| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,747,030 | C→T | 20.5% | intergenic (+114/+95) | srmB → / ← yfiE | ATP‑dependent RNA helicase/predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,747,030 | 0 | C | T | 20.5% | 162.7 / 30.8 | 73 | intergenic (+114/+95) | srmB/yfiE | ATP‑dependent RNA helicase/predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (25/33); new base T (7/8); total (32/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.38e-01 | |||||||||||
CGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAAT > Exported/1746960‑1747099 | cGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATc < 1:947765/71‑1 (MQ=255)cGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATc < 1:5576898/71‑1 (MQ=255) gCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCt < 1:3997055/71‑1 (MQ=255) aaaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTg < 1:5417556/71‑1 (MQ=255) aaatTGCCTGATGCGCTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGt < 1:3822201‑M2/67‑1 (MQ=255) aaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGt < 1:1747399/71‑1 (MQ=255) aaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGt < 1:5319875/71‑1 (MQ=255) aaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATAAATTGTATTTGCACGATCTTGt < 1:2638081/71‑1 (MQ=255) aaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTg > 1:1255450/1‑69 (MQ=255) ccTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCg < 1:3962906/71‑1 (MQ=255) ccTGATGCGCTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCg < 1:337807/71‑1 (MQ=14) cTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCg > 1:3841755/1‑70 (MQ=255) tGATGCGCTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTg < 1:5583573/62‑1 (MQ=14) gATGCGCTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGa > 1:2652614/1‑70 (MQ=14) aTGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGAt < 1:4543787/70‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGAt < 1:4336819/70‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGAt < 1:2012413/70‑1 (MQ=14) tGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATa < 1:5656641/70‑1 (MQ=255) tGCGCTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATaa < 1:3724856/71‑1 (MQ=14) cgcTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAg < 1:537265/70‑1 (MQ=255) cgcTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTg < 1:2101676/57‑1 (MQ=14) cgcTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTg < 1:1601060/57‑1 (MQ=14) gcTACGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATAAGGc < 1:1839796/71‑1 (MQ=14) cTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCg < 1:3888979/71‑1 (MQ=255) cTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCg < 1:211605/71‑1 (MQ=255) aCGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATaa > 1:4356994/1‑65 (MQ=14) cGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGc > 1:2093406/1‑57 (MQ=14) cGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGc > 1:3622613/1‑57 (MQ=14) cGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATAAGGCGtt > 1:3952435/1‑70 (MQ=14) cGCTTATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATAAGGCGtt > 1:3799248/1‑70 (MQ=14) cTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCAc < 1:116695/71‑1 (MQ=255) ttATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGtt < 1:1500793/67‑1 (MQ=255) tATCAGGCCTACATGGCCCATGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATAAGGCGTTTAcgc > 1:2397133/1‑71 (MQ=11) cAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCAcgccg > 1:2219218/1‑70 (MQ=255) aGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCa < 1:3075374/71‑1 (MQ=255) ccTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAgg < 1:877077/55‑1 (MQ=255) ccTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAgg < 1:5315591/55‑1 (MQ=255) cTACGTGGCTCATGCAATATATTGAATTTGCACGATc > 1:2937576/1‑37 (MQ=255) cGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGc > 1:3182663/1‑52 (MQ=255) gTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATaa > 1:4465721/1‑48 (MQ=255) gTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATaa > 1:4243018/1‑48 (MQ=255) gTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCAt < 1:3191174/64‑1 (MQ=255) gTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCAt < 1:4993722/64‑1 (MQ=255) tCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAAt > 1:4811377/1‑70 (MQ=255) tCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAAt > 1:2646128/1‑70 (MQ=255) aTGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATa > 1:952675/1‑69 (MQ=255) tGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCAcg < 1:2940977/50‑1 (MQ=255) gCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCAcg < 1:2895432/49‑1 (MQ=255) cAATATATTGAATTTGCACGATCTTGTAGGCCGGATaa > 1:5574907/1‑38 (MQ=255) aaTATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCAcg < 1:1655501/47‑1 (MQ=255) tatatTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGaa < 1:2996414/61‑1 (MQ=255) atatTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGa > 1:3934492/1‑59 (MQ=255) tatTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGtt < 1:4305556/39‑1 (MQ=40) tatTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAgcg > 1:3753945/1‑66 (MQ=255) tatTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAg > 1:2210189/1‑64 (MQ=255) tatTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACtt > 1:3855468/1‑71 (MQ=255) tatTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACtt > 1:2994516/1‑71 (MQ=255) ttGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTc > 1:1032134/1‑38 (MQ=40) ttGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCa > 1:5161856/1‑39 (MQ=40) ttGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCa > 1:703815/1‑39 (MQ=40) ttGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCa > 1:5242872/1‑39 (MQ=40) ttGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACttt < 1:1318810/70‑1 (MQ=255) tGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCAcgc > 1:5058831/1‑41 (MQ=38) tGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCAcgc > 1:1350355/1‑41 (MQ=38) aaTTTGCACGATCTTGTAGGCCGGATAAGGCGTTCa > 1:4226580/1‑36 (MQ=40) aaTTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTc < 1:909282/70‑1 (MQ=255) tttGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGAc < 1:3447179/71‑1 (MQ=255) ttGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGAc > 1:1737715/1‑70 (MQ=255) tGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACa < 1:2079870/70‑1 (MQ=255) aCGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGtt < 1:5129410/71‑1 (MQ=255) cGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACtt > 1:3331635/1‑58 (MQ=255) cGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTa < 1:3360371/71‑1 (MQ=255) cGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTa < 1:695727/71‑1 (MQ=255) gATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTa < 1:909579/70‑1 (MQ=255) tCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTa < 1:3397571/68‑1 (MQ=255) tCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAAt > 1:3960700/1‑71 (MQ=255) | CGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGCTCATGCAATATATTGAATTTGCACGATCTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTTTGTCGACAAGTTAAAT > Exported/1746960‑1747099 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |