| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,746,079 | C→A | 11.5% | G166G (GGC→GGA) | srmB → | ATP‑dependent RNA helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,746,079 | 0 | C | A | 11.5% | 210.4 / 9.9 | 69 | G166G (GGC→GGA) | srmB | ATP‑dependent RNA helicase |
| Reads supporting (aligned to +/- strand): ref base C (30/31); new base A (3/5); total (33/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
ACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGC > Exported/1746015‑1746147 | aCTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGc < 1:1265895/70‑1 (MQ=255) tCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAg > 1:3588607/1‑71 (MQ=255) cGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCa < 1:4779973/69‑1 (MQ=255) tGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGatat > 1:2686935/1‑71 (MQ=255) tGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGatat > 1:1042860/1‑71 (MQ=255) gCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATc > 1:674286/1‑71 (MQ=255) gcgcgGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCtt < 1:5432963/55‑1 (MQ=255) gcgcgGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCtt < 1:2949589/55‑1 (MQ=255) cgcgGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGa > 1:113931/1‑69 (MQ=255) gcgGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACa < 1:3492741/71‑1 (MQ=255) cgGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACa > 1:4811583/1‑70 (MQ=255) ttGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAAc < 1:5374915/66‑1 (MQ=255) ttGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAAc < 1:4353710/66‑1 (MQ=255) tGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATAtt > 1:2074983/1‑70 (MQ=255) aaaCGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCt < 1:1608963/71‑1 (MQ=255) gCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTgg > 1:5661286/1‑69 (MQ=255) gCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTgg > 1:1894554/1‑69 (MQ=255) gCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTgg > 1:2237582/1‑69 (MQ=255) gATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGaaa > 1:4574408/1‑71 (MQ=255) aTCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGaa < 1:2200044/69‑1 (MQ=255) tCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAAcg > 1:13016/1‑71 (MQ=255) tCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAAc > 1:4128484/1‑70 (MQ=255) ccTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACa < 1:4507421/54‑1 (MQ=255) gacgaAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCt < 1:1464438/70‑1 (MQ=255) atgaggCCGACCGCATGCTGGATATGGGATTtagcgatgccattgatgat < 1:3035133‑M2/48‑20 (MQ=255) acgaAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTgg > 1:3941/1‑71 (MQ=255) aGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTa < 1:2352681/71‑1 (MQ=255) aGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTa < 1:820739/71‑1 (MQ=255) gCCGACCGCATGCTGGATATGGGATTtagcgatgccattgatgatg > 1:5502308‑M2/1‑26 (MQ=255) gCCGACCGCATGCTGGATATGGGATTtagcgatgccattgatg > 1:604080‑M2/1‑26 (MQ=255) gCCGACCGCATGCTGGATATGGGATTtagcgatgccattgatg > 1:953336‑M2/1‑26 (MQ=255) gCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCt > 1:2356918/1‑51 (MQ=255) cAGACCGTATGCTGGATATGGGCGTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCt < 1:4293497/63‑1 (MQ=255) gACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAAc > 1:2222886/1‑56 (MQ=255) gACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAAc > 1:397465/1‑56 (MQ=255) aCCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTaaa > 1:5340464/1‑68 (MQ=255) aCCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTaaa > 1:513643/1‑68 (MQ=255) aCCGCATGCTGGATATGGGATTtagcgatgccattgatgat < 1:1296963‑M2/41‑20 (MQ=255) aCCGCATGCTGGATATGGGATTtagcgatgccattgatgat < 1:1430596‑M2/41‑20 (MQ=255) aCCGCATGCTGGATATGGGATTtagcgatgccattgatga < 1:4334062‑M2/40‑19 (MQ=255) ccGCATGCTGGATATGGGATTtagcgatgccattgatga < 1:2077166‑M2/39‑19 (MQ=255) cGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGAc < 1:338415/71‑1 (MQ=255) cGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTCGCGAAACGCGCTGGCGTaaa < 1:5158084/66‑1 (MQ=255) tATGCTGGATATGGTCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGAccc > 1:1292817/1‑71 (MQ=255) tATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGAccc > 1:2181596/1‑71 (MQ=255) tGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTgg > 1:5094537/1‑43 (MQ=255) tGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTaaa < 1:1765459/62‑1 (MQ=255) tGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTa < 1:829784/60‑1 (MQ=255) tGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAg > 1:1493264/1‑65 (MQ=255) tGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTg < 1:3271073/71‑1 (MQ=255) tGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTg > 1:2495703/1‑71 (MQ=255) tGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTg < 1:224483/71‑1 (MQ=255) tGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTg < 1:2347464/71‑1 (MQ=255) tGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTg < 1:5646632/71‑1 (MQ=255) gCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACgcgc < 1:1273878/52‑1 (MQ=255) cTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGa > 1:95597/1‑44 (MQ=255) cTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGa > 1:4886729/1‑44 (MQ=255) cTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTaaa > 1:1606350/1‑60 (MQ=255) cTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTg > 1:3303003/1‑69 (MQ=255) cTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTg > 1:3228107/1‑69 (MQ=255) gATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCtt < 1:267169/71‑1 (MQ=255) gATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCtt < 1:888634/71‑1 (MQ=255) tatGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCtttt > 1:2138441/1‑71 (MQ=255) ggCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGc < 1:2696519/71‑1 (MQ=255) gCTTCGCTCAGGATATCTAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGc < 1:4076971/70‑1 (MQ=255) gCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAg < 1:4566419/53‑1 (MQ=255) gCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGAc < 1:5438494/55‑1 (MQ=255) gCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGAc < 1:342319/55‑1 (MQ=255) gCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGAc < 1:2021068/55‑1 (MQ=255) | ACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGACCGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGGCGTAAACAGACCCTGCTCTTTTCGGC > Exported/1746015‑1746147 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |