| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,474,821 | T→G | 26.7% | intergenic (+119/‑115) | nrdA → / → nrdB | ribonucleoside diphosphate reductase 1, alpha subunit/ribonucleoside diphosphate reductase 1, beta subunit, ferritin‑like |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,474,821 | 0 | T | G | 26.7% | 58.6 / 19.6 | 30 | intergenic (+119/‑115) | nrdA/nrdB | ribonucleoside diphosphate reductase 1, alpha subunit/ribonucleoside diphosphate reductase 1, beta subunit, ferritin‑like |
| Reads supporting (aligned to +/- strand): ref base T (16/6); new base G (6/2); total (22/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.32e-02 | |||||||||||
GTTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACGGCTCGGA‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCG > Exported/1474752‑1474889 | gTTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTa < 1:1571196/71‑1 (MQ=255)gTTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTa < 1:3915434/71‑1 (MQ=255)gTTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTa < 1:2315959/71‑1 (MQ=255) gcgcCAGCGTCGCATCAGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATcc > 1:2005419/1‑54 (MQ=255) gcgcCAGCGTCGCATCAGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACGGCTCGGA‑tt < 1:3803575/71‑1 (MQ=255) cAGCGTCGCATCAGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACGGCTCGGA‑TTTg > 1:5391049/1‑69 (MQ=255) gCATCAGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACGGCTCGGA‑TTTGTAGGCCTGa < 1:5172349/71‑1 (MQ=255) cAGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACGGCTCGGA‑TTTGTAGGCCTGAt > 1:4350047/1‑68 (MQ=255) aGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTAt > 1:4150126/1‑36 (MQ=255) aGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTAc > 1:106070/1‑45 (MQ=255) aGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACGGCTCGGA‑TTTGTAGGCCTGATAAGa > 1:1587807/1‑71 (MQ=255) cGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACGGCTCGGA‑TTTGTAGGCCTGATAAGACgcgc > 1:2051645/1‑70 (MQ=255) tgTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAACGGTTCGGATTTTGTAGGCCTGATAAGAcgcgg > 1:1857138/2‑68 (MQ=2) tgTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAACGGTTCGGATTTTGTAGGCCTGATAAGAcgcgg > 1:4615802/2‑68 (MQ=2) gTGCTGGACGTAGCGTAAACGCCTGATCCGTCCAACGGTTCGGATTTTGTAGGCCTGATAAGAcgcggt < 1:2532356/69‑3 (MQ=2) gATGCAGCGTGAACGCCTTATCCGGCCTACGGCTCGGA‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCa > 1:77001/1‑71 (MQ=255) gATGCAGCGTGAACGCCTTATCCGGCCTACGGCTCGGA‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCa > 1:5542865/1‑71 (MQ=255) aTGCAGCGTGAACGCCTTATCCGGCCTACGGCTCGGA‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCAt > 1:661098/1‑71 (MQ=255) cAGCGTGAACGCCTTATCCGGCCTACGGCTCGGA‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAg > 1:2206749/1‑71 (MQ=255) aGCGTGAACGCCTTATCCGGCCTACGGCTCGGA‑tt > 1:5494721/1‑35 (MQ=255) aGCGTGAACGCCTTATCCGGCCTACGGCTCGGA‑tt > 1:922637/1‑35 (MQ=255) aGCGTAAACGCCTGATCCGTCCAACGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCAt > 1:5051680/1‑68 (MQ=2) taaaCGCCTGATCCGTCCAACGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTc > 1:3925360/3‑60 (MQ=2) taaaCGCCTGATCCGTCCAACGGTTCGGATTTTGTAGGCCTGATAAGACGCGGTAGCGTCGCATCAGGcat < 1:5142320/69‑2 (MQ=2) gAACGCCTTATCCGGCCTACGGCTCGGA‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAg > 1:5173600/1‑71 (MQ=255) aaCGCCTGATCCGTCCAACGGTTCGGATTTTGTAGGCCTGATAAGa > 1:431275/1‑46 (MQ=9) aaCGCCTGATCCGTCCAACGGTTCGGATTTTGTAGGCCTGATAAGAcgcg > 1:4923270/1‑50 (MQ=9) gCCTTATCCGGCCTACGGCTCGGA‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATg > 1:1205345/1‑71 (MQ=255) ccTTATCCGGCCTACGGCTCGGA‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATgc > 1:5169314/1‑71 (MQ=255) cTTATCCGGCCTACGGCTCGGA‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATgcg < 1:2114059/71‑1 (MQ=255) tATCCGGCCTACGGCTCGGA‑TTTGTAGGCCTGATAAGACGCGCCAGCg < 1:2806915/48‑1 (MQ=255) | GTTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCTCCGGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACGGCTCGGA‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCG > Exported/1474752‑1474889 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |