) aaGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATaaa > 1:1659238/1‑70 (MQ=255) gTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt < 1:5234026/57‑1 (MQ=255) gTTGTCAGAACCGTAGGTGTAACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt < 1:1776519/57‑1 (MQ=255) gTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGc > 1:5414727/1‑71 (MQ=255) gTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGc > 1:5161832/1‑71 (MQ=255) gTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGc > 1:77581/1‑71 (MQ=255) gTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGc > 1:5192443/1‑71 (MQ=255) gTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGc > 1:5586634/1‑71 (MQ=255) cAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGaa > 1:2320880/1‑50 (MQ=255) aGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGt < 1:3815406/71‑1 (MQ=255) aGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGt < 1:4179201/71‑1 (MQ=255) aCCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt < 1:2129100/48‑1 (MQ=255) ccGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGt > 1:3274189/1‑70 (MQ=255) cGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTa > 1:1367890/1‑48 (MQ=255) gTAGGTGTCGCCACCGAATTCTGGCAGAACGTCGGTCCAGGAAGTTACGTCGTAAACAACGCCGTAGTTAc > 1:5286585/1‑71 (MQ=255) ggTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATa > 1:2282188/1‑51 (MQ=255) ggTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATa > 1:3964201/1‑51 (MQ=255) gtCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGtt > 1:2382272/1‑63 (MQ=255) gccaccaAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGt > 1:1029332/2‑71 (MQ=2) accaccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTAc < 1:4869008/39‑1 (MQ=255) accaccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTAc < 1:755587/39‑1 (MQ=255) ccaccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTAc < 1:2815381/38‑1 (MQ=255) ccaccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCAt < 1:2677133/43‑1 (MQ=255) ccaccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCAt < 1:1387875/43‑1 (MQ=255) ccaccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac < 1:4796874/47‑1 (MQ=255) caccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTAc > 1:2317450/1‑60 (MQ=255) caccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTc > 1:3122079/1‑70 (MQ=255) accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATaa < 1:4747159/43‑1 (MQ=255) accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATaa > 1:2982612/1‑43 (MQ=255) accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATaa < 1:2054869/43‑1 (MQ=255) accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATaa > 1:1125920/1‑43 (MQ=255) accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGa > 1:2560936/1‑61 (MQ=255) accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGAcc > 1:5682205/1‑63 (MQ=255) accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGa < 1:3548694/71‑1 (MQ=255) accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGa < 1:1899483/71‑1 (MQ=255) accGAATTATGGCAGTACGTCGGTACAGGAAGTTACGTCATAaacaa > 1:3301902/1‑47 (MQ=255) accAAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGa < 1:2183233/71‑1 (MQ=2) accAAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGa < 1:3865498/71‑1 (MQ=2) ccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac < 1:5397797/44‑1 (MQ=255) ccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac < 1:4828836/44‑1 (MQ=255) ccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac < 1:1343961/44‑1 (MQ=255) caaaTTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGa > 1:2805084/3‑69 (MQ=2) cGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac < 1:3477313/43‑1 (MQ=255) cGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac < 1:2420399/43‑1 (MQ=255) cGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac < 1:1031628/43‑1 (MQ=255) gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTa > 1:3116911/1‑55 (MQ=255) gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTa > 1:2565344/1‑55 (MQ=255) gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTa > 1:4875436/1‑55 (MQ=255) gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTa > 1:1664598/1‑55 (MQ=255) gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTa > 1:4681827/1‑55 (MQ=255) gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGa > 1:1224023/1‑68 (MQ=255) aaaTTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGa < 1:1639409/67‑1 (MQ=9) aaaTTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGAAAg > 1:4231383/2‑71 (MQ=2) ttCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTAc < 1:259777/53‑1 (MQ=255) ttCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATa < 1:2916126/60‑1 (MQ=2) ttCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATa < 1:1568840/60‑1 (MQ=2) cTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac < 1:3709835/37‑1 (MQ=255) cTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac < 1:4153494/37‑1 (MQ=255) cTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTa > 1:357278/1‑46 (MQ=255) cTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGaaa > 1:3960016/1‑65 (MQ=2) gTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGt < 1:5207718/42‑1 (MQ=255) gTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTAc < 1:3922148/45‑1 (MQ=255) gTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGa < 1:4038902/61‑1 (MQ=255) gTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATcc < 1:556987/71‑1 (MQ=255) tACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTATTTACGACCGTAGTCGAAAGAACCCACATcc < 1:5483669/70‑1 (MQ=255) tACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGaa < 1:4545436/57‑1 (MQ=255) tACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGaa < 1:379437/57‑1 (MQ=255) aCGTCGGTCCAGGAAGTTACGTCATAACCAACGCCGTAGTTa > 1:2268116/1‑42 (MQ=255) aCGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGt < 1:3183425/40‑1 (MQ=255) aCGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTa > 1:5438572/1‑42 (MQ=255) aCGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTg > 1:855759/1‑71 (MQ=255) cGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTgg < 1:4442729/71‑1 (MQ=255) cGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTg < 1:1887784/70‑1 (MQ=255) cGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTg < 1:4490468/70‑1 (MQ=255) tCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAg < 1:1407141/55‑1 (MQ=255) tCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTg < 1:5367911/68‑1 (MQ=255) ggTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAAtt < 1:4948393/71‑1 (MQ=255) ggTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAAt < 1:3516121/70‑1 (MQ=255) ggTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAAt < 1:3756184/70‑1 (MQ=255) gTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAAttt > 1:803002/1‑71 (MQ=255) cAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAAttt > 1:2205639/1‑68 (MQ=255) cAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAAttt > 1:1061861/1‑68 (MQ=255) gAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGAcc > 1:5313212/1‑71 (MQ=255) gAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGAcc > 1:2011459/1‑71 (MQ=255) aGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGt > 1:3406862/1‑38 (MQ=255) aGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGt > 1:1089521/1‑38 (MQ=255) aGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGaa > 1:4401152/1‑60 (MQ=255) | ACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACC > Exported/1439867‑1440004 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |