| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,591,658 | C→T | 11.4% | intergenic (+117/+72) | yfeS → / ← cysM | conserved hypothetical protein/cysteine synthase B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,591,658 | 0 | C | T | 11.4% | 170.7 / 9.5 | 61 | intergenic (+117/+72) | yfeS/cysM | conserved hypothetical protein/cysteine synthase B |
| Reads supporting (aligned to +/- strand): ref base C (30/24); new base T (3/4); total (33/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01 | |||||||||||
TATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGTCATCAGTCTCCGATGCTAT > Exported/1591590‑1591723 | tATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAAc > 1:2781268/1‑69 (MQ=255)tATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCt > 1:2820729/1‑71 (MQ=255) gATGCGGCGTAAACGCCTTATCCGGCCTACAAATTCTTCCAAATTCAATATATTGCAGAAATCATGTAGGc > 1:2859712/1‑71 (MQ=11) gATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAgg > 1:161687/1‑70 (MQ=255) gATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGc < 1:487477/71‑1 (MQ=255) gATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGc > 1:3033291/1‑71 (MQ=255) tGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCt < 1:4258487/71‑1 (MQ=255) gcggcgTAAACGCCTTATCCGGCCTACAAATTCTTCCAAATTCAATATATTGCAGAAATCATGTa > 1:4840718/1‑65 (MQ=11) cggcgTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAAc > 1:5689638/1‑58 (MQ=255) cttAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATaa < 1:1974287/69‑1 (MQ=255) gTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAg < 1:291041/71‑1 (MQ=255) gTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAg > 1:1172367/1‑71 (MQ=255) tAAACGCCTTATCCGGCCTACAAATTCTTCCAAATTCAATATATTGCAGAAATCATGTAg < 1:1589502/60‑1 (MQ=11) tAAACGCCTTATCCGGCCTACAAATTCTTCCAAATTCAATATATTGCAGAAATCATGTAGGCCt < 1:3975788/64‑1 (MQ=11) tAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAACCCTTGt < 1:3206/58‑1 (MQ=255) tAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAg < 1:579092/70‑1 (MQ=255) aaCGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCg > 1:3680747/1‑70 (MQ=255) aaCGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCg > 1:3221703/1‑70 (MQ=255) aCGCCTTATCCGGCCTACAAATTCTTCCAAATTCAATATATTGCAGAAATCATGTAGGCCTGATAAGCGTa < 1:2269605/71‑1 (MQ=11) gCCTTATCCGGCCTACAAATTCTTCCAAATTCAATATATTGCAGAAATCATGTAGGCCTGATAAGCGTAgc > 1:2944531/1‑71 (MQ=11) cTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAgcg > 1:234498/1‑70 (MQ=255) attATCCGGCCTACAAAATCGTGCTAATTCAATATATTGGAGAAACCTTg < 1:4279856/49‑1 (MQ=255) tATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTa > 1:3572312/1‑50 (MQ=255) tATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCAt > 1:1372159/1‑71 (MQ=255) tATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCAt > 1:1725128/1‑71 (MQ=255) tATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCAt > 1:1676644/1‑71 (MQ=255) aTCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCtt < 1:136632/46‑1 (MQ=255) aTCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCAt > 1:1384668/1‑70 (MQ=255) aTCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCAt > 1:596644/1‑70 (MQ=255) tCCGGCCTACAAATTCTTCCAAATTCAATATATTGCAGAAATCATGTAGGCCTGATAAGCGTAGCGCATCa < 1:4161649/71‑1 (MQ=11) tACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTg < 1:559899/47‑1 (MQ=255) tACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAgcgc < 1:5312983/60‑1 (MQ=255) cAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATaa > 1:5266805/1‑49 (MQ=255) cAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCa > 1:2144260/1‑59 (MQ=255) aaaTCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGtt > 1:4591587/1‑67 (MQ=255) aaTCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCg > 1:5019737/1‑49 (MQ=255) aaTCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCg > 1:5153723/1‑71 (MQ=255) aTCGTGCTAATTCAATATATTGCAGAAACCTTGTa > 1:2087793/1‑35 (MQ=255) tGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGt < 1:5566025/71‑1 (MQ=255) tGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGt < 1:61967/71‑1 (MQ=255) cTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGtca < 1:2307079/71‑1 (MQ=255) cTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGtca < 1:1215325/71‑1 (MQ=255) cTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGtc < 1:1925143/70‑1 (MQ=255) tAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGttt > 1:3445288/1‑59 (MQ=255) tAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGttt > 1:3941392/1‑59 (MQ=255) aTTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAg > 1:5526055/1‑42 (MQ=255) aTTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGtcat < 1:5241376/69‑1 (MQ=255) aTTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGtcat < 1:5247975/69‑1 (MQ=255) tCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGtttt < 1:664481/56‑1 (MQ=255) cAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTa > 1:3956066/1‑38 (MQ=255) cAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGtttt > 1:324939/1‑55 (MQ=255) cAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGttt > 1:3762801/1‑54 (MQ=255) cAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGtcat > 1:324839/1‑66 (MQ=255) atatTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGt < 1:1526085/59‑1 (MQ=255) atatTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCACATCAGGCAGTTTTGCGTTTGt < 1:672142/59‑1 (MQ=255) tatTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGtcatca > 1:2110136/1‑63 (MQ=255) tatTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGtcatca > 1:4745054/1‑63 (MQ=255) ttGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCATCa > 1:813325/1‑39 (MQ=255) tGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGt < 1:2280961/55‑1 (MQ=255) tGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGt < 1:3012577/55‑1 (MQ=255) aGAAACCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGTCATCAGTCTCCGATGCTAt < 1:87188/71‑1 (MQ=255) aGAAACCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGTCATCAGTCTCCGATGCTAt < 1:5002906/71‑1 (MQ=255) gAAACCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGTCATCAGTCTcc > 1:2853965/1‑62 (MQ=255) | TATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCTAATTCAATATATTGCAGAAACCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGTCATCAGTCTCCGATGCTAT > Exported/1591590‑1591723 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |