| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,439,969 | G→A | 14.6% | Y111Y (TAC→TAT) | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,439,969 | 0 | G | A | 14.6% | 264.1 / 25.4 | 96 | Y111Y (TAC→TAT) | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base G (41/40); new base A (6/8); total (47/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01 | |||||||||||
GTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGT > Exported/1439900‑1440039 | gtCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCg > 1:1314500/1‑70 (MQ=255) gccaccaAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGt > 1:1029332/2‑71 (MQ=2) accaccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAg < 1:234209/70‑1 (MQ=255) caccGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTc > 1:3122079/1‑70 (MQ=255) caccAAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTc > 1:3933879/1‑70 (MQ=2) accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGa < 1:3548694/71‑1 (MQ=255) accGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGa < 1:1899483/71‑1 (MQ=255) accAAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCg > 1:2077768/1‑70 (MQ=2) accAAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGa < 1:2183233/71‑1 (MQ=2) accAAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGa < 1:3865498/71‑1 (MQ=2) accAAATTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGa < 1:3858876/71‑1 (MQ=2) caaaTTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGa > 1:2805084/3‑69 (MQ=2) gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGa > 1:1224023/1‑68 (MQ=255) gAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAg < 1:4759651/71‑1 (MQ=255) aaaTTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGa < 1:1639409/67‑1 (MQ=9) aaaTTCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGAAAg > 1:4231383/2‑71 (MQ=2) ttCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATa < 1:2916126/60‑1 (MQ=2) ttCTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATa < 1:1568840/60‑1 (MQ=2) cTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTa < 1:2263981/58‑1 (MQ=255) cTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTa < 1:1653329/58‑1 (MQ=255) cTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCCTa < 1:4663147/58‑1 (MQ=255) cTGGCAGCATATCGGTCCATGCTTCTACGTCATATACGACACCGTAGTTACGACCATAGTCGaaa > 1:3960016/1‑65 (MQ=2) gTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGa < 1:4038902/61‑1 (MQ=255) gTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATcc < 1:556987/71‑1 (MQ=255) tACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTATTTACGACCGTAGTCGAAAGAACCCACATcc < 1:5483669/70‑1 (MQ=255) tACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCg < 1:379425/55‑1 (MQ=255) tACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCg < 1:5182644/55‑1 (MQ=255) tACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGaa < 1:4545436/57‑1 (MQ=255) tACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGaa < 1:379437/57‑1 (MQ=255) tACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGaa < 1:748351/57‑1 (MQ=255) aCGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAAc > 1:3098874/1‑61 (MQ=255) aCGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAAc > 1:1853459/1‑61 (MQ=255) aCGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTg > 1:855759/1‑71 (MQ=255) cGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTgg < 1:4442729/71‑1 (MQ=255) cGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTg < 1:4490468/70‑1 (MQ=255) cGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTg < 1:1887784/70‑1 (MQ=255) tCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAg < 1:1407141/55‑1 (MQ=255) tCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAg < 1:1064637/55‑1 (MQ=255) tCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTg < 1:5367911/68‑1 (MQ=255) ggTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAAtt < 1:4948393/71‑1 (MQ=255) ggTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAAt < 1:3516121/70‑1 (MQ=255) ggTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAAt < 1:3756184/70‑1 (MQ=255) gTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATc < 1:3583432/62‑1 (MQ=255) gTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAAttt > 1:803002/1‑71 (MQ=255) cAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAg > 1:4526654/1‑49 (MQ=255) cAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAAttt > 1:1061861/1‑68 (MQ=255) cAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAAttt > 1:2205639/1‑68 (MQ=255) gAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGa > 1:1133195/1‑69 (MQ=255) gAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGAcc > 1:5313212/1‑71 (MQ=255) gAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGAcc > 1:2011459/1‑71 (MQ=255) aGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGt > 1:3406862/1‑38 (MQ=255) aGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGt > 1:1089521/1‑38 (MQ=255) aGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGaa > 1:4401152/1‑60 (MQ=255) ttACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGc > 1:78193/1‑70 (MQ=255) ttACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCg < 1:584078/71‑1 (MQ=255) ttACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCg < 1:1157656/71‑1 (MQ=255) ttACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCg < 1:1933110/71‑1 (MQ=255) ctACGTCATATACGACACCGTAGTTACGACCATAGTCGa < 1:5472907/38‑1 (MQ=17) ctACGTCATATACGACACCGTAGTTACGACCATAGTCGa < 1:107261/38‑1 (MQ=17) tACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCa > 1:3218599/1‑62 (MQ=255) aCGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAg < 1:2195251/40‑1 (MQ=255) aCGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGaa > 1:1402119/1‑42 (MQ=255) aCGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGaa > 1:806747/1‑42 (MQ=255) aCGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGa < 1:5567178/70‑1 (MQ=255) tAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGc > 1:5628671/1‑62 (MQ=255) tAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCca > 1:5311221/1‑70 (MQ=255) aaaCAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCcaca > 1:773917/1‑71 (MQ=255) aacaacGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAAt > 1:4470062/1‑64 (MQ=255) acaacGACGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGa > 1:735530/1‑54 (MQ=255) caacGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACgg > 1:1844581/1‑71 (MQ=255) caacGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACgg < 1:3226849/71‑1 (MQ=255) caacGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACgg < 1:2057451/71‑1 (MQ=255) gCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTcc < 1:72899/71‑1 (MQ=255) ccGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAAttt < 1:2193276/44‑1 (MQ=255) cGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAg < 1:1995693/71‑1 (MQ=255) cGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGGCCAg < 1:2002255/71‑1 (MQ=255) gTTACGACCGTAGTCGAAAGAAGCCACATCCTGGAATTTc > 1:1462831/1‑40 (MQ=255) gTTACGACCGTAGTCGAAAGAACCCACATCCTTGAATTTCAGACCTGCGAATGCCacac > 1:4641129/1‑59 (MQ=255) gTTACGACCGTAGTCGAAAGAACCCACATCCTGGaa > 1:985576/1‑36 (MQ=255) gTTACGACCGTAGTCGAAAGAACCCACATCCTGGaa > 1:4183890/1‑36 (MQ=255) gTTACGACCGTAGTCGAAAGAACCCACATCCTGGaa > 1:4154746/1‑36 (MQ=255) gTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTc > 1:1967222/1‑40 (MQ=255) gTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCt < 1:3095008/46‑1 (MQ=255) gTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCt < 1:2243771/46‑1 (MQ=255) gTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAgt > 1:3962824/1‑71 (MQ=255) ttACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGa < 1:3851435/42‑1 (MQ=255) aCGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGAc > 1:865864/1‑41 (MQ=255) aCGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGAc > 1:1098079/1‑41 (MQ=255) aCGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAgttgt > 1:3231190/1‑71 (MQ=255) aCGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAgttgt > 1:2263199/1‑71 (MQ=255) aCGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAgttgt > 1:1771934/1‑71 (MQ=255) aCGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAgtt > 1:810727/1‑69 (MQ=255) cGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGc < 1:2952794/44‑1 (MQ=255) cGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAgttgt < 1:1134603/70‑1 (MQ=255) gACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCca > 1:4494731/1‑51 (MQ=255) gACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCca > 1:5175015/1‑51 (MQ=255) ccGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACgg > 1:1495117/1‑54 (MQ=255) gTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTgttttcgt < 1:5010771/71‑1 (MQ=255) | GTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCAGGAGTTGTTTTCGT > Exported/1439900‑1440039 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |