Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	1,789,035	T→C	71.1%	S58P (TCC→CCC)	ygaF →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	1,789,035	0	T	C	71.1%	62.1 / 27.2	45	S58P (TCC→CCC)	ygaF	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (6/7);  new base C (15/17);  total (21/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAA  >  Exported/1788966‑1789102                                                                      |                                                                    gagTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTc                                                                    <  1:1597900/71‑1 (MQ=255)    tCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCgg                                                                 >  1:2692608/1‑71 (MQ=255)      cGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCgg                                                                 >  1:1960049/1‑69 (MQ=255)       gCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAg                                                              <  1:5433210/71‑1 (MQ=255)          ccGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCt                                                           <  1:435786/71‑1 (MQ=255)          ccGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCt                                                           <  1:2899267/71‑1 (MQ=255)          ccGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCt                                                           <  1:2322233/71‑1 (MQ=255)           cGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTg                                                          <  1:3803805/71‑1 (MQ=255)            ggCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGa                                                         <  1:5209008/71‑1 (MQ=255)             gCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTg                                                          >  1:2963058/1‑69 (MQ=255)                  tCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAg                                                              <  1:4885055/60‑1 (MQ=255)                   cACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTg                                                          >  1:4094842/1‑63 (MQ=255)                   cACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTg                                                          >  1:1382689/1‑63 (MQ=255)                    aCCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAg                                                 <  1:4324420/71‑1 (MQ=255)                      cAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGtt                                               <  1:5689404/71‑1 (MQ=255)                      cAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTg                                                          <  1:3524561/60‑1 (MQ=255)                      cAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGtt                                               <  1:3547602/71‑1 (MQ=255)                       aGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGttt                                              <  1:3828585/71‑1 (MQ=255)                         aCGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTg                                            >  1:1636797/1‑71 (MQ=255)                          cGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGaa                                                        >  1:4792359/1‑58 (MQ=255)                          cGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGaa                                                        >  1:1978897/1‑58 (MQ=255)                             gCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTg                                        >  1:1139189/1‑71 (MQ=255)                                acaacaGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCg                                     >  1:4554514/1‑71 (MQ=255)                                 caacaGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCgg                                    <  1:5414406/71‑1 (MQ=255)                                   acaGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGcc                                                            >  1:2655251/1‑45 (MQ=255)                                   acaGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCggg                                   >  1:2637574/1‑70 (MQ=255)                                    caGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGaa                                 <  1:1466809/71‑1 (MQ=255)                                    caGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGaa                                 <  1:3856484/71‑1 (MQ=255)                                          cgTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTg                                                          <  1:1973810/40‑1 (MQ=255)                                               tCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGccac                       >  1:1071266/1‑70 (MQ=255)                                                ccATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGccacc                      >  1:3914817/1‑70 (MQ=255)                                                ccATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGccacc                      >  1:3346477/1‑70 (MQ=255)                                                 cATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCaa                    <  1:2646321/71‑1 (MQ=255)                                                        gggTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCttt             <  1:1261548/71‑1 (MQ=255)                                                          gTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTg           >  1:4046548/1‑71 (MQ=255)                                                             tATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGa        >  1:4003565/1‑71 (MQ=255)                                                               ttACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGcc                >  1:4701777/1‑61 (MQ=255)                                                               ttACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCtt              <  1:3560112/63‑1 (MQ=255)                                                                tACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATc      >  1:3358569/1‑70 (MQ=255)                                                                 acacGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCa     >  1:562286/1‑70 (MQ=255)                                                                  cacGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCaa    <  1:2671872/70‑1 (MQ=255)                                                                  cacGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCaa    <  1:2489886/70‑1 (MQ=255)                                                                  cacGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCaa    <  1:1836370/70‑1 (MQ=255)                                                                  cacGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCaa    <  1:897470/70‑1 (MQ=255)                                                                   acGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCaaaa  >  1:1450205/1‑71 (MQ=255)                                                                      |                                                                    GAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGTCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAA  >  Exported/1788966‑1789102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑