Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,837,432 G→A 12.4% N329N (AAC→AAT nlpD ← predicted outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,837,4320GA12.4% 271.3 / 23.0 105N329N (AAC→AATnlpDpredicted outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (45/47);  new base A (9/4);  total (54/51)
Fisher's exact test for biased strand distribution p-value = 2.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 110 total aligned reads displayed.

GGTGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCG  >  Exported/1837363‑1837502
                                                                     |                                                                      
ggTGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGGATTGTGTCGt                                                                       >  1:3249609/1‑71 (MQ=255)
ggTGCTACCCATGGTCGCTATTTTTTGCCCAGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGt                                                                       <  1:2943337/71‑1 (MQ=255)
 gTGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGtt                                                                      <  1:897355/71‑1 (MQ=255)
     tACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa                                                                     <  1:648261/68‑1 (MQ=255)
        ccATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa                                                                     >  1:5222695/1‑65 (MQ=255)
        ccATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCg                                                               <  1:5219593/71‑1 (MQ=255)
             gTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAgg                                                           <  1:3042154/70‑1 (MQ=255)
              tCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATgg                                                                  <  1:909798/62‑1 (MQ=255)
                 cTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa                                                                     <  1:2993430/56‑1 (MQ=255)
                 cTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa                                                                     <  1:2625171/56‑1 (MQ=255)
                   aTTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAg                                                    >  1:593596/1‑71 (MQ=255)
                    ttttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGt                                                              <  1:3075263/60‑1 (MQ=255)
                    ttttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCa                                                         >  1:951753/1‑65 (MQ=255)
                    ttttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAgg                                                   >  1:935329/1‑71 (MQ=255)
                    ttttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAgg                                                   >  1:2161948/1‑71 (MQ=255)
                      ttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa                                                                     >  1:4807759/1‑51 (MQ=255)
                      ttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa                                                                     >  1:4668441/1‑51 (MQ=255)
                      ttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa                                                                     >  1:5074047/1‑51 (MQ=255)
                      ttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGt                                                  >  1:725762/1‑70 (MQ=255)
                      ttttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGt                                                  >  1:3680270/1‑70 (MQ=255)
                       tttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGt                                                  >  1:3164911/1‑69 (MQ=255)
                        ttGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTaa                                                <  1:3033293/70‑1 (MQ=255)
                            cccGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa                                                                     >  1:2421201/1‑45 (MQ=255)
                            cccGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTa                                                                     >  1:883277/1‑45 (MQ=255)
                             ccGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAatcat                                            <  1:2586968/69‑1 (MQ=255)
                             ccGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAatcat                                            <  1:2746298/69‑1 (MQ=255)
                              cGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATcatcat                                         >  1:577200/1‑71 (MQ=255)
                              cGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATcatcat                                         >  1:3523343/1‑71 (MQ=255)
                              cGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATcatcat                                         >  1:1830435/1‑71 (MQ=255)
                               gCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCga                                                                       >  1:5524239/1‑39 (MQ=255)
                               gCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATcatcat                                         >  1:4166284/1‑70 (MQ=255)
                               gCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATcatcat                                         >  1:1943963/1‑70 (MQ=255)
                                ccTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTa                                       >  1:1506986/1‑71 (MQ=255)
                                 cTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTAt                                      >  1:3004534/1‑71 (MQ=255)
                                  ttAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAatcat                                            <  1:3249820/64‑1 (MQ=255)
                                    aaCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTa                                                 >  1:2614351/1‑57 (MQ=255)
                                    aaCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTa                                                 >  1:5319443/1‑57 (MQ=255)
                                     aCTTCTTGTTGTTCCCGGACCAGGATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGttt                                  <  1:2500831/71‑1 (MQ=255)
                                     aCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGttt                                  <  1:2222326/71‑1 (MQ=255)
                                     aCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGttt                                  <  1:5594504/71‑1 (MQ=255)
                                     aCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGttt                                  <  1:3627885/71‑1 (MQ=255)
                                     aCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGttt                                  <  1:5127570/71‑1 (MQ=255)
                                     aCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt                                   <  1:877407/70‑1 (MQ=255)
                                      cttcttGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt                                   <  1:2011674/69‑1 (MQ=255)
                                      cttcttGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt                                   <  1:254132/69‑1 (MQ=255)
                                      cttcttGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt                                   <  1:3885381/69‑1 (MQ=255)
                                          ttgttgTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTa                                                 <  1:4508513/51‑1 (MQ=255)
                                           tgttgtTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgatga                             >  1:3877715/1‑70 (MQ=255)
                                            gttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgatga                             <  1:4754187/69‑1 (MQ=255)
                                            gttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgatga                             <  1:5424680/69‑1 (MQ=255)
                                            gttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTgatgat                            <  1:4205122/70‑1 (MQ=255)
                                             ttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATa                           <  1:343340/70‑1 (MQ=255)
                                               gttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgat                               >  1:3393886/1‑64 (MQ=255)
                                                ttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATc                        <  1:470958/70‑1 (MQ=255)
                                                ttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATc                        <  1:1129091/70‑1 (MQ=255)
                                                ttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCa                       <  1:4905618/71‑1 (MQ=255)
                                                  cccGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCa                                                     >  1:1274032/1‑39 (MQ=255)
                                                  cccGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt                                   >  1:2303730/1‑57 (MQ=255)
                                                    tttaCCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGAt                    <  1:216349/67‑1 (MQ=255)
                                                    tttaCCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGAt                    <  1:940574/67‑1 (MQ=255)
                                                    cGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGAtt                   <  1:2104168/71‑1 (MQ=255)
                                                         cAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTAt                                      <  1:918072/47‑1 (MQ=255)
                                                         cAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGt              <  1:210467/71‑1 (MQ=255)
                                                          aGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGt              <  1:2273518/70‑1 (MQ=255)
                                                           gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGt                                    >  1:3298963/1‑47 (MQ=255)
                                                           gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGt                                    >  1:4194240/1‑47 (MQ=255)
                                                           gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGt                                    >  1:4418962/1‑47 (MQ=255)
                                                           gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGt                                    >  1:1609507/1‑47 (MQ=255)
                                                           gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTgatgat                            <  1:5178212/55‑1 (MQ=255)
                                                           gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTgatgat                            <  1:3767932/55‑1 (MQ=255)
                                                           gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg            <  1:969777/71‑1 (MQ=255)
                                                           gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg            <  1:4288213/71‑1 (MQ=255)
                                                           gCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAg            >  1:4278880/1‑71 (MQ=255)
                                                            cATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGc           <  1:169398/71‑1 (MQ=255)
                                                            cATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGc           >  1:5110646/1‑71 (MQ=255)
                                                             atcgtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGc           >  1:2584178/4‑70 (MQ=255)
                                                              ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTa                                       <  1:4052296/41‑1 (MQ=255)
                                                              ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTa                                       <  1:398584/41‑1 (MQ=255)
                                                              ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgat                               >  1:1587023/1‑49 (MQ=255)
                                                              ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCg               >  1:2007803/1‑65 (MQ=255)
                                                              ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg            >  1:1663468/1‑68 (MQ=255)
                                                              ttGTGTAGTTATGGGCGTAGGCACTCAGGTAATCATCATTa                                       <  1:503388/41‑1 (MQ=255)
                                                               tgtgTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcgc        >  1:4724543/1‑71 (MQ=255)
                                                               tgtgTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcg         >  1:4044360/1‑70 (MQ=255)
                                                               tgtgTCGTTATGGGAGTAGGCACTCATGTAATCATCATTATGTTTGATGATAATCATATTAACg               >  1:5221032/1‑64 (MQ=255)
                                                                gtgtCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcgcg       >  1:1290134/1‑71 (MQ=255)
                                                                gtgtCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcgcg       >  1:3937281/1‑71 (MQ=255)
                                                                gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGccac        <  1:3115612/70‑3 (MQ=255)
                                                                gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCcacg       >  1:865233/1‑71 (MQ=255)
                                                                gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCcacg       >  1:2447017/1‑71 (MQ=255)
                                                                gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCcacg       >  1:3550347/1‑71 (MQ=255)
                                                                gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACAGTAGCcacg       >  1:5366931/1‑71 (MQ=255)
                                                                 tgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATgaga                     >  1:744114/1‑56 (MQ=255)
                                                                 tgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATgaga                     >  1:3683978/1‑56 (MQ=255)
                                                                   tCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtga                                >  1:5143050/1‑43 (MQ=255)
                                                                   tCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAg                      <  1:5070809/53‑1 (MQ=255)
                                                                     gTTATGGGCGTAGGCACTCAGGTAATCATCATTAt                                      <  1:2391443/35‑1 (MQ=255)
                                                                     gTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAgcg  >  1:279509/1‑71 (MQ=255)
                                                                     gTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAgcg  >  1:171463/1‑71 (MQ=255)
                                                                     gTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAg  >  1:2485721/1‑69 (MQ=255)
                                                                     |                                                                      
GGTGCTACCCATGGTCGCTATTTTTTGCCCCGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCG  >  Exported/1837363‑1837502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: