| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,440,227 | G→A | 23.9% | Y25Y (TAC→TAT) | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,440,227 | 0 | G | A | 23.9% | 205.0 / 49.5 | 96 | Y25Y (TAC→TAT) | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base G (44/29); new base A (16/7); total (60/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 108 total aligned reads displayed.
TGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTT > Exported/1440160‑1440297 | tgttgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTa < 1:4519547/70‑1 (MQ=255)tgttgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTa > 1:2978238/1‑70 (MQ=255) gttgtCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTaa < 1:3901941/70‑1 (MQ=255) ttatCAGAAAAATAATGCAGGCCATCAACTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATaa > 1:4584766/4‑69 (MQ=2) ttatCAGAAAAATAATGCAGGCCATCAACTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATAAAc > 1:5075339/4‑71 (MQ=9) tCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACtt < 1:2968300/70‑1 (MQ=255) tCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACtt > 1:323894/1‑70 (MQ=255) agagAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTcag > 1:2106970/1‑71 (MQ=255) agagAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTcag > 1:4428153/1‑71 (MQ=255) gaAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTcagcag > 1:1661661/1‑71 (MQ=255) aaaaTAATGCAGGCCATCAACTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATAAACTTcggctg > 1:1430497/2‑69 (MQ=9) aaaTAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTc > 1:3737092/1‑65 (MQ=255) aGTGCAGGCCGTCTACTTTTCCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGttt > 1:1521748/1‑71 (MQ=255) cAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTca < 1:4235278/58‑1 (MQ=255) aGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtgctgc < 1:482148/71‑1 (MQ=255) ggCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTgcg < 1:767531/71‑1 (MQ=255) ggCCATCAACTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGtgcagca < 1:2463377/71‑2 (MQ=12) tCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTg < 1:3581500/70‑1 (MQ=255) tACTTTTCCGTACAAATCCAGTTTATTACCATCTTTATTATAGACTTCAGCCGCATTTGCTGCGCCAGc < 1:3016962/69‑1 (MQ=2) tACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTc > 1:2609717/1‑48 (MQ=255) tACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGttt < 1:3279653/58‑1 (MQ=255) tACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTa > 1:1528568/1‑71 (MQ=255) aaCTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATAAAc > 1:5362326/2‑45 (MQ=17) aaCTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATAAAc > 1:1861550/2‑45 (MQ=17) aaCTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATAAAc > 1:4446776/2‑45 (MQ=17) aaCTTTGCCATACAGATCTAATTTGTTGCCGTCTTTATTATAAAc > 1:1064332/2‑45 (MQ=17) aCTTTTCCGTACAAATCCAGTTTATTACCATCTTTATTATAGACTTCAGCCGCATTTGCTGCGcc > 1:165270/1‑65 (MQ=2) aCTTTTCCGTACAAATCCAGTTTATTACCATCTTTATTATAGACTTCAGCCGCATTTGCTGCGcc > 1:1285980/1‑65 (MQ=2) aCTTTTCCGTACAAATCCAGTTTATTACCATCTTTATTATAGACTTCAGCCGCATTTGCTGCGcc > 1:2294653/1‑65 (MQ=2) cTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTa < 1:4598928/69‑1 (MQ=255) tttACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTgcgc < 1:934179/62‑1 (MQ=255) tttACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTgcgc < 1:4836368/62‑1 (MQ=255) tttACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTAc > 1:4481721/1‑69 (MQ=255) tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag > 1:1937160/1‑70 (MQ=255) tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag > 1:1621446/1‑70 (MQ=255) tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag > 1:520484/1‑70 (MQ=255) tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag > 1:4389517/1‑70 (MQ=255) tACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag > 1:496206/1‑70 (MQ=255) ccatACAGATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTGCGGCgagcag < 1:3618084/68‑1 (MQ=255) tACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTcagca < 1:5490912/42‑1 (MQ=255) tACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTcagca < 1:1616235/42‑1 (MQ=255) tACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGt > 1:4747511/1‑46 (MQ=255) tACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtgct < 1:4509361/51‑1 (MQ=255) tACAGATCTAATTTGTT‑CCGTCTTTGTTGTAAACTTcagca < 1:4550452/41‑1 (MQ=39) aCAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACcag > 1:2338551/1‑64 (MQ=255) aCAGATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTGcggc < 1:1619161/61‑4 (MQ=255) aCAGATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTGCGGCgagcag < 1:235588/67‑1 (MQ=255) cAGATCTAATTTGTTGCCGTCTTTGTTGTAAACtt > 1:3135967/1‑35 (MQ=255) cAGATCTAATTTGTTGCCGTCTTTGTTGTAAACtt > 1:4638165/1‑35 (MQ=255) cAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTc > 1:4225050/1‑36 (MQ=255) cAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTc > 1:172728/1‑36 (MQ=255) cAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTc > 1:5527792/1‑36 (MQ=255) cAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTg < 1:1884941/71‑1 (MQ=255) aGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGttt < 1:3575862/45‑1 (MQ=255) aGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTgg > 1:1228954/1‑71 (MQ=255) aGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTgg > 1:4972598/1‑71 (MQ=255) gATCTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGtgcagc < 1:5338854/49‑1 (MQ=16) aTCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCTTTtgc > 1:4884715/1‑45 (MQ=255) aTCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtgc > 1:1788181/1‑45 (MQ=255) tCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGtt > 1:4738667/1‑41 (MQ=255) cTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTGCGGCGAgcagggcaggaat > 1:2648995/1‑67 (MQ=255) cTAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCTGCGGCGAgcagggcaggaat > 1:4935129/1‑67 (MQ=255) tAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGt > 1:439235/1‑38 (MQ=255) tAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGt > 1:1495279/1‑38 (MQ=255) tAATTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGTGCAGCACCt < 1:1763009/49‑1 (MQ=12) aaTTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTtg > 1:5116743/1‑40 (MQ=255) aaTTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCag > 1:2153372/1‑71 (MQ=255) aTTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGAc < 1:788333/67‑1 (MQ=255) aTTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGtgcagcac > 1:1627459/1‑43 (MQ=9) aTTTGTTGCCGTCTTTATTATAAACTTCGGCTGCATGtgcagca > 1:2573411/1‑43 (MQ=9) tttGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGAcc < 1:5335710/67‑1 (MQ=255) tttGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCagga < 1:3937048/71‑1 (MQ=255) tttGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCagga < 1:204270/71‑1 (MQ=255) tttGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCag > 1:2103598/1‑69 (MQ=255) tgttgCCGTCTTTGTTGTAAACTTCAGCAGCGTTtg > 1:2438350/1‑36 (MQ=255) tgttgCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCaggagg > 1:1721441/1‑71 (MQ=255) gttgCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCaggagg < 1:3760441/70‑1 (MQ=255) ccGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAg < 1:75376/71‑1 (MQ=255) gTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTa > 1:3093427/1‑71 (MQ=255) tCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGCGCTGGGACCAGGAGGGACAGTAc > 1:2184546/1‑71 (MQ=255) tCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGa < 1:2971903/55‑1 (MQ=255) tCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGAcc < 1:3814144/57‑1 (MQ=255) cTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTa < 1:4306071/39‑1 (MQ=255) tttGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCag < 1:4770638/57‑1 (MQ=255) tttGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACtt > 1:4708676/1‑71 (MQ=255) tttGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACtt > 1:2461263/1‑71 (MQ=255) tttGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACtt > 1:5492746/1‑71 (MQ=255) tttGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACtt > 1:3535897/1‑71 (MQ=255) tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGATCTGGGACCAGGAGGGACAGTACttt < 1:1410667/70‑1 (MQ=255) tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACttt > 1:3792894/1‑70 (MQ=255) tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACttt < 1:169379/70‑1 (MQ=255) tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACttt > 1:4949594/1‑70 (MQ=255) tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACttt > 1:3094092/1‑70 (MQ=255) tgttgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACtt < 1:3888863/69‑1 (MQ=255) tgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACca > 1:4931922/1‑36 (MQ=255) tgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACca > 1:3527272/1‑36 (MQ=255) tgtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACca > 1:1156736/1‑36 (MQ=255) gtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTAc > 1:3194175/1‑63 (MQ=255) gtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTAc > 1:4123593/1‑63 (MQ=255) gtAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACtt > 1:1963810/1‑71 (MQ=255) | TGTTGTCAGAGAAATAGTGCAGGCCGTCTACTTTACCGTACAGATCTAATTTGTTGCCGTCTTTGTTGTAAACTTCAGCAGCGTTTGCTGCGCCTGCTACCAGCAGAGCTGGGACCAGGAGGGACAGTACTTTAACTT > Exported/1440160‑1440297 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |