| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,716,887 | A→G | 11.4% | intergenic (+145/+53) | yphH → / ← glyA | predicted DNA‑binding transcriptional regulator/serine hydroxymethyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,716,887 | 0 | A | G | 11.4% | 140.5 / 9.2 | 61 | intergenic (+145/+53) | yphH/glyA | predicted DNA‑binding transcriptional regulator/serine hydroxymethyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (31/23); new base G (5/2); total (36/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01 | |||||||||||
CTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGT > Exported/1716818‑1716956 | cTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCa > 1:4071410/1‑70 (MQ=255)cTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCa > 1:2513460/1‑70 (MQ=255)cTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCAc > 1:3248722/1‑71 (MQ=255) aTCAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATAAGGCGCTcgcg < 1:3798215/69‑1 (MQ=2) cAGGCCTACAAGTGCATTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATAAGGCGCTCGcgccg > 1:934475/1‑70 (MQ=2) cacGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg < 1:5050166/69‑1 (MQ=255) acGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAt > 1:5566762/1‑71 (MQ=255) agaagaTTGCAATATATTGAATTTGCACTGTTTT‑TAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATg > 1:1023432/4‑70 (MQ=11) agaTTGCAATATATTGAATTTGCACTGTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGaac < 1:1028001/71‑1 (MQ=11) agaTTGCAATATATTGAATTTGCACTGTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGaac < 1:5502856/71‑1 (MQ=11) agaTTGCAATATATTGAATTTGCACTGTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGaac < 1:4980081/71‑1 (MQ=11) agaTTGCAATATATTGAATTTGCACTGTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGaac < 1:1869594/71‑1 (MQ=11) caTTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATAAGGCGCTCGCGCCGCATCCGGCATCaact < 1:5329792/70‑2 (MQ=11) caTTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATAAGGCGCTCGCGCCGCATCCGGCATCaac > 1:3731757/2‑70 (MQ=11) aTTGCAATCTATTGAATTTGCGATGTTTTGTAGGACGGATAAGGCGCTCGCGCCGCATCCGGCATcaacaa < 1:3874039/71‑1 (MQ=11) tGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGaacaac > 1:1650007/1‑70 (MQ=255) tGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGaacaac > 1:5574960/1‑70 (MQ=255) cAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAg > 1:3828834/1‑71 (MQ=255) aaTATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGc < 1:3284311/71‑1 (MQ=255) atatatTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGca < 1:5076453/71‑1 (MQ=255) atatatTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGca < 1:4537113/71‑1 (MQ=255) atatatTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGca < 1:3494598/71‑1 (MQ=255) tctatTGAATTTGCGATGTTTTGTAGGACGGATAAGGCGCTCGCGCCGCATCCGGCATcaacaa > 1:2837279/3‑64 (MQ=11) tctatTGAATTTGCGATGTTTTGTAGGACGGATAAGGCGCTCGCGCCGCATCCGGCATcaacaa > 1:882821/3‑64 (MQ=11) tatTGAATTTGCGATGTTTTGTAGGACGGATAAGGCGCTCGCGCCGCATCCGGCATCaac > 1:1107182/1‑60 (MQ=11) tatTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGcaca < 1:4737930/70‑1 (MQ=255) tatTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACAt < 1:575186/71‑1 (MQ=255) ctttGGATTTGCAAA‑TTTTGTAGACCGGATAAGGAATTCACGCCGCATCCgg > 1:1909925/3‑52 (MQ=0) ctttGGATTTGCAAA‑TTTTGTAGACCGGATAAGGAATTCACGCCGCATCCgg > 1:1595834/3‑52 (MQ=0) ctttGGATTTGCAAA‑TTTTGTAGACCGGATAAGGAATTCACGCCGCATCCgg > 1:2539797/3‑52 (MQ=0) ctttGGATTTGCAAA‑TTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGc < 1:2514547/51‑1 (MQ=0) gAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGa > 1:3443282/1‑70 (MQ=255) gaTTTGCAAA‑TTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCaa > 1:3873219/2‑53 (MQ=11) tGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCa > 1:5128176/1‑70 (MQ=255) aGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCAc > 1:1091837/1‑71 (MQ=255) aGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCAc > 1:1399563/1‑71 (MQ=255) ttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGt < 1:1625196/70‑1 (MQ=255) ttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGt < 1:1857053/70‑1 (MQ=255) ttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGt < 1:4691661/70‑1 (MQ=255) ggCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCt < 1:810276/71‑1 (MQ=255) gATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGc > 1:1768273/1‑71 (MQ=255) aTAAGGCGTTCACGCCGCATCCTGCATGAACAACGAGCACATTGACAGCAAATCAc > 1:3559667/1‑56 (MQ=255) aTAAGGCGTTCACGCCGCATCCGGCTTGAACAACGAGCACATTGACAGCAAATCAc > 1:2560486/1‑56 (MQ=255) aTAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCAc > 1:1876317/1‑56 (MQ=255) aTAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCAc > 1:3092956/1‑56 (MQ=255) aTAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCAc > 1:2227212/1‑56 (MQ=255) tAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCtt > 1:3746483/1‑65 (MQ=255) aGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGt < 1:5170061/56‑1 (MQ=255) aGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGt < 1:99794/56‑1 (MQ=255) aGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGt < 1:175512/56‑1 (MQ=255) aGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGt < 1:3070448/56‑1 (MQ=255) aGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTa > 1:4653903/1‑70 (MQ=255) gCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTa > 1:5010198/1‑62 (MQ=255) gCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTa > 1:5472104/1‑62 (MQ=255) gCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAAc < 1:3648637/71‑1 (MQ=255) gCGGTCACGCCGCATCCGGCATGAACAACGAGCACATTg < 1:3785733/39‑1 (MQ=255) ttCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAAcc < 1:3534560/69‑1 (MQ=255) tCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCtgg > 1:3691332/1‑71 (MQ=255) tCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCggg > 1:5244874/1‑71 (MQ=255) tCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCggg > 1:1663131/1‑71 (MQ=255) tCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCggg > 1:1768276/1‑71 (MQ=255) cACGCCGCATCCGGCATGAACAACGAGCACATTGa > 1:4074572/1‑35 (MQ=255) cACGCCGCATCCGGCATGAACAACGAGCACATTGa > 1:673666/1‑35 (MQ=255) cACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCggg > 1:643506/1‑70 (MQ=255) aCGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGt < 1:5035597/70‑1 (MQ=255) | CTTATCAGGCCTACACGGAGATTGCAATATATTGAATTTGCAAAGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAACGAGCACATTGACAGCAAATCACCGTTTCGCTTATGCGTAAACCGGGT > Exported/1716818‑1716956 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |