| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 309,867 | T→A | 23.2% | A1002A (GCA→GCT) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 309,867 | 0 | T | A | 23.2% | 137.1 / 40.1 | 69 | A1002A (GCA→GCT) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base T (30/23); new base A (5/11); total (35/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.28e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGC > Exported/309800‑309936 | acaCCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCg > 1:4712527/3‑71 (MQ=255)acaCCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCg > 1:3298615/3‑71 (MQ=255) aCCGGTACGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt < 1:5275557/71‑1 (MQ=255) ggAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTg < 1:2365160/71‑1 (MQ=255) ggAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTg > 1:309971/1‑71 (MQ=255) gtaCGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgc < 1:2736173/69‑1 (MQ=255) gtaCGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgc < 1:2910880/69‑1 (MQ=255) gAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgc < 1:2729606/71‑1 (MQ=255) aCGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgc > 1:2623255/1‑71 (MQ=255) cGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgcg > 1:2352911/1‑71 (MQ=255) cGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt > 1:487488/1‑64 (MQ=255) cGAAGAAGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt > 1:1336105/1‑64 (MQ=255) gaagaaGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTgcgcgc < 1:4858816/71‑1 (MQ=255) gaagaaGATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgcgcgc < 1:1760126/71‑1 (MQ=12) aagaagATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt < 1:1588986/62‑1 (MQ=255) aagaagATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt < 1:4603109/62‑1 (MQ=255) aagaagATTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGtt < 1:2606199/62‑1 (MQ=255) gaagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCgg < 1:1517037/71‑1 (MQ=255) gaagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCgg < 1:1306413/71‑1 (MQ=255) aagaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCATTCTTCGCGCCGGa < 1:448472/71‑1 (MQ=255) gaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAAc > 1:3420077/1‑71 (MQ=255) gaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAAc > 1:3216696/1‑71 (MQ=255) gaTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAAc < 1:91297/71‑1 (MQ=255) aTTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAAc < 1:2801117/70‑1 (MQ=255) aTTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgcgcg < 1:4069408/63‑1 (MQ=255) aTTGCCAGCAACGTTGCAGAGACCATTCCTCCCATTACCCCGATACCCACAGCGTTCTgcgcg < 1:4018313/63‑1 (MQ=255) gCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAg > 1:4999943/1‑70 (MQ=255) tACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAg > 1:1582566/1‑71 (MQ=255) aCCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAg > 1:5516986/1‑70 (MQ=255) ccGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTa > 1:2665121/1‑71 (MQ=255) gTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACt > 1:1012766/1‑71 (MQ=255) ttGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGGGTTCTGCGCGCCGGAACCAGCACCAGTACt > 1:4175240/1‑70 (MQ=255) cGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGAt < 1:468344/70‑1 (MQ=255) gTCACCATCCCGCCCATTACACCGGTACCTACTGCGt < 1:4352798/37‑1 (MQ=255) gTCACCATCCCGCCCATTACACCGGTACCTACTGCGt < 1:4097617/37‑1 (MQ=255) gTCACCATCCCGCCCATTACACCGGTACCTACTGCGt < 1:5169313/37‑1 (MQ=255) gTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATaa < 1:2850347/70‑1 (MQ=255) tCACCATCCCGCCCATTACACCGGTACCTACTGCg < 1:719907/35‑1 (MQ=255) aCCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAg < 1:2622107/71‑1 (MQ=255) cATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAAc > 1:1679130/1‑66 (MQ=255) tCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGa < 1:1986799/43‑1 (MQ=255) tCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTg > 1:2983748/1‑59 (MQ=255) tCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCgg > 1:2489622/1‑70 (MQ=255) ccGCCCATTACACCGGTACCTACTGCGTTCTgcgc > 1:4285740/1‑35 (MQ=255) ccGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCa > 1:2221300/1‑70 (MQ=255) cGCCCATTACACCGGTACCTACTGCGTTCTGCGGGCCGGAACCAGCAcc < 1:1454465/49‑1 (MQ=255) gCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAg > 1:4182180/1‑44 (MQ=255) gCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAg > 1:1062951/1‑44 (MQ=255) ccATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACg > 1:3367986/1‑71 (MQ=255) aTTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGcc < 1:5062096/71‑1 (MQ=255) aTTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGcc < 1:4369787/71‑1 (MQ=255) ttACCCCGATACCCACAGCGTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGcc > 1:2923481/1‑70 (MQ=255) tACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGcc > 1:2110669/1‑69 (MQ=255) tACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGcc > 1:2464934/1‑69 (MQ=255) acacCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAg > 1:2989727/1‑71 (MQ=255) ccccGATACCCACAGCGTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAg < 1:4620256/68‑1 (MQ=255) ccccGATACCCACAGCGTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAg < 1:3655006/68‑1 (MQ=255) cacCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATaa > 1:3283800/1‑63 (MQ=255) cGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATa < 1:5235571/71‑1 (MQ=255) cGGTACCTACTGCGTTCTCCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGAt > 1:1016418/1‑70 (MQ=255) ggTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATaa < 1:4032596/71‑1 (MQ=255) gTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATaaa < 1:5169148/71‑1 (MQ=255) aCCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACg > 1:4015345/1‑71 (MQ=255) ccTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGc < 1:4728093/48‑1 (MQ=255) ccTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATaa > 1:3501355/1‑55 (MQ=255) tACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGc > 1:1976242/1‑69 (MQ=255) aCTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATaa > 1:515694/1‑52 (MQ=255) aCTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAg > 1:1142514/1‑71 (MQ=255) cTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGc > 1:4513458/1‑71 (MQ=255) | ATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGC > Exported/309800‑309936 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |