| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,826,285 | A→G | 32.2% | I13I (ATT→ATC) | hydN ← | formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,826,285 | 0 | A | G | 32.2% | 171.4 / 117.7 | 143 | I13I (ATT→ATC) | hydN | formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit |
| Reads supporting (aligned to +/- strand): ref base A (60/37); new base G (25/21); total (85/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 146 total aligned reads displayed.
GGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCC > Exported/1826216‑1826354 | ggTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCaa < 1:2852499/71‑1 (MQ=255) aGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACTCACGCTACTACACAGGTACGGCAACCAATACa > 1:508002/1‑71 (MQ=255) aGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATAc < 1:3326430/70‑1 (MQ=255) aGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACa > 1:1007127/1‑71 (MQ=255) cGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACAtt < 1:1007139/71‑1 (MQ=255) cGATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACAtt < 1:4151053/71‑1 (MQ=255) aTGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTa < 1:1973093/71‑1 (MQ=255) caatCCTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTcgc < 1:4541114/68‑1 (MQ=255) caatCCTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTcgc < 1:3192130/68‑1 (MQ=255) caatCCTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTcgc < 1:1102946/68‑1 (MQ=255) aatCCTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTcgca > 1:649410/3‑70 (MQ=255) gTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACt > 1:133643/1‑66 (MQ=255) gTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACt > 1:4059290/1‑66 (MQ=255) atCCTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTcgcat > 1:4393609/2‑69 (MQ=255) atCCTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTcgcat > 1:1623890/2‑69 (MQ=255) atCCTGAATCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTcgcat < 1:1165970/70‑3 (MQ=255) cTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAg > 1:4335482/1‑71 (MQ=255) cTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAg > 1:880577/1‑71 (MQ=255) cTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACAt > 1:1394768/1‑58 (MQ=255) cTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACAt > 1:1124460/1‑58 (MQ=255) cTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACAt > 1:4947987/1‑58 (MQ=255) cTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATccg > 1:1523518/1‑69 (MQ=255) gATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACt > 1:3799546/1‑61 (MQ=255) gATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACt > 1:3681554/1‑61 (MQ=255) gATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGc > 1:4850430/1‑70 (MQ=255) aTTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCaa > 1:5036326/1‑71 (MQ=255) aTTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCa < 1:2285167/70‑1 (MQ=255) aTTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACAt > 1:2524375/1‑55 (MQ=255) aTTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGc < 1:3267245/69‑1 (MQ=255) aTTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCa < 1:3064599/70‑1 (MQ=255) tCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAatgat > 1:4631497/1‑71 (MQ=255) atgatgcGACACTGCGCAAGCCACTTCACAGGTACGGCACCCGATACATTTCGTCGCATCCGCaataata > 1:4067795/2‑69 (MQ=255) atgatgcGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCaataata < 1:5204141/69‑2 (MQ=255) atgatgcGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAataa < 1:2683741/67‑3 (MQ=255) gatgcGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATaaac > 1:3092677/1‑70 (MQ=255) atgcGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACAttt > 1:1321704/1‑48 (MQ=19) atgaGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAAc > 1:860411/1‑71 (MQ=255) atgaGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAAc > 1:5350741/1‑71 (MQ=255) atgaGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGaaa < 1:2500988/70‑1 (MQ=255) atgaGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAAc > 1:4810690/1‑71 (MQ=255) atgaGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAAc > 1:2564220/1‑71 (MQ=255) tgaGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACg > 1:1061345/1‑71 (MQ=255) gagaAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACgg > 1:4285982/1‑71 (MQ=255) agaAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGt > 1:4197328/1‑71 (MQ=255) gaAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGt > 1:3715697/1‑70 (MQ=255) aaaCCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTtc < 1:1646897/71‑1 (MQ=255) aaaCCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAg < 1:5528802/54‑1 (MQ=255) actgcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATAAACCGGTtcat > 1:1878049/4‑71 (MQ=255) aCCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCa > 1:1485272/1‑54 (MQ=255) aCCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCa > 1:3727298/1‑54 (MQ=255) ctgcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATAAACCGGTtcatg < 1:437939/69‑2 (MQ=255) tgcgcAAGCCACTTCACAGGTACGGCAACCGATACAtt < 1:3410947/37‑1 (MQ=25) tgcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAat < 1:863635/53‑1 (MQ=255) gcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCaataata > 1:685906/1‑56 (MQ=255) gcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCaataat > 1:4456837/1‑56 (MQ=255) gcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATAAACCGGTtcatgat > 1:3055161/1‑68 (MQ=255) gcgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATAAACCGGTtcatgat > 1:3596661/1‑68 (MQ=255) acgcACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTtcatca > 1:788160/2‑70 (MQ=255) acgcACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTtcatca > 1:4714803/2‑70 (MQ=255) acgcACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTtcatca > 1:2827556/2‑70 (MQ=255) cgcACGCTACTTCACAGGTACGGCAACCAATACATTTACTcgc < 1:975174/43‑1 (MQ=255) cgcACGCTACTTCACAGGTACGGCAACCAATACATTTACTcgc < 1:1759731/43‑1 (MQ=255) cgcACGCTACTTCACAGGTACGGCAACCAATACATTTACTcgc < 1:3769048/43‑1 (MQ=255) cgcACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGt > 1:2429850/1‑45 (MQ=255) cgcACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGt > 1:2350963/1‑45 (MQ=255) cgcACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGt > 1:4551204/1‑45 (MQ=255) cgcACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGaaa > 1:2286834/1‑59 (MQ=255) cgcAAGCCACTTCACAGGTACGGCAACCGATACAtttc > 1:653348/1‑37 (MQ=25) cgcAAGCCACTTCACAGGTACGGCAACCGATACAtttc > 1:2159770/1‑37 (MQ=25) cgcAAGCCACTTCACAGGTACGGCAACCGATACAtttc > 1:4546304/1‑37 (MQ=25) cgcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCAATAATaaacc < 1:1726768/60‑3 (MQ=255) gcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCaataataa < 1:3399526/56‑3 (MQ=255) gcAAGCCACTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCaataataa < 1:2872217/56‑3 (MQ=255) cACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAAtt < 1:4459305/71‑1 (MQ=255) aCGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATtc < 1:1406533/71‑1 (MQ=255) cGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAAtgatga < 1:220667/53‑1 (MQ=255) cTTCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCaataata < 1:1849232/47‑2 (MQ=255) cTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGaaa > 1:2833369/1‑50 (MQ=255) cTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGc < 1:4611331/71‑1 (MQ=255) ttCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAAc < 1:780530/50‑1 (MQ=255) tCACAGGTACGGCAACCGATACATTTCGTCGCATCCGCaa > 1:1186528/1‑40 (MQ=255) cacaGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACgg > 1:2277003/1‑50 (MQ=255) cacaGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACTGTTCATCAAATTCTCCAGCAAt > 1:2634725/1‑71 (MQ=255) cacaGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCaa > 1:761459/1‑70 (MQ=255) cacaGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAAt > 1:4893344/1‑71 (MQ=255) cacaGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAAt > 1:3200815/1‑71 (MQ=255) acaGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCGAGCAAt < 1:4551119/70‑1 (MQ=255) acaGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAAt < 1:1570595/70‑1 (MQ=255) ggTACGGCAACCAATACATTTACTCGCGTCAGCAatgat > 1:2382824/1‑39 (MQ=255) ggTACGGCAACCAATACATTTACTCGCGTCAGCAatgat > 1:2881310/1‑39 (MQ=255) ggTACGGCAACCAATACATTTACTCGCGTCAGCAatgat > 1:3002386/1‑39 (MQ=255) tACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGt < 1:3427/71‑1 (MQ=255) aCGGCAACCAATACATTTACTCGCGTCAGCAAttatga < 1:4235802/38‑1 (MQ=255) aCGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGt < 1:3380428/70‑1 (MQ=255) cGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGtt < 1:3680/70‑1 (MQ=255) ggCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGAc < 1:266824/65‑1 (MQ=255) gCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTaa > 1:3059631/1‑70 (MQ=255) aCCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATgcg > 1:5187152/1‑71 (MQ=255) cAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCa > 1:5682124/1‑54 (MQ=255) cAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGcc > 1:3609962/1‑71 (MQ=255) | GGTCAGCGATGCACAGTCCTGATTTTCCTGATGAGAAACCGCGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCC > Exported/1826216‑1826354 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |