Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 309,843 C→T 25.7% G1010G (GGG→GGA acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported309,8430CT25.7% 118.8 / 39.5 70G1010G (GGG→GGAacrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base C (25/27);  new base T (9/9);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGG  >  Exported/309774‑309911
                                                                     |                                                                    
aaaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATcc                                                                     >  1:3255651/1‑71 (MQ=255)
 aaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATc                                                                      <  1:3234260/69‑1 (MQ=255)
 aaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATc                                                                      <  1:3879147/69‑1 (MQ=255)
 aaGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATc                                                                      <  1:2545637/69‑1 (MQ=255)
  aGCGGCGGCGAACCAACACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCg                                                                   >  1:3422099/1‑71 (MQ=255)
    cggcggcgAACCACCACAAAGAATACCGGAACGAAGAAGATTCCAGTACCGTTGCGGTCACCATcc                                                                     >  1:4777737/1‑66 (MQ=255)
     ggcggcgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGcc                                                                 <  1:3265306/70‑1 (MQ=255)
          cgAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTa                                                            <  1:4164197/70‑1 (MQ=255)
             accaccACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACAcc                                                        >  1:4442604/1‑71 (MQ=255)
              tcaccacAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTAcccc                                                        >  1:314986/2‑70 (MQ=255)
              ccaccaCAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCa                                                               >  1:5409611/1‑63 (MQ=255)
                accacAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGcc                                                                 <  1:3281504/59‑1 (MQ=255)
                accacAAAGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTaccc                                                         >  1:5354213/1‑65 (MQ=16)
                  cacaAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGcc                                                                 <  1:1440765/57‑1 (MQ=255)
                  cacaAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCa                                                               >  1:4718558/1‑59 (MQ=255)
                  cacaAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTAc                                                   >  1:4126667/1‑71 (MQ=255)
                   acaAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACCCCGGTAcc                                                  >  1:5554540/1‑71 (MQ=255)
                      aaGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTa                                                >  1:5162098/1‑70 (MQ=255)
                      aaGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATAccca                                                >  1:849491/1‑68 (MQ=12)
                       aGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTAcc                                                  <  1:182003/67‑1 (MQ=255)
                       aGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTAcc                                                  <  1:625993/67‑1 (MQ=255)
                       aGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTAcc                                                  <  1:847248/67‑1 (MQ=255)
                       aGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATtcctcc                                                                 <  1:973145/52‑1 (MQ=12)
                       aGAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATtcctcc                                                                 <  1:31127/52‑1 (MQ=12)
                        gAACACCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCAcag                                             >  1:2923753/1‑69 (MQ=255)
                          acaCCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCg                                           >  1:4712527/3‑71 (MQ=255)
                          acaCCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCg                                           >  1:3298615/3‑71 (MQ=255)
                           tACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTa                                                >  1:5670355/1‑65 (MQ=255)
                           tACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTa                                                >  1:2475731/1‑65 (MQ=255)
                            aCCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt                                         <  1:5275557/71‑1 (MQ=255)
                             ccGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTac                                                           <  1:878289/52‑1 (MQ=255)
                              cGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATAccca                                                >  1:5372972/1‑60 (MQ=255)
                               ggAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTac                                                           <  1:5521459/50‑1 (MQ=255)
                               ggAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTg                                      >  1:309971/1‑71 (MQ=255)
                               ggAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTg                                      <  1:2365160/71‑1 (MQ=255)
                                gtaCGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgc                                     <  1:2910880/69‑1 (MQ=255)
                                gtaCGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgc                                     <  1:2736173/69‑1 (MQ=255)
                                gAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgc                                     <  1:2729606/71‑1 (MQ=255)
                                 aaCGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTacac                                                         >  1:5452146/1‑50 (MQ=255)
                                 aaCGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTacac                                                         >  1:3542944/1‑50 (MQ=255)
                                  aCGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgc                                   >  1:2623255/1‑71 (MQ=255)
                                   cGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgcg                                  >  1:2352911/1‑71 (MQ=255)
                                   cGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt                                         >  1:487488/1‑64 (MQ=255)
                                   cGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt                                         >  1:1336105/1‑64 (MQ=255)
                                    gaagaaGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCAt                                                              <  1:5150395/42‑1 (MQ=255)
                                    gaagaaGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgcgc                                 <  1:4858816/71‑1 (MQ=255)
                                    gaagaaGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgcgcgc                                 <  1:1760126/71‑1 (MQ=12)
                                     aagaagATTGCCAGTACCGTTGCGGTCACCATCCCGCCCAt                                                              <  1:3118294/41‑1 (MQ=255)
                                     aagaagATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt                                         <  1:1588986/62‑1 (MQ=255)
                                     aagaagATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt                                         <  1:4603109/62‑1 (MQ=255)
                                     aagaagATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt                                         <  1:2606199/62‑1 (MQ=255)
                                       gaagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCgg                              <  1:1306413/71‑1 (MQ=255)
                                       gaagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCgg                              <  1:1517037/71‑1 (MQ=255)
                                        aagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCATTCTTCGCGCCGGa                             <  1:448472/71‑1 (MQ=255)
                                          gaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAAc                           >  1:3420077/1‑71 (MQ=255)
                                          gaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAAc                           >  1:3216696/1‑71 (MQ=255)
                                          gaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAAc                           <  1:91297/71‑1 (MQ=255)
                                           aTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAAc                           <  1:2801117/70‑1 (MQ=255)
                                           aTTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgcgcg                                  <  1:4069408/63‑1 (MQ=255)
                                           aTTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgcgcg                                  <  1:4018313/63‑1 (MQ=255)
                                              gCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAg                        >  1:4999943/1‑70 (MQ=255)
                                                   tACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAg                  >  1:1582566/1‑71 (MQ=255)
                                                    aCCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAg                  >  1:5516986/1‑70 (MQ=255)
                                                     ccGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTa                >  1:2665121/1‑71 (MQ=255)
                                                       gTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACt              >  1:1012766/1‑71 (MQ=255)
                                                        ttGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGGGTTCTGCGCGCCGGAACCAGCACCAGTACt              >  1:4175240/1‑70 (MQ=255)
                                                           cGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGAt           <  1:468344/70‑1 (MQ=255)
                                                             gTCACCATCCCGCCCATTACACCGGTACCTACTGCGt                                          <  1:5169313/37‑1 (MQ=255)
                                                             gTCACCATCCCGCCCATTACACCGGTACCTACTGCGt                                          <  1:4097617/37‑1 (MQ=255)
                                                             gTCACCATCCCGCCCATTACACCGGTACCTACTGCGt                                          <  1:4352798/37‑1 (MQ=255)
                                                             gTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATaa         <  1:2850347/70‑1 (MQ=255)
                                                              tCACCATCCCGCCCATTACACCGGTACCTACTGCg                                           <  1:719907/35‑1 (MQ=255)
                                                                aCCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAg     <  1:2622107/71‑1 (MQ=255)
                                                                  cATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAAc        >  1:1679130/1‑66 (MQ=255)
                                                                    tCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGa                             <  1:1986799/43‑1 (MQ=255)
                                                                    tCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTg             >  1:2983748/1‑59 (MQ=255)
                                                                    tCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCgg  >  1:2489622/1‑70 (MQ=255)
                                                                     |                                                                    
AAAGCGGCGGCGAACCACCACAAAGAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGG  >  Exported/309774‑309911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: