Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,837,426 T→C 5.9% T331T (ACA→ACG nlpD ← predicted outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,837,4260TC 5.9% 246.5 / 4.1 85T331T (ACA→ACGnlpDpredicted outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (38/42);  new base C (2/3);  total (40/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

GTTCCGGTGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGC  >  Exported/1837358‑1837496
                                                                    |                                                                      
gTTCCGGTGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCAtt                                                                        <  1:244915/69‑1 (MQ=255)
     ggTGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGGATTGTGTCGt                                                                 >  1:3249609/1‑71 (MQ=255)
     ggTGCTACCCATGGTCGCTATTTTTTGCCCAGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGt                                                                 <  1:2943337/71‑1 (MQ=255)
      gTGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGtt                                                                <  1:897355/71‑1 (MQ=255)
        gCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATtg                                                                       >  1:3591377/1‑62 (MQ=255)
          tACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa                                                               <  1:648261/68‑1 (MQ=255)
             ccATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa                                                               >  1:5222695/1‑65 (MQ=255)
             ccATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCg                                                         <  1:5219593/71‑1 (MQ=255)
                  gTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAgg                                                     <  1:3042154/70‑1 (MQ=255)
                   tCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATgg                                                            <  1:909798/62‑1 (MQ=255)
                      cTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa                                                               <  1:2625171/56‑1 (MQ=255)
                      cTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa                                                               <  1:2993430/56‑1 (MQ=255)
                        aTTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAg                                              >  1:593596/1‑71 (MQ=255)
                         ttttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTc                                                                   >  1:2695819/1‑49 (MQ=255)
                         ttttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGt                                                        <  1:3075263/60‑1 (MQ=255)
                         ttttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCa                                                   >  1:951753/1‑65 (MQ=255)
                         ttttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCa                                                   >  1:2703336/1‑65 (MQ=255)
                         ttttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAgg                                             >  1:2161948/1‑71 (MQ=255)
                         ttttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAgg                                             >  1:935329/1‑71 (MQ=255)
                          tttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa                                                               >  1:4263030/1‑52 (MQ=255)
                           ttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa                                                               >  1:5317070/1‑51 (MQ=255)
                           ttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa                                                               >  1:4668441/1‑51 (MQ=255)
                           ttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa                                                               >  1:5074047/1‑51 (MQ=255)
                           ttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa                                                               >  1:4807759/1‑51 (MQ=255)
                           ttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGt                                            >  1:3680270/1‑70 (MQ=255)
                           ttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGt                                            >  1:725762/1‑70 (MQ=255)
                            tttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGt                                            >  1:3164911/1‑69 (MQ=255)
                            tttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTa                                           <  1:3755482/70‑1 (MQ=255)
                            tttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTa                                           <  1:1873702/70‑1 (MQ=255)
                             ttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTaa                                          <  1:3033293/70‑1 (MQ=255)
                                 cccGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa                                                               >  1:2421201/1‑45 (MQ=255)
                                 cccGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa                                                               >  1:883277/1‑45 (MQ=255)
                                  ccGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAatcat                                      <  1:2586968/69‑1 (MQ=255)
                                  ccGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAatcat                                      <  1:2746298/69‑1 (MQ=255)
                                   cGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAAtcatca                                    >  1:2363276/1‑70 (MQ=255)
                                   cGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATcatcat                                   >  1:3523343/1‑71 (MQ=255)
                                   cGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATcatcat                                   >  1:577200/1‑71 (MQ=255)
                                   cGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATcatcat                                   >  1:1830435/1‑71 (MQ=255)
                                    gCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCga                                                                 >  1:5524239/1‑39 (MQ=255)
                                    gCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATcatcat                                   >  1:1943963/1‑70 (MQ=255)
                                    gCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATcatcat                                   >  1:4166284/1‑70 (MQ=255)
                                     ccTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTa                                 >  1:1506986/1‑71 (MQ=255)
                                      cTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTAt                                >  1:3004534/1‑71 (MQ=255)
                                       ttAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAatcat                                      <  1:3249820/64‑1 (MQ=255)
                                       ttAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAatcat                                      <  1:992869/64‑1 (MQ=255)
                                         aaCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTa                                           >  1:5319443/1‑57 (MQ=255)
                                         aaCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTa                                           >  1:2614351/1‑57 (MQ=255)
                                         aaCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGt                              >  1:3185901/1‑70 (MQ=255)
                                          aCTTCTTGTTGTTCCCGGACCAGGATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGttt                            <  1:2500831/71‑1 (MQ=255)
                                          aCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGttt                            <  1:3627885/71‑1 (MQ=255)
                                          aCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGttt                            <  1:5127570/71‑1 (MQ=255)
                                          aCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGttt                            <  1:5594504/71‑1 (MQ=255)
                                          aCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGttt                            <  1:2222326/71‑1 (MQ=255)
                                          aCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt                             <  1:877407/70‑1 (MQ=255)
                                           cttcttGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt                             <  1:2011674/69‑1 (MQ=255)
                                           cttcttGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt                             <  1:3885381/69‑1 (MQ=255)
                                           cttcttGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt                             <  1:254132/69‑1 (MQ=255)
                                               ttgttgTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTa                                           <  1:4508513/51‑1 (MQ=255)
                                                tgttgtTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgatga                       >  1:3877715/1‑70 (MQ=255)
                                                 gttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgatga                       <  1:5424680/69‑1 (MQ=255)
                                                 gttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgatga                       <  1:4754187/69‑1 (MQ=255)
                                                 gttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTgatgat                      <  1:4205122/70‑1 (MQ=255)
                                                  ttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATa                     <  1:343340/70‑1 (MQ=255)
                                                    gttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgat                         >  1:3393886/1‑64 (MQ=255)
                                                     ttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATc                  <  1:1129091/70‑1 (MQ=255)
                                                     ttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATc                  <  1:470958/70‑1 (MQ=255)
                                                     ttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCa                 <  1:4905618/71‑1 (MQ=255)
                                                       cccGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCa                                               >  1:1274032/1‑39 (MQ=255)
                                                       cccGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt                             >  1:2303730/1‑57 (MQ=255)
                                                         tttaCCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGAt              <  1:216349/67‑1 (MQ=255)
                                                         tttaCCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGAt              <  1:940574/67‑1 (MQ=255)
                                                         cGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGAtt             <  1:2104168/71‑1 (MQ=255)
                                                              cAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTAt                                <  1:918072/47‑1 (MQ=255)
                                                              cAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGt        <  1:210467/71‑1 (MQ=255)
                                                               aGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGt        <  1:2273518/70‑1 (MQ=255)
                                                                gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGt                              >  1:4418962/1‑47 (MQ=255)
                                                                gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGt                              >  1:1609507/1‑47 (MQ=255)
                                                                gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGt                              >  1:3298963/1‑47 (MQ=255)
                                                                gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGt                              >  1:4194240/1‑47 (MQ=255)
                                                                gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTgatgat                      <  1:760127/55‑1 (MQ=255)
                                                                gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTgatgat                      <  1:3767932/55‑1 (MQ=255)
                                                                gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTgatgat                      <  1:5178212/55‑1 (MQ=255)
                                                                gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg      <  1:4288213/71‑1 (MQ=255)
                                                                gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg      <  1:969777/71‑1 (MQ=255)
                                                                gCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAg      >  1:4278880/1‑71 (MQ=255)
                                                                 cATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGc     <  1:169398/71‑1 (MQ=255)
                                                                 cATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGc     >  1:5110646/1‑71 (MQ=255)
                                                                   ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTa                                 <  1:398584/41‑1 (MQ=255)
                                                                   ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTa                                 <  1:4052296/41‑1 (MQ=255)
                                                                   ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgat                         >  1:1587023/1‑49 (MQ=255)
                                                                   ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCg         >  1:2007803/1‑65 (MQ=255)
                                                                   ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg      >  1:1663468/1‑68 (MQ=255)
                                                                   ttGTGTAGTTATGGGCGTAGGCACTCAGGTAATCATCATTa                                 <  1:503388/41‑1 (MQ=255)
                                                                    tgtgTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcgc  >  1:4724543/1‑71 (MQ=255)
                                                                    tgtgTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcg   >  1:4044360/1‑70 (MQ=255)
                                                                    tgtgTCGTTATGGGAGTAGGCACTCATGTAATCATCATTATGTTTGATGATAATCATATTAACg         >  1:5221032/1‑64 (MQ=255)
                                                                    |                                                                      
GTTCCGGTGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGC  >  Exported/1837358‑1837496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: