| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,790,931 | C→A | 8.4% | N259K (AAC→AAA) | gabD → | succinate‑semialdehyde dehydrogenase I, NADP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,790,931 | 0 | C | A | 8.4% | 186.0 / 3.4 | 59 | N259K (AAC→AAA) | gabD | succinate‑semialdehyde dehydrogenase I, NADP‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (28/26); new base A (2/3); total (30/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
AAATTGGCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATT > Exported/1790861‑1790999 | aaaTTGGCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAAc < 1:3805694/71‑1 (MQ=255) ggCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGcc > 1:1020817/1‑71 (MQ=255) ggCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGcc > 1:1129395/1‑71 (MQ=255) ggCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGcc > 1:535697/1‑71 (MQ=255) gccgccAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACgcgcag < 1:3959860/71‑3 (MQ=255) gccgccAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGcc < 1:3916302/70‑1 (MQ=255) gccgccAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGcc < 1:3306941/70‑1 (MQ=255) gccAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGtt > 1:4179409/1‑70 (MQ=255) gccAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGtt > 1:306722/1‑70 (MQ=255) ccAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTa > 1:4385803/1‑71 (MQ=255) ttAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGt < 1:1010572/64‑1 (MQ=255) aaTGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGt > 1:4697145/1‑62 (MQ=255) aaTGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGt > 1:2294923/1‑62 (MQ=255) aaTGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCt > 1:3460380/1‑71 (MQ=255) aTGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCg < 1:4261234/60‑1 (MQ=255) aTGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCg < 1:3118648/60‑1 (MQ=255) aTGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCg < 1:2753019/60‑1 (MQ=255) aTGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGACCTGGGCGGTAACGCGCCg < 1:5350857/60‑1 (MQ=255) ggAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCg > 1:3167787/1‑58 (MQ=255) ggAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGtt > 1:924634/1‑60 (MQ=255) ggAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGtt > 1:1057846/1‑60 (MQ=255) gAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGa > 1:3262891/1‑71 (MQ=255) gAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGa > 1:3594024/1‑71 (MQ=255) aCAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGa < 1:3821496/69‑1 (MQ=255) aCAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACg < 1:1512211/71‑1 (MQ=255) tGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGc < 1:3320921/71‑1 (MQ=255) tGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGc < 1:3327581/71‑1 (MQ=255) cggcgAAAAACATCACCAAAGTGTGTCTGGAATTGGGGGGTAAAGCACCAGCTATCGTAATTGACGATGcc > 1:358294/3‑71 (MQ=255) cgcgAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGcc > 1:3318511/1‑70 (MQ=255) cgcgAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCg < 1:229707/71‑1 (MQ=255) cgcgAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCg < 1:1844167/71‑1 (MQ=255) gcgAAAGACATCAAGAATGTGTCGCTGGAGCTGGGCGGTAACGCGCCg < 1:1402171/48‑1 (MQ=255) aaaaACATCACCAAAGTGTGTCTGGAATTGGGGGGTAAAGCACCAGCTATCGTAATGGACGATGCCGatc > 1:4523038/1‑68 (MQ=255) aaaaACATCACCAAAGTGTGTCTGGAATTGGGGGGTAAAGCACCAGCTATCGTAATGGACGATGCCGa < 1:5694601/68‑1 (MQ=255) aGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGa > 1:3733683/1‑60 (MQ=255) aGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGa > 1:3740376/1‑60 (MQ=255) cATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGAc < 1:65041/70‑1 (MQ=255) tCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATg > 1:2660258/1‑57 (MQ=255) cAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTc > 1:3943463/1‑46 (MQ=255) cAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGa > 1:1855420/1‑54 (MQ=255) cAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGa > 1:5101960/1‑54 (MQ=255) aaGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGAc < 1:1764110/60‑1 (MQ=255) gAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCg > 1:1911374/1‑71 (MQ=255) aaaGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGt > 1:86721/1‑71 (MQ=255) aaaGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGt < 1:2485705/71‑1 (MQ=255) aGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTgg < 1:2463317/71‑1 (MQ=255) gCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGgcg < 1:3464272/71‑1 (MQ=255) atGGAACTGGGCGGTAAATCACCGCTGATCGTTTTCGATGATGCGGATCTCg < 1:4872575/51‑1 (MQ=255) tGGAACTGGGCGGTAAATCACCGCTGATCGTTTTCGATGATGCGGAtctc < 1:1412338/50‑1 (MQ=255) gAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCt > 1:2574603/1‑71 (MQ=255) gAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCt < 1:3867037/71‑1 (MQ=255) cGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCg > 1:1222230/1‑70 (MQ=255) cGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGa < 1:60543/71‑1 (MQ=255) cGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGa < 1:3943606/71‑1 (MQ=255) ggTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGaa < 1:5534347/71‑1 (MQ=255) aCGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTg < 1:1990434/47‑1 (MQ=255) aCGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAg > 1:352267/1‑51 (MQ=255) aCGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAg > 1:577037/1‑51 (MQ=255) aCGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAg > 1:4139746/1‑51 (MQ=255) aCGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAAtt < 1:2484708/70‑1 (MQ=255) | AAATTGGCCGCCAGTTAATGGAACAGTGCGCGAAAGACATCAAGAAAGTGTCGCTGGAGCTGGGCGGTAACGCGCCGTTTATCGTCTTTGACGATGCCGACCTCGACAAAGCCGTGGAAGGCGCGCTGGCCTCGAAATT > Exported/1790861‑1790999 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |