Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,614,557 G→C 9.4% intergenic (‑182/+35) eutH ← / ← eutG predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,614,5570GC 9.4% 184.6 / 7.7 64intergenic (‑182/+35)eutH/eutGpredicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization
Reads supporting (aligned to +/- strand):  ref base G (33/25);  new base C (4/2);  total (37/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTC  >  Exported/1614488‑1614627
                                                                     |                                                                      
gCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGc                                                                       >  1:3780197/1‑71 (MQ=255)
 cAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCt                                                                      >  1:4676485/1‑71 (MQ=255)
     tGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGa                                                                   >  1:3225964/1‑70 (MQ=18)
     tGCACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGa                                                                   >  1:3435211/1‑70 (MQ=18)
     tGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGa                                                                   >  1:2094292/1‑70 (MQ=255)
     tGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGa                                                                   >  1:4208425/1‑70 (MQ=255)
       cACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATaa                                                                <  1:38485/71‑1 (MQ=17)
       cACCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATa                                                                 >  1:4353166/1‑70 (MQ=17)
       cACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGTGTTCCCGTAGGTCGGAt                                                                  <  1:2887924/69‑1 (MQ=17)
       cACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATaa                                                                <  1:134099/71‑1 (MQ=255)
       cACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATACCGTAGGCTGGATaa                                                                <  1:3377068/71‑1 (MQ=255)
        aCCGCGCCACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATaaa                                                               <  1:2166123/71‑2 (MQ=17)
           gcgcCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATg                                                            <  1:2684412/71‑1 (MQ=255)
              ccACTGGCGGATGCGGCGTGGACGCCTTATCCGCCCTACATGTGCAATTCCGTAACCTGGATAAAAcgcga                                                         >  1:2501852/1‑70 (MQ=11)
                aCTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGa                                                              >  1:4402637/1‑64 (MQ=39)
                aCTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGa                                                              >  1:5423361/1‑64 (MQ=39)
                 cTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATg                                                            >  1:694400/1‑65 (MQ=40)
                  tGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGAt                                                                  <  1:3422101/58‑1 (MQ=35)
                   ggCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGAt                                                                  >  1:4950249/1‑57 (MQ=35)
                    gCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCg                                                   <  1:3243313/71‑1 (MQ=39)
                       gATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATaa                                                                <  1:1443038/55‑1 (MQ=35)
                         tGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCa                                              >  1:1219639/1‑71 (MQ=39)
                         tGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCa                                              >  1:1135395/1‑71 (MQ=39)
                             gcgTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATcct                                          <  1:2760224/71‑2 (MQ=39)
                             gcgTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCg                                          <  1:1312082/71‑1 (MQ=39)
                             gcgTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCg                                          <  1:1188101/71‑1 (MQ=39)
                              cgTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCgg                                         >  1:3390797/1‑71 (MQ=39)
                                 gAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAt                                      >  1:5103668/1‑71 (MQ=38)
                                    cGCCTTATCCGCCCTACATTTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTa                                   >  1:358416/1‑71 (MQ=255)
                                    cGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTa                                   <  1:4614628/71‑1 (MQ=40)
                                     gCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTAt                                  <  1:2353812/71‑1 (MQ=255)
                                      ccTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTAtt                                 >  1:777344/1‑71 (MQ=255)
                                        ttATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCa                                       <  1:3886567/63‑1 (MQ=35)
                                        ttATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAtt                                     <  1:1191221/65‑1 (MQ=39)
                                        ttATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAtt                                     <  1:1659852/65‑1 (MQ=39)
                                          aTCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCg                                                   <  1:5028668/49‑1 (MQ=38)
                                          aTCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAtt                                     <  1:3872988/63‑1 (MQ=39)
                                          aTCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAtt                                     <  1:2396444/63‑1 (MQ=39)
                                          aTCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTgcgc                             >  1:372983/1‑71 (MQ=255)
                                           tCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGcgcc                            <  1:300626/71‑1 (MQ=255)
                                             cGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGt                                                  >  1:5454511/1‑47 (MQ=38)
                                              gCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCt                         <  1:1043527/71‑1 (MQ=255)
                                              gCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCt                         <  1:1097927/71‑1 (MQ=255)
                                                 cTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAtt                                     <  1:1699595/56‑1 (MQ=40)
                                                 cTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAtt                                     <  1:2772147/56‑1 (MQ=40)
                                                 cTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAtt                                     <  1:2940960/56‑1 (MQ=40)
                                                  tACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGc                                        >  1:930632/1‑52 (MQ=35)
                                                  tACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCg                      >  1:2958133/1‑70 (MQ=255)
                                                   aCATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCAt                                             >  1:4692385/1‑46 (MQ=38)
                                                   aCATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTa                    >  1:384124/1‑71 (MQ=255)
                                                    cATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAttt                                    >  1:4106416/1‑54 (MQ=255)
                                                             tCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACa          <  1:1823027/71‑1 (MQ=255)
                                                               ccGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAAt        >  1:1072882/1‑71 (MQ=255)
                                                               ccGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAAt        >  1:2763398/1‑71 (MQ=255)
                                                               ccGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAAt        >  1:1153492/1‑71 (MQ=255)
                                                               ccGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAAt        >  1:1146633/1‑71 (MQ=255)
                                                                 gTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCt      >  1:1877804/1‑71 (MQ=255)
                                                                  tAGGCTGGATAAGATGCGGCAAGCGTCGCATCCTGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTg     >  1:1671148/1‑71 (MQ=255)
                                                                  tAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTg                        >  1:4987913/1‑52 (MQ=255)
                                                                  tAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCt      >  1:3973118/1‑70 (MQ=255)
                                                                  tAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTg     >  1:4648337/1‑71 (MQ=255)
                                                                  tAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTg     >  1:5225213/1‑71 (MQ=255)
                                                                  tAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTg     >  1:5400489/1‑71 (MQ=255)
                                                                  tAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTg     >  1:758710/1‑71 (MQ=255)
                                                                    ggCTGGATAAGATGCGGCAAGCGTCGCATCCGGCAtt                                     <  1:2583690/37‑1 (MQ=255)
                                                                    ggCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCt   >  1:3356269/1‑71 (MQ=255)
                                                                     gCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACa          <  1:1625991/63‑1 (MQ=255)
                                                                     gCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTc  <  1:4437047/71‑1 (MQ=255)
                                                                     gCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTc  <  1:4766487/71‑1 (MQ=255)
                                                                     |                                                                      
GCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGATGCGGCAAGCGTCGCATCCGGCATTTATTGCGCCGCTGCGTACAGGCCGACAATCTGCTC  >  Exported/1614488‑1614627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: