| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,716,872 | C→A | 23.2% | intergenic (+130/+68) | yphH → / ← glyA | predicted DNA‑binding transcriptional regulator/serine hydroxymethyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,716,872 | 0 | C | A | 23.2% | 163.1 / 30.9 | 73 | intergenic (+130/+68) | yphH/glyA | predicted DNA‑binding transcriptional regulator/serine hydroxymethyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (28/28); new base A (8/9); total (36/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCT > Exported/1716806‑1716940 | tGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGa > 1:87508/1‑71 (MQ=255) atgcgcgatGCTTATCAGGCCTACCAGAAGATTGCAATATATTGAATTTGCACTGTTTTGTAGGCCGGATa < 1:276209‑M2/62‑1 (MQ=255) atgcgcgatGCTTATCAGGCCTACCAGAAGATTGCAATATATTGAATTTGCACTGTTTTGTAGGCCGGATa < 1:5482606‑M2/62‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGa < 1:64705/69‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGAt < 1:5506823/70‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGAt < 1:1271959/70‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGAt < 1:4987178/70‑1 (MQ=11) aTGCGCTACGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGAt < 1:1233144/70‑1 (MQ=11) aTGCGCTACGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATa < 1:2054670/71‑1 (MQ=11) cgcTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAgg > 1:1208984/1‑71 (MQ=255) cgcTACGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATAAg < 1:3703517/70‑1 (MQ=11) cgcTACGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATAAg < 1:4010410/70‑1 (MQ=11) cgatGCTTATCAGGCCTACA‑GAAGATTGCAATATATTGAATTTGCACTGTTTTGTAGGCCGGATAAGGCGt < 1:2714889‑M2/67‑1 (MQ=255) atGCTTATCAGGCCTACCAGAAGATAGCAATATATTGAATTTGCACTGTTTTGTAGGCCGGATAAGGCGtt < 1:4203362‑M2/69‑1 (MQ=255) aCGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGa > 1:4984271/1‑62 (MQ=255) aCGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGa > 1:1349458/1‑62 (MQ=255) aCGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATAAGGCgct > 1:4413369/1‑69 (MQ=2) aCGCTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATAAGGCgct > 1:1436366/1‑69 (MQ=2) cGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGtt < 1:5436240/70‑1 (MQ=255) cTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCa > 1:4071410/1‑70 (MQ=255) cTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCa > 1:2513460/1‑70 (MQ=255) cTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCAc > 1:3248722/1‑71 (MQ=255) cTTATCAGGCCTACAAGTGCATTGCAATCTATTGAATATGCGATGTTTTGTAGGACTGATAAGGCGCTcg > 1:5395870/1‑69 (MQ=2) aTCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATAAGGCGCTcgcg < 1:3798215/69‑1 (MQ=2) cAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATAAGGCGCTCGcgccg > 1:934475/1‑70 (MQ=2) ccTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGAt < 1:5218794/51‑1 (MQ=255) tACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATa < 1:529082/50‑1 (MQ=255) tACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATa < 1:1792834/50‑1 (MQ=255) cacGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATaa > 1:1550923/1‑49 (MQ=255) cacGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAg > 1:1556834/1‑50 (MQ=255) cacGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAg > 1:4125468/1‑50 (MQ=255) cacGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg < 1:5050166/69‑1 (MQ=255) acGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATaa > 1:5061555/1‑48 (MQ=255) acGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAt > 1:5566762/1‑71 (MQ=255) agaagaTTGCAATATATTGAATTTGCACTGTTTT‑TAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATg > 1:1023432/4‑70 (MQ=11) ggAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGc > 1:3973499/1‑49 (MQ=255) agaTTGCAATATATTGAATTTGCACTGTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGaac < 1:1869594/71‑1 (MQ=11) agaTTGCAATATATTGAATTTGCACTGTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGaac < 1:4980081/71‑1 (MQ=11) agaTTGCAATATATTGAATTTGCACTGTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGaac < 1:5502856/71‑1 (MQ=11) agaTTGCAATATATTGAATTTGCACTGTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGaac < 1:1028001/71‑1 (MQ=11) caTTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATAAGGCGCTCGCGCCGCATCCGGCATCaact < 1:5329792/70‑2 (MQ=11) caTTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATAAGGCGCTCGCGCCGCATCCGGCATCaac > 1:3731757/2‑70 (MQ=11) aTTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATAAGGCGCTCGCGCCGCATCCGGCATcaacaa < 1:3874039/71‑1 (MQ=11) aTTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATa < 1:3329907/41‑1 (MQ=255) tGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGaacaac > 1:5574960/1‑70 (MQ=255) tGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGaacaac > 1:1650007/1‑70 (MQ=255) cAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAg > 1:3828834/1‑71 (MQ=255) aaTATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGc < 1:3284311/71‑1 (MQ=255) atatatTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGca < 1:5076453/71‑1 (MQ=255) atatatTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGca < 1:4537113/71‑1 (MQ=255) atatatTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGca < 1:3494598/71‑1 (MQ=255) tctatTGAATTTGCGATGTTTTGTAGGACGGATAAGGCGCTCGCGCCGCATCCGGCATcaacaa > 1:2837279/3‑64 (MQ=11) tctatTGAATTTGCGATGTTTTGTAGGACGGATAAGGCGCTCGCGCCGCATCCGGCATcaacaa > 1:882821/3‑64 (MQ=11) tatTGAATTTGCGATGTTTTGTAGGACGGATAAGGCGCTCGCGCCGCATCCGGCATCaac > 1:1107182/1‑60 (MQ=11) tatTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGc > 1:2146612/1‑36 (MQ=40) tatTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGcaca < 1:4737930/70‑1 (MQ=255) tatTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACAt < 1:575186/71‑1 (MQ=255) ctttGGATTTGCAAA‑TTTTGTAGACCGGATAAGGAATTCACGCCGCATCCgg > 1:1595834/3‑52 (MQ=0) ctttGGATTTGCAAA‑TTTTGTAGACCGGATAAGGAATTCACGCCGCATCCgg > 1:2539797/3‑52 (MQ=0) ctttGGATTTGCAAA‑TTTTGTAGACCGGATAAGGAATTCACGCCGCATCCgg > 1:1909925/3‑52 (MQ=0) ctttGGATTTGCAAA‑TTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGc < 1:2514547/51‑1 (MQ=0) ttGAATTTGCGATGTTTTGTAGGACGGATAAGGCg < 1:4648181/35‑1 (MQ=14) ttGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCg > 1:1378075/1‑35 (MQ=40) ttGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCg > 1:2302650/1‑35 (MQ=40) gAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGa > 1:3443282/1‑70 (MQ=255) gaTTTGCAAA‑TTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCaa > 1:3873219/2‑53 (MQ=11) tGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCa > 1:5128176/1‑70 (MQ=255) aGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCAc > 1:1399563/1‑71 (MQ=255) aGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCAc > 1:1091837/1‑71 (MQ=255) ttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGt < 1:1625196/70‑1 (MQ=255) ttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGt < 1:1857053/70‑1 (MQ=255) ttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGt < 1:4691661/70‑1 (MQ=255) ggCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCt < 1:810276/71‑1 (MQ=255) | TGATGCGCTACGCTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCT > Exported/1716806‑1716940 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |