| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,716,859 | A→G | 31.6% | intergenic (+117/+81) | yphH → / ← glyA | predicted DNA‑binding transcriptional regulator/serine hydroxymethyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,716,859 | 0 | A | G | 31.6% | 149.0 / 76.8 | 98 | intergenic (+117/+81) | yphH/glyA | predicted DNA‑binding transcriptional regulator/serine hydroxymethyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (35/32); new base G (15/16); total (50/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.70e-01 | |||||||||||
AACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCA > Exported/1716792‑1716925 | aaCGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAg > 1:5357842/1‑71 (MQ=255)aaCGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAg > 1:621064/1‑71 (MQ=255) caaaTTGCCTGATGCGCTACGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTg < 1:1070969/70‑1 (MQ=11) caaaTTGCCTGATGCGCTACGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTg < 1:1355129/70‑1 (MQ=11) caaaTTGCCTCATGCGCTACGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTg < 1:4129553/70‑1 (MQ=11) agcttgcctgatgcgcgatGCTTATCAGGCCTACCAGAAGATTGCAATATATTGAATTTGCACTGTTTTg < 1:345139‑M2/51‑1 (MQ=255) agcttgcctgatgcgcgatGCTTATCAGGCCTACCAGAAGATTGCAATATATTGAATTTGCACTGTTTTg < 1:334856‑M2/51‑1 (MQ=255) aaaTTGCCTGATGCGCTACGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTg > 1:1029988/1‑70 (MQ=11) aaaTTGCCTGATGCGCTACGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGt < 1:1405716/71‑1 (MQ=11) gcttgcctgatgcgcgatGCTTATCAGGCCTACCAGAAGATTGCAATATATTGAATTTGCACTGTTTTg > 1:100149‑M2/19‑69 (MQ=255) aaTTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGt < 1:1965428/70‑1 (MQ=255) aaTTGCCTGATGCGCTACGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGt < 1:1123517/70‑1 (MQ=11) aTTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAg < 1:280107/71‑1 (MQ=255) ttGCCTGATGCGCTACGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAg > 1:4964856/1‑70 (MQ=11) ccTGATGCGCTACGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAggac < 1:3626879/70‑3 (MQ=11) ccTGATGCGCTACGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAgga < 1:5333843/69‑2 (MQ=11) tGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGa > 1:87508/1‑71 (MQ=255) atgcgcgatGCTTATCAGGCCTACCAGAAGATTGCAATATATTGAATTTGCACTGTTTTGTAGGCCGGATa < 1:276209‑M2/62‑1 (MQ=255) atgcgcgatGCTTATCAGGCCTACCAGAAGATTGCAATATATTGAATTTGCACTGTTTTGTAGGCCGGATa < 1:5482606‑M2/62‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGt > 1:3472443/1‑61 (MQ=255) aTGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGt > 1:3465622/1‑61 (MQ=255) aTGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGt > 1:5241154/1‑61 (MQ=255) aTGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGa < 1:64705/69‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGAt < 1:5506823/70‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGAt < 1:1271959/70‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGAt < 1:1233144/70‑1 (MQ=11) aTGCGCTACGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGAt < 1:4987178/70‑1 (MQ=11) aTGCGCTACGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATa < 1:2054670/71‑1 (MQ=11) tgcgcgatGCTTATCAGGCCTACCAGAAGATTGCAATATATTGAATTTGCACTGTTTTg < 1:5484282‑M2/51‑1 (MQ=255) tgcgcgatGCTTATCAGGCCTACCAGAAGATTGCAATATATTGAATTTGCACTGTTTTg < 1:303370‑M2/51‑1 (MQ=255) cgcTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAgg > 1:1208984/1‑71 (MQ=255) cgcTACGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATAAg < 1:4010410/70‑1 (MQ=11) cgcTACGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATAAg < 1:3703517/70‑1 (MQ=11) cgatGCTTATCAGGCCTACA‑GAAGATTGCAATATATTGAATTTGCACTGTTTTGTAGGCCGGATAAGGCGt < 1:2714889‑M2/67‑1 (MQ=255) atGCTTATCAGGCCTACCAGAAGATTGCAATATATTGAATTTGCACTGtttt < 1:5031911‑M2/50‑1 (MQ=255) atGCTTATCAGGCCTACCAGAAGATAGCAATATATTGAATTTGCACTGTTTTGTAGGCCGGATAAGGCGtt < 1:4203362‑M2/69‑1 (MQ=255) aCGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGa > 1:4984271/1‑62 (MQ=255) aCGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGa > 1:1349458/1‑62 (MQ=255) aCGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAgga > 1:293058/1‑57 (MQ=2) aCGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAgga > 1:5119812/1‑57 (MQ=2) aCGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAgga > 1:5616760/1‑57 (MQ=2) aCGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAgga > 1:4816340/1‑57 (MQ=2) aCGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATAAGGCgct > 1:4413369/1‑69 (MQ=2) aCGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATAAGGCgct > 1:1436366/1‑69 (MQ=2) cGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCaaa > 1:1220582/1‑46 (MQ=255) cGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCaaa > 1:2113532/1‑46 (MQ=255) cGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGtt < 1:5436240/70‑1 (MQ=255) cTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCa > 1:2513460/1‑70 (MQ=255) cTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCa > 1:4071410/1‑70 (MQ=255) cTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCAc > 1:3248722/1‑71 (MQ=255) cTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATATGCGATGTTTTGTAGGACTGATAAGGCGCTcg > 1:5395870/1‑69 (MQ=2) tATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAgga > 1:109984/1‑52 (MQ=2) aTCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATAAGGCGCTcgcg < 1:3798215/69‑1 (MQ=2) tCAGGCCTACCAGAAGATTGCAATATATTGAATTTGCACTGTTTTGTa < 1:4110570/48‑1 (MQ=11) cAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATAAGGCGCTCGcgccg > 1:934475/1‑70 (MQ=2) aGGCCTACCAGAAGATTGCAATATATTGAATTTGCACTGTTTTGTAgg > 1:3956611/1‑48 (MQ=11) aGGCCTACCAGAAGATTGCAATATATTGAATTTGCACTGTTTTGTAgg > 1:3164094/1‑48 (MQ=11) ccTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGAt < 1:5218794/51‑1 (MQ=255) tACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATa < 1:1792834/50‑1 (MQ=255) tACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATa < 1:529082/50‑1 (MQ=255) cacGGAGATTGCAATATATTGAATTTGCAAAGTTTTGt > 1:5162570/1‑38 (MQ=255) cacGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATaa > 1:1550923/1‑49 (MQ=255) cacGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAg > 1:4125468/1‑50 (MQ=255) cacGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAg > 1:1556834/1‑50 (MQ=255) cacGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg < 1:5050166/69‑1 (MQ=255) acGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATaa > 1:5061555/1‑48 (MQ=255) acGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAt > 1:5566762/1‑71 (MQ=255) agaagaTTGCAATATATTGAATTTGCACTGTTTT‑TAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATg > 1:1023432/4‑70 (MQ=11) ggAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGc > 1:3973499/1‑49 (MQ=255) agaTTGCAATATATTGAATTTGCACTGTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGaac < 1:4980081/71‑1 (MQ=11) agaTTGCAATATATTGAATTTGCACTGTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGaac < 1:1028001/71‑1 (MQ=11) agaTTGCAATATATTGAATTTGCACTGTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGaac < 1:1869594/71‑1 (MQ=11) agaTTGCAATATATTGAATTTGCACTGTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGaac < 1:5502856/71‑1 (MQ=11) caTTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATAAGGCGCTCGCGCCGCATCCGGCATCaact < 1:5329792/70‑2 (MQ=11) caTTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATAAGGCGCTCGCGCCGCATCCGGCATCaac > 1:3731757/2‑70 (MQ=11) aTTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATAAGGCGCTCGCGCCGCATCCGGCATcaacaa < 1:3874039/71‑1 (MQ=11) aTTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATa < 1:3329907/41‑1 (MQ=255) tGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGaacaac > 1:1650007/1‑70 (MQ=255) tGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGaacaac > 1:5574960/1‑70 (MQ=255) cAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAg > 1:3828834/1‑71 (MQ=255) aaTATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGc < 1:3284311/71‑1 (MQ=255) atatatTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGca < 1:3494598/71‑1 (MQ=255) atatatTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGca < 1:4537113/71‑1 (MQ=255) atatatTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGca < 1:5076453/71‑1 (MQ=255) tctatTGAATTTGCGATGTTTTGTAGGACGGATAAGGCGCTCGCGCCGCATCCGGCATcaacaa > 1:2837279/3‑64 (MQ=11) tctatTGAATTTGCGATGTTTTGTAGGACGGATAAGGCGCTCGCGCCGCATCCGGCATcaacaa > 1:882821/3‑64 (MQ=11) tatTGAATTTGCGATGTTTTGTAGGACGGATAAGGCGCTCGCGCCGCATCCGGCATCaac > 1:1107182/1‑60 (MQ=11) tatTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGc > 1:2146612/1‑36 (MQ=40) tatTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGcaca < 1:4737930/70‑1 (MQ=255) tatTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACAt < 1:575186/71‑1 (MQ=255) ctttGGATTTGCAAA‑TTTTGTAGACCGGATAAGGAATTCACGCCGCATCCgg > 1:2539797/3‑52 (MQ=0) ctttGGATTTGCAAA‑TTTTGTAGACCGGATAAGGAATTCACGCCGCATCCgg > 1:1595834/3‑52 (MQ=0) ctttGGATTTGCAAA‑TTTTGTAGACCGGATAAGGAATTCACGCCGCATCCgg > 1:1909925/3‑52 (MQ=0) ctttGGATTTGCAAA‑TTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGc < 1:2514547/51‑1 (MQ=0) ttGAATTTGCGATGTTTTGTAGGACGGATAAGGCg < 1:4648181/35‑1 (MQ=14) ttGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCg > 1:2302650/1‑35 (MQ=40) ttGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCg > 1:1378075/1‑35 (MQ=40) gAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGa > 1:3443282/1‑70 (MQ=255) gaTTTGCAAA‑TTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCaa > 1:3873219/2‑53 (MQ=11) tGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCa > 1:5128176/1‑70 (MQ=255) | AACGAAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCA > Exported/1716792‑1716925 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |