| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,502,353 | G→A | 71.4% | S92L (TCA→TTA) | lrhA ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,502,353 | 0 | G | A | 71.4% | 100.0 / 53.0 | 77 | S92L (TCA→TTA) | lrhA | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base G (10/12); new base A (23/32); total (33/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.92e-01 | |||||||||||
GGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTT > Exported/1502284‑1502422 | ggTAAGATCGTATCGGCAGATTCATCTGAAGCAACGATAGTTAATACGCCCTGAAGATTACTGAACATtga < 1:2239079/71‑1 (MQ=255) gTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATtaat < 1:2825380/71‑1 (MQ=255) gTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATtaat < 1:4962868/71‑1 (MQ=255) aaGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATtaatga > 1:4263170/1‑71 (MQ=255) aaGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATtaatg < 1:2891828/70‑1 (MQ=255) aGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATtgatgat > 1:1772087/1‑71 (MQ=255) aGATCGTATCGGCAGATTCATCTGAAGAACCGATAGTTAATACGCCCTGAAGATTACTGAACATtaatgat < 1:2336477/71‑1 (MQ=255) tCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAg < 1:4113028/71‑1 (MQ=255) tCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAg < 1:5419225/71‑1 (MQ=255) ggCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATtaatgatga < 1:1108886/63‑1 (MQ=255) aGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTGATGATGAg < 1:5216287/61‑1 (MQ=255) aGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTGATGATGAGCAGGCCtcat < 1:2177312/71‑1 (MQ=255) aGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCtcat > 1:3739483/1‑71 (MQ=255) aTTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCtcatca > 1:2695602/1‑71 (MQ=255) ttCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTGATGATGAGCAGGCCtcat > 1:1285252/1‑68 (MQ=255) ttCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAg < 1:913042/58‑1 (MQ=255) ttCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTcatcat < 1:1541455/71‑1 (MQ=255) cATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCa > 1:1688282/1‑58 (MQ=255) aTCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTaa < 1:4456012/71‑1 (MQ=255) aTCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTaa < 1:5603238/71‑1 (MQ=255) cTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTaaa > 1:2275021/1‑70 (MQ=255) gAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACg > 1:4304129/1‑71 (MQ=255) aaGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACg < 1:101116/70‑1 (MQ=255) aaGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGc < 1:2606949/71‑1 (MQ=255) aGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGc < 1:4267768/70‑1 (MQ=255) gCACCGATAGTTAATACGCCCTGAAGATTACTGAACATtaatg < 1:1739875/43‑1 (MQ=255) gCACCGATAGTTAATACGCCCTGAAGATTACTGAACATtaatg < 1:5574860/43‑1 (MQ=255) gCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAg < 1:3999934/71‑1 (MQ=255) gCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAg < 1:2248706/71‑1 (MQ=255) gCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAg < 1:1399680/71‑1 (MQ=255) ccGATAGTTAATACGCCCTGAAGATTACTGAACATtaat < 1:2176617/39‑1 (MQ=255) ccGATAGTTAATACGCCCTGAAGATTACTGAACATtaat < 1:4310066/39‑1 (MQ=255) ccGATAGTTAATACGCCCTGAAGATTACTGAACATtaat < 1:3421265/39‑1 (MQ=255) ccGATAGTTAATACGCCCTGAAGATTACTGAACATtaat < 1:2385389/39‑1 (MQ=255) ccGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGAt > 1:1851056/1‑71 (MQ=255) gATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGAttt > 1:611902/1‑71 (MQ=255) gATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGAttt > 1:1075667/1‑71 (MQ=255) aTAGTTAATACGCCCTGAAGATTACTGAACATTGATGATGAGCAGGCCTCATCATTaaaa > 1:4494701/1‑60 (MQ=255) tAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTc < 1:3549175/71‑1 (MQ=255) tAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTc < 1:1450404/71‑1 (MQ=255) tAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTc < 1:2413594/71‑1 (MQ=255) caGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTc < 1:5470292/70‑1 (MQ=255) aGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTcc > 1:4058042/1‑71 (MQ=255) gTTAATACGCCCTGAAGATTACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCt > 1:4879952/1‑71 (MQ=255) gTTAATACGCCCTGAAGATTACTGAACATTAATGATGAg < 1:5471502/39‑1 (MQ=255) gTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGAttt < 1:177308/67‑1 (MQ=255) gTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTc < 1:220712/69‑1 (MQ=255) ttAATACGCCCTGAAGATTACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACg < 1:4429584/58‑1 (MQ=255) ttAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTg < 1:2008972/71‑1 (MQ=255) tAATACGCCCTGAAGATTACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTgg > 1:4727244/1‑71 (MQ=255) tAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTc > 1:3737608/1‑67 (MQ=255) aaTACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTaaaa > 1:1750752/1‑54 (MQ=255) aaTACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGc > 1:5436085/1‑71 (MQ=255) aTACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGc < 1:1560820/70‑1 (MQ=255) tccGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGt < 1:5568086/69‑1 (MQ=255) tACGCCCTGAAGATTACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGc > 1:3688278/1‑69 (MQ=255) tACGCCCTGAAGATTACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGt < 1:4783014/71‑1 (MQ=255) tACGCCCTGAAGATTACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGt < 1:4294762/71‑1 (MQ=255) tACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGt < 1:5537475/71‑1 (MQ=255) cGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCtcat > 1:2543261/1‑42 (MQ=255) cGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCtcat > 1:1873940/1‑42 (MQ=255) cccTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAAcc < 1:4670237/71‑1 (MQ=255) cccTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAAc > 1:1163387/1‑70 (MQ=255) ccTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCa < 1:2595546/71‑1 (MQ=255) cTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCaa < 1:3256079/71‑1 (MQ=255) tGAAGATTACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTc > 1:3805042/1‑57 (MQ=255) tGAAGATTACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTc < 1:3437739/57‑1 (MQ=255) gAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCaag > 1:1582250/1‑70 (MQ=255) gAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCaag > 1:4151803/1‑70 (MQ=255) gAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCaag > 1:5184145/1‑70 (MQ=255) aaGATTACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCaag > 1:5235232/1‑69 (MQ=255) aaGATTACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCaag < 1:5463919/69‑1 (MQ=255) aaGATTACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCaa > 1:5633392/1‑68 (MQ=255) aaGATTACTGAACATTAATGATGAGCAGTCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCaag < 1:3271006/69‑1 (MQ=255) aaGATTACTGAACATTAATGATGAGCAGGCCTCATCATTaaaa > 1:1096558/1‑43 (MQ=255) aaGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCaag > 1:1960009/1‑69 (MQ=255) aaGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCaag > 1:2812461/1‑69 (MQ=255) aaGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCaag < 1:23455/69‑1 (MQ=255) aaGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCaag < 1:230341/69‑1 (MQ=255) gATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGt > 1:363608/1‑71 (MQ=255) aTTACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTg < 1:1662675/55‑1 (MQ=255) aTTACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTg < 1:1118888/55‑1 (MQ=255) aTTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGt < 1:699055/59‑1 (MQ=255) aTTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGtt < 1:1987069/71‑1 (MQ=255) ttACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTg < 1:3307326/71‑1 (MQ=255) tACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGc > 1:2519391/1‑55 (MQ=255) tACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCaagaa > 1:371009/1‑66 (MQ=255) tACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTaa > 1:2747789/1‑59 (MQ=255) aCTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGAttt < 1:3884267/47‑1 (MQ=255) aTTGATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTg < 1:4127021/62‑1 (MQ=255) aTTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTg < 1:2687156/62‑1 (MQ=255) tgatgatgaGCAGGCCTCATCATTAAAACGCAGGATTTTTCTGGCGTAACCAAGAAGTTGAATGCCATGtt > 1:571877/1‑71 (MQ=255) | GGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTGATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTT > Exported/1502284‑1502422 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |