| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,639,265 | A→C | 41.8% | intergenic (+35/+75) | ypfN → / ← ypfH | hypothetical protein/predicted hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,639,265 | 0 | A | C | 41.8% | 15.5 / 103.7 | 83 | intergenic (+35/+75) | ypfN/ypfH | hypothetical protein/predicted hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (16/32); new base C (13/21); total (30/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTG > Exported/1639197‑1639335 | cgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGATTGCTCAACCGTAGGCCGGATAAGGCGTTaa > 1:1610808/1‑70 (MQ=255)cgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAAc < 1:5109494/71‑1 (MQ=255) gacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAAcg > 1:1341978/1‑71 (MQ=255) gacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAAcg > 1:162503/1‑71 (MQ=255) cTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAAcgcc > 1:5240235/1‑71 (MQ=255) cTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAAcgcc > 1:2138618/1‑71 (MQ=255) ccGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCAt < 1:2223954/71‑1 (MQ=255) gAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATcc > 1:4687786/1‑71 (MQ=255) aaaaGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTaa > 1:3273196/1‑59 (MQ=255) aaaaGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTaa > 1:3526198/1‑59 (MQ=255) aaaGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGc < 1:4801720/71‑1 (MQ=255) aaGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGc > 1:5086789/1‑70 (MQ=255) aGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTaa > 1:2707513/1‑56 (MQ=255) aGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTaa > 1:3242584/1‑56 (MQ=255) aGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTaa > 1:1604122/1‑56 (MQ=255) aGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCaa < 1:1590836/71‑1 (MQ=255) aGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCaa < 1:1008489/71‑1 (MQ=255) gATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGc < 1:5488717/67‑1 (MQ=255) aTCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATgg > 1:4104017/1‑71 (MQ=255) aaCCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTaa > 1:3936121/1‑50 (MQ=255) aaCCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTaa > 1:445781/1‑50 (MQ=255) aaCCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTaa > 1:4401746/1‑50 (MQ=255) aaCCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTaa > 1:1166444/1‑50 (MQ=255) aaCCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTg < 1:1121924/70‑1 (MQ=255) ccGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAAc < 1:3442558/71‑1 (MQ=255) ccGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAAc > 1:4502322/1‑71 (MQ=255) ccGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAAc < 1:3704294/71‑1 (MQ=255) cGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAAc < 1:773098/70‑1 (MQ=255) gaagaaGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGa > 1:511664/1‑71 (MQ=255) gaagTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGc < 1:2470358/71‑1 (MQ=255) gTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTaa > 1:431434/1‑40 (MQ=255) gTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCa < 1:3339986/47‑1 (MQ=255) gTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCa < 1:1163753/47‑1 (MQ=255) gTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCt > 1:841144/1‑70 (MQ=255) tAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGAGTTAAcg < 1:2334225/41‑1 (MQ=255) aaGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGAt > 1:4334209/1‑71 (MQ=255) gCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGc < 1:2063037/71‑1 (MQ=255) tGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGa > 1:217416/1‑71 (MQ=255) aGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGc < 1:2380901/45‑1 (MQ=255) aGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGa < 1:1472496/69‑1 (MQ=255) aGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACg < 1:176796/71‑1 (MQ=255) aGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACg < 1:170100/71‑1 (MQ=255) gTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGc < 1:754492/71‑1 (MQ=255) tGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCt < 1:3028274/71‑1 (MQ=255) attcgtaGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgctacgcttat > 1:2786583‑M1/4‑46 (MQ=255) attcgtaGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgctacgcttat < 1:4143815‑M1/68‑26 (MQ=255) attcgtaGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgctacgcttat < 1:2626015‑M1/68‑26 (MQ=255) attcgtaGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgctacgcttat > 1:1694301‑M1/4‑46 (MQ=255) actcgtaGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgctacgcttat < 1:4576253‑M1/68‑26 (MQ=255) actcgtaGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgcta > 1:800526‑M1/4‑46 (MQ=255) actcgtaGGCCGGATAAGGCGTCCACGCCGCATCCGGCAGTGGTGGcaaatgcctgatgcgctacgcttat < 1:3539727‑M1/68‑26 (MQ=255) actcgtaGGCCGGATAAGGCGTCCACGCCGCATCCGGCAGTGGTGGcaaatgcctgatgcgctacgcttat < 1:3142512‑M1/68‑26 (MQ=255) ctcgtAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgctacgcttat > 1:766880‑M1/3‑45 (MQ=255) ctcgtAGGCCGGATAAGGCGTCCACGCCGCATCCGGCAGTGGTGGcaaatgcctgatgcgctacgcttatc < 1:3266569‑M1/69‑27 (MQ=255) tcgTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatg > 1:3737166‑M1/2‑44 (MQ=255) tcgTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaa > 1:417648‑M1/2‑44 (MQ=255) cGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgctacgctta > 1:3560763‑M1/1‑43 (MQ=255) cGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTGAca < 1:2398275‑M1/45‑3 (MQ=255) gTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgctacgcttatcagg > 1:3620561‑M1/1‑42 (MQ=255) gTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgctacgcttatcag < 1:4664754‑M1/70‑29 (MQ=255) gTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgctacgcttatcag < 1:2527874‑M1/70‑29 (MQ=255) gTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgctacgcttatca < 1:2292632‑M1/69‑28 (MQ=255) gTAGGCCGGATAAGGCGTCCACGCCGCATCCGGCAGTGGTGGcaaatgcctgatgcgctacgcttatca < 1:2061615‑M1/69‑28 (MQ=255) aGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTa > 1:3526884/1‑71 (MQ=255) aGGCCGGATAAGGCGTCCACGCCGCATCCGGCAGTGGTGGcaaatgcctgatgcgctacgcttat < 1:2228200‑M1/65‑26 (MQ=255) ggCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTAt < 1:1179540/71‑1 (MQ=255) ggCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTAt < 1:3121612/71‑1 (MQ=255) gCCGGATAAGGCGTCCACGCCGCATCCGGCAGTGGTGGcaaatgcctgatgcgctacgcttatcaggccta < 1:2665404‑M1/71‑34 (MQ=255) cGGATAAGGCGTCCACGCCGCATCCGGCAGTGGTGGcaaatgcctgatgcgctacgcttatcaggccta > 1:3745869‑M1/1‑36 (MQ=255) ggATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgctacgcttatcaggccta > 1:1051323‑M1/1‑35 (MQ=255) ggATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgctacgcttatca > 1:2540654‑M1/1‑35 (MQ=255) ggATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgctacgcttatca > 1:1768925‑M1/1‑35 (MQ=255) gATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgctacgcttatcaggcctacaga < 1:415688‑M1/71‑38 (MQ=255) gATAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgctacgcttatcaggcctacaga < 1:3744905‑M1/71‑38 (MQ=255) gATAAGGCGTTCACGCCGCATCCGGCAATGGTGAca < 1:860661‑M1/36‑3 (MQ=255) gATAAGGCGTTCACGCCGCATCCGGCAATGGTGAca < 1:2947988‑M1/36‑3 (MQ=255) gATAAGGCGTTAACGCCGCATCCGGCAATGGTGAAc < 1:2174536/36‑1 (MQ=255) aTAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcct < 1:1256639‑M1/42‑10 (MQ=255) aTAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcct < 1:1726267‑M1/42‑10 (MQ=255) aTAAGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcct < 1:3769719‑M1/42‑10 (MQ=255) aTAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGcc < 1:4354912/71‑1 (MQ=255) aTAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGcc < 1:1902689/71‑1 (MQ=255) aTAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGcc < 1:4210460/71‑1 (MQ=255) tAAGGCGTTTACGCCGCATCCGGCAACAGTG‑CCGACTGCCTGATGCGACGCTCGCGCGTCTTATCAGGccg > 1:718344/1‑70 (MQ=1) aaGGCGTTCACGCCGCATCCGGCAATGGTGAcaaatgcctga > 1:60934‑M1/1‑31 (MQ=255) gCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTa < 1:4425751/71‑1 (MQ=255) cGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTaa > 1:5151097/1‑71 (MQ=255) gTTCACGCCGCATCCGGCAATGGTGAcaaatgcctgatgcgctacgcttatcaggcctacaga < 1:651551‑M1/63‑38 (MQ=255) gTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCt < 1:4731550/35‑1 (MQ=255) ttAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTAt < 1:2366829/57‑1 (MQ=255) ttAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGc < 1:3638156/62‑1 (MQ=255) ttAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATc > 1:3411478/1‑71 (MQ=255) ttAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATc > 1:1261933/1‑71 (MQ=255) tAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGc < 1:841510/61‑1 (MQ=255) aaCGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCa > 1:2613281/1‑57 (MQ=255) aaCGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTg > 1:4503588/1‑71 (MQ=255) aaCGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTg > 1:3506308/1‑71 (MQ=255) aaCGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTg > 1:1180973/1‑71 (MQ=255) aaCGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTg > 1:931845/1‑71 (MQ=255) | CGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGA‑TGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTG > Exported/1639197‑1639335 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |