Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,439,938 T→C 8.9% T122A (ACT→GCT) ‡ ompC ← outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,439,9380TC 8.9% 376.7 / 18.9 135T122A (ACT→GCT) ‡ompCouter membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base T (56/67);  new base C (6/6);  total (62/73)
Fisher's exact test for biased strand distribution p-value = 7.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 151 total aligned reads displayed.

CGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCG  >  Exported/1439868‑1440008
                                                                      |                                                                      
cGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt                                                                        >  1:4980864/1‑71 (MQ=255)
cGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt                                                                        <  1:2699539/71‑1 (MQ=255)
cGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt                                                                        >  1:568697/1‑71 (MQ=255)
cGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt                                                                        >  1:1523978/1‑71 (MQ=255)
 gctgctGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt                                                                        >  1:5368873/1‑70 (MQ=255)
    gctgcATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACg                                                                    >  1:802831/1‑71 (MQ=255)
     ctgcATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTAc                                                                     <  1:2587952/69‑1 (MQ=255)
     ctgcATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACg                                                                    <  1:644195/70‑1 (MQ=255)
       gcATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGt                                                                   >  1:2224268/1‑69 (MQ=255)
       gcATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGt                                                                   >  1:5229034/1‑69 (MQ=255)
        cATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCAt                                                                <  1:5096470/71‑1 (MQ=255)
            aaGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt                                                                        >  1:4028494/1‑59 (MQ=255)
            aaGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATaaa                                                             >  1:2516699/1‑70 (MQ=255)
            aaGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac                                                            <  1:3136282/71‑1 (MQ=255)
              gTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt                                                                        <  1:5234026/57‑1 (MQ=255)
              gTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaaca                                                           >  1:4046471/1‑70 (MQ=255)
              gTTGTCAGAACCGTAGGTGTAACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt                                                                        <  1:1776519/57‑1 (MQ=255)
                tGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATa                                                               >  1:1679774/1‑64 (MQ=255)
                tGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATa                                                               >  1:4225953/1‑64 (MQ=255)
                 gTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACg                                                        <  1:2857174/70‑1 (MQ=255)
                 gTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGc                                                       >  1:5586634/1‑71 (MQ=255)
                 gTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGc                                                       >  1:5414727/1‑71 (MQ=255)
                 gTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGc                                                       >  1:5192443/1‑71 (MQ=255)
                 gTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGc                                                       >  1:5161832/1‑71 (MQ=255)
                     gAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGt                                                                   <  1:4013174/55‑1 (MQ=255)
                       aCCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGt                                                                        <  1:2129100/48‑1 (MQ=255)
                        ccGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGt                                                 >  1:3274189/1‑70 (MQ=255)
                         cGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTa                                                                      >  1:1367890/1‑48 (MQ=255)
                          gTAGGTGTCGCCACCGAATTCTGGCAGAACGTCGGTCCAGGAAGTTACGTCGTAAACAACGCCGTAGTTAc                                              >  1:5286585/1‑71 (MQ=255)
                           tAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACg                                             <  1:1385996/71‑1 (MQ=255)
                           tAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACg                                             >  1:485216/1‑71 (MQ=255)
                             ggTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATa                                                               >  1:2282188/1‑51 (MQ=255)
                                gtCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCg                                         >  1:1314500/1‑70 (MQ=255)
                                   gccaccaAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGt                                     >  1:1029332/2‑71 (MQ=2)
                                   accaccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTAc                                                                     <  1:4869008/39‑1 (MQ=255)
                                   accaccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTAc                                                                     <  1:755587/39‑1 (MQ=255)
                                   accaccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAg                                      <  1:234209/70‑1 (MQ=255)
                                    ccaccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTAc                                                                     <  1:2815381/38‑1 (MQ=255)
                                    ccaccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCAt                                                                <  1:2677133/43‑1 (MQ=255)
                                    ccaccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac                                                            <  1:4796874/47‑1 (MQ=255)
                                     caccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTAc                                              >  1:2317450/1‑60 (MQ=255)
                                     caccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTc                                    >  1:3122079/1‑70 (MQ=255)
                                     caccAAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTc                                    >  1:3933879/1‑70 (MQ=2)
                                      accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATaa                                                              <  1:2054869/43‑1 (MQ=255)
                                      accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATaa                                                              >  1:594004/1‑43 (MQ=255)
                                      accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATaa                                                              >  1:2982612/1‑43 (MQ=255)
                                      accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGAcc                                          >  1:5682205/1‑63 (MQ=255)
                                      accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGa                                  <  1:1899483/71‑1 (MQ=255)
                                      accAAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGa                                  <  1:3865498/71‑1 (MQ=2)
                                      accAAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGa                                  <  1:2183233/71‑1 (MQ=2)
                                      accAAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGa                                  <  1:3858876/71‑1 (MQ=2)
                                       ccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATa                                                               <  1:3431447/41‑1 (MQ=255)
                                       ccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac                                                            <  1:5397797/44‑1 (MQ=255)
                                        cGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac                                                            <  1:1031628/43‑1 (MQ=255)
                                        cGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac                                                            <  1:3275574/43‑1 (MQ=255)
                                        cGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac                                                            <  1:2420399/43‑1 (MQ=255)
                                         gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATaaa                                                             >  1:4436782/1‑41 (MQ=255)
                                         gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTa                                               >  1:2565344/1‑55 (MQ=255)
                                         gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTa                                               >  1:1664598/1‑55 (MQ=255)
                                         gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTa                                               >  1:4681827/1‑55 (MQ=255)
                                         gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGa                                  >  1:1224023/1‑68 (MQ=255)
                                         gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAg                               <  1:4759651/71‑1 (MQ=255)
                                         aaaTTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGa                                  <  1:1639409/67‑1 (MQ=9)
                                         aaaTTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGAAAg                               >  1:4231383/2‑71 (MQ=2)
                                            ttCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac                                                            <  1:3525080/39‑1 (MQ=255)
                                              cTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac                                                            <  1:3709835/37‑1 (MQ=255)
                                              cTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaac                                                            <  1:4153494/37‑1 (MQ=255)
                                              cTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTa                                                   >  1:357278/1‑46 (MQ=255)
                                              cTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTa                                       <  1:1653329/58‑1 (MQ=255)
                                              cTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCCTa                                       <  1:4663147/58‑1 (MQ=255)
                                              cTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGaaa                                >  1:3960016/1‑65 (MQ=2)
                                                    gTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGt                                                 <  1:5207718/42‑1 (MQ=255)
                                                    gTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATcc                    <  1:556987/71‑1 (MQ=255)
                                                     tACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTATTTACGACCGTAGTCGAAAGAACCCACATcc                    <  1:5483669/70‑1 (MQ=255)
                                                     tACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCg                                   <  1:379425/55‑1 (MQ=255)
                                                     tACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGaa                                 <  1:379437/57‑1 (MQ=255)
                                                     tACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGaa                                 <  1:748351/57‑1 (MQ=255)
                                                     tACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGaa                                 <  1:4545436/57‑1 (MQ=255)
                                                      aCGTCGGTCCAGGAAGTTACGTCATAACCAACGCCGTAGTTa                                               >  1:2268116/1‑42 (MQ=255)
                                                      aCGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTa                                               >  1:5438572/1‑42 (MQ=255)
                                                      aCGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAAc                            >  1:1853459/1‑61 (MQ=255)
                                                      aCGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAAc                            >  1:3098874/1‑61 (MQ=255)
                                                      aCGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTg                  >  1:855759/1‑71 (MQ=255)
                                                       cGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTgg                 <  1:4442729/71‑1 (MQ=255)
                                                         tCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAg                               <  1:1064637/55‑1 (MQ=255)
                                                         tCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAg                               <  1:1407141/55‑1 (MQ=255)
                                                         tCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTg                  <  1:5367911/68‑1 (MQ=255)
                                                           ggTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAAtt             <  1:4948393/71‑1 (MQ=255)
                                                           ggTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAAt              <  1:3516121/70‑1 (MQ=255)
                                                            gTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATc                     <  1:3583432/62‑1 (MQ=255)
                                                               cAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAg                               >  1:4526654/1‑49 (MQ=255)
                                                               cAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAAttt            >  1:2205639/1‑68 (MQ=255)
                                                                  gAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGa        >  1:1133195/1‑69 (MQ=255)
                                                                  gAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGAcc      >  1:5313212/1‑71 (MQ=255)
                                                                  gAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGAcc      >  1:2011459/1‑71 (MQ=255)
                                                                    aGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGt                                     >  1:3406862/1‑38 (MQ=255)
                                                                    aGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGt                                     >  1:1089521/1‑38 (MQ=255)
                                                                      ttACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGc   >  1:78193/1‑70 (MQ=255)
                                                                      ttACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCg  <  1:1157656/71‑1 (MQ=255)
                                                                      ttACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCg  <  1:1933110/71‑1 (MQ=255)
                                                                      |                                                                      
CGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCG  >  Exported/1439868‑1440008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: