Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,837,463 T→C 28.7% N319S (AAT→AGT)  nlpD ← predicted outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,837,4630TC28.7% 138.0 / 70.4 101N319S (AAT→AGT) nlpDpredicted outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (33/39);  new base C (17/12);  total (50/51)
Fisher's exact test for biased strand distribution p-value = 2.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.37e-01

Only 100 of 115 total aligned reads displayed.

CGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCT  >  Exported/1837393‑1837531
                                                                      |                                                                    
cGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATcatcat                                                                      >  1:577200/1‑71 (MQ=255)
cGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATcatcat                                                                      >  1:3523343/1‑71 (MQ=255)
 gCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATcatcat                                                                      >  1:1943963/1‑70 (MQ=255)
  ccTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTa                                                                    >  1:1506986/1‑71 (MQ=255)
   cTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTAt                                                                   >  1:3004534/1‑71 (MQ=255)
      aaCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGt                                                                 >  1:3185901/1‑70 (MQ=255)
       aCTTCTTGTTGTTCCCGGACCAGGATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGttt                                                               <  1:2500831/71‑1 (MQ=255)
       aCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGttt                                                               <  1:5594504/71‑1 (MQ=255)
       aCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGttt                                                               <  1:2222326/71‑1 (MQ=255)
       aCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGttt                                                               <  1:5127570/71‑1 (MQ=255)
       aCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGttt                                                               <  1:3627885/71‑1 (MQ=255)
       aCTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt                                                                <  1:877407/70‑1 (MQ=255)
        cttcttGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt                                                                <  1:254132/69‑1 (MQ=255)
        cttcttGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt                                                                <  1:2011674/69‑1 (MQ=255)
        cttcttGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt                                                                <  1:3885381/69‑1 (MQ=255)
             tgttgtTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgatga                                                          >  1:3877715/1‑70 (MQ=255)
              gttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgatga                                                          <  1:4754187/69‑1 (MQ=255)
              gttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgatga                                                          <  1:5424680/69‑1 (MQ=255)
              gttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTgatgat                                                         <  1:4205122/70‑1 (MQ=255)
               ttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATa                                                        <  1:343340/70‑1 (MQ=255)
                 gttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgat                                                            >  1:3393886/1‑64 (MQ=255)
                  ttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATc                                                     <  1:470958/70‑1 (MQ=255)
                  ttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATc                                                     <  1:1129091/70‑1 (MQ=255)
                  ttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCa                                                    <  1:4905618/71‑1 (MQ=255)
                    cccGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGtt                                                                >  1:2303730/1‑57 (MQ=255)
                      tttaCCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGAt                                                 <  1:940574/67‑1 (MQ=255)
                      tttaCCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGAt                                                 <  1:216349/67‑1 (MQ=255)
                      cGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGAtt                                                <  1:2104168/71‑1 (MQ=255)
                           cAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTAt                                                                   <  1:918072/47‑1 (MQ=255)
                           cAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGt                                           <  1:210467/71‑1 (MQ=255)
                            aGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGt                                           <  1:2273518/70‑1 (MQ=255)
                             gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGt                                                                 >  1:1609507/1‑47 (MQ=255)
                             gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGt                                                                 >  1:4194240/1‑47 (MQ=255)
                             gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGt                                                                 >  1:3298963/1‑47 (MQ=255)
                             gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTgatgat                                                         <  1:760127/55‑1 (MQ=255)
                             gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTgatgat                                                         <  1:5178212/55‑1 (MQ=255)
                             gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTgatgat                                                         <  1:3767932/55‑1 (MQ=255)
                             gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg                                         <  1:4288213/71‑1 (MQ=255)
                             gCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAg                                         >  1:4278880/1‑71 (MQ=255)
                              cATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGc                                        <  1:169398/71‑1 (MQ=255)
                              cATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGc                                        >  1:5110646/1‑71 (MQ=255)
                                ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTa                                                                    <  1:4052296/41‑1 (MQ=255)
                                ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTa                                                                    <  1:398584/41‑1 (MQ=255)
                                ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgat                                                            >  1:1587023/1‑49 (MQ=255)
                                ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCg                                            >  1:2007803/1‑65 (MQ=255)
                                ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg                                         >  1:1663468/1‑68 (MQ=255)
                                ttGTGTAGTTATGGGCGTAGGCACTCAGGTAATCATCATTa                                                                    <  1:503388/41‑1 (MQ=255)
                                 tgtgTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcgc                                     >  1:4724543/1‑71 (MQ=255)
                                 tgtgTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcg                                      >  1:4044360/1‑70 (MQ=255)
                                 tgtgTCGTTATGGGAGTAGGCACTCATGTAATCATCATTATGTTTGATGATAATCATATTAACg                                            >  1:5221032/1‑64 (MQ=255)
                                  gtgtCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcgcg                                    >  1:1290134/1‑71 (MQ=255)
                                  gtgtCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcgcg                                    >  1:3937281/1‑71 (MQ=255)
                                  gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCcacg                                    >  1:2447017/1‑71 (MQ=255)
                                  gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCcacg                                    >  1:865233/1‑71 (MQ=255)
                                  gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACAGTAGCcacg                                    >  1:5366931/1‑71 (MQ=255)
                                   tgtCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGttt                                                               <  1:4988662/43‑1 (MQ=255)
                                   tgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATgaga                                                  >  1:3683978/1‑56 (MQ=255)
                                   tgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATgaga                                                  >  1:744114/1‑56 (MQ=255)
                                     tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAg                                         >  1:832223/4‑63 (MQ=255)
                                     tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCa                                  <  1:4151842/67‑1 (MQ=255)
                                     tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCa                                  <  1:147037/67‑1 (MQ=255)
                                     tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAg                                 >  1:4608661/4‑71 (MQ=255)
                                     tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAg                                 >  1:4552884/4‑71 (MQ=255)
                                     tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAg                                 >  1:435145/4‑71 (MQ=255)
                                     tCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtga                                                             >  1:5143050/1‑43 (MQ=255)
                                     tCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAg                                                   <  1:5070809/53‑1 (MQ=255)
                                      cattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTAcc                                             >  1:1162839/3‑58 (MQ=255)
                                      cattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAg                                 >  1:5488917/3‑70 (MQ=255)
                                       gTTATGGGCGTAGGCACTCAGGTAATCATCATTAt                                                                   <  1:2391443/35‑1 (MQ=255)
                                       gTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAgcg                               >  1:171463/1‑71 (MQ=255)
                                       gTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAgcg                               >  1:279509/1‑71 (MQ=255)
                                       gTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAga                                >  1:2485721/1‑69 (MQ=255)
                                       attATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGAt                                                 >  1:2727593/2‑53 (MQ=255)
                                       attATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTAcc                                             >  1:3314409/2‑57 (MQ=255)
                                       attATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGccac                                     <  1:2958374/64‑3 (MQ=255)
                                       attATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAgct                               <  1:4579251/70‑2 (MQ=255)
                                        ttATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAgctg                              <  1:5610259/71‑3 (MQ=255)
                                        taaTGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCa                                  <  1:1184967/65‑1 (MQ=255)
                                         tATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAgcgc                              >  1:4100491/1‑70 (MQ=255)
                                           tGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGtt                           <  1:2870711/71‑1 (MQ=255)
                                            gggCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATaa                                                       >  1:3229625/1‑42 (MQ=255)
                                            gggCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAAt                                                      >  1:4626672/1‑43 (MQ=255)
                                            gggCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAAt                                                      >  1:4294273/1‑43 (MQ=255)
                                              gCGTAGGCACTCAGGTAATCATCATTATGTTTgatgat                                                         <  1:1852372/38‑1 (MQ=255)
                                                 tAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCa                                                    <  1:1068833/40‑1 (MQ=255)
                                                   ggCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCa                    >  1:2287529/1‑70 (MQ=255)
                                                    gCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATa                  <  1:1831448/71‑1 (MQ=255)
                                                       cTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTAcc                        <  1:4423282/62‑1 (MQ=255)
                                                         cAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaacaa             >  1:4076349/1‑71 (MQ=255)
                                                           ggTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg                                         <  1:1838597/41‑1 (MQ=255)
                                                           ggTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAAcg           >  1:1033989/1‑71 (MQ=255)
                                                            gTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATa                  <  1:893411/63‑1 (MQ=255)
                                                              aaTCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaac               >  1:2230364/1‑64 (MQ=255)
                                                                 catcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAg                                 >  1:3919408/1‑43 (MQ=255)
                                                                 catcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAg                                 >  1:2394416/1‑43 (MQ=255)
                                                                 catcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaac               <  1:1029486/61‑1 (MQ=255)
                                                                  atcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaacaa             >  1:2835029/1‑62 (MQ=255)
                                                                  atcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCAt    <  1:43273/71‑1 (MQ=255)
                                                                     atTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCt  <  1:5115252/70‑1 (MQ=255)
                                                                      ttATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATaaa                >  1:4966377/1‑55 (MQ=255)
                                                                      |                                                                    
CGCCTTAACTTCTTGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCT  >  Exported/1837393‑1837531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: