Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,837,471 G→A 36.7% I316I (ATC→ATT nlpD ← predicted outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,837,4710GA36.7% 69.7 / 70.9 79I316I (ATC→ATTnlpDpredicted outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (28/22);  new base A (17/12);  total (45/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

TGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGC  >  Exported/1837406‑1837539
                                                                 |                                                                    
tgttgtTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgatga                                                                  >  1:3877715/1‑70 (MQ=255)
 gttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgatga                                                                  <  1:5424680/69‑1 (MQ=255)
 gttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgatga                                                                  <  1:4754187/69‑1 (MQ=255)
 gttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTgatgat                                                                 <  1:4205122/70‑1 (MQ=255)
  ttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATa                                                                <  1:343340/70‑1 (MQ=255)
    gttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgat                                                                    >  1:3393886/1‑64 (MQ=255)
     ttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATc                                                             <  1:470958/70‑1 (MQ=255)
     ttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATc                                                             <  1:1129091/70‑1 (MQ=255)
     ttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCa                                                            <  1:4905618/71‑1 (MQ=255)
         tttaCCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGAt                                                         <  1:940574/67‑1 (MQ=255)
         tttaCCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGAt                                                         <  1:216349/67‑1 (MQ=255)
         cGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGAtt                                                        <  1:2104168/71‑1 (MQ=255)
              cAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGt                                                   <  1:210467/71‑1 (MQ=255)
               aGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGt                                                   <  1:2273518/70‑1 (MQ=255)
                gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTgatgat                                                                 <  1:760127/55‑1 (MQ=255)
                gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTgatgat                                                                 <  1:5178212/55‑1 (MQ=255)
                gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTgatgat                                                                 <  1:3767932/55‑1 (MQ=255)
                gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg                                                 <  1:4288213/71‑1 (MQ=255)
                gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg                                                 <  1:969777/71‑1 (MQ=255)
                gCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAg                                                 >  1:4278880/1‑71 (MQ=255)
                 cATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGc                                                <  1:169398/71‑1 (MQ=255)
                 cATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGc                                                >  1:5110646/1‑71 (MQ=255)
                  atcgtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGc                                                >  1:2584178/4‑70 (MQ=255)
                   ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtgat                                                                    >  1:1587023/1‑49 (MQ=255)
                   ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCg                                                    >  1:2007803/1‑65 (MQ=255)
                   ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg                                                 >  1:1663468/1‑68 (MQ=255)
                    tgtgTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcgc                                             >  1:4724543/1‑71 (MQ=255)
                    tgtgTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcg                                              >  1:4044360/1‑70 (MQ=255)
                    tgtgTCGTTATGGGAGTAGGCACTCATGTAATCATCATTATGTTTGATGATAATCATATTAACg                                                    >  1:5221032/1‑64 (MQ=255)
                     gtgtCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcgcg                                            >  1:3937281/1‑71 (MQ=255)
                     gtgtCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcgcg                                            >  1:1290134/1‑71 (MQ=255)
                     gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGccac                                             <  1:3115612/70‑3 (MQ=255)
                     gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCcacg                                            >  1:865233/1‑71 (MQ=255)
                     gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCcacg                                            >  1:3550347/1‑71 (MQ=255)
                     gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCcacg                                            >  1:2447017/1‑71 (MQ=255)
                     gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACAGTAGCcacg                                            >  1:5366931/1‑71 (MQ=255)
                      tgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATgaga                                                          >  1:744114/1‑56 (MQ=255)
                      tgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATgaga                                                          >  1:3683978/1‑56 (MQ=255)
                        tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAg                                                 >  1:832223/4‑63 (MQ=255)
                        tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCa                                          <  1:147037/67‑1 (MQ=255)
                        tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCa                                          <  1:4151842/67‑1 (MQ=255)
                        tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAg                                         >  1:4608661/4‑71 (MQ=255)
                        tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAg                                         >  1:4552884/4‑71 (MQ=255)
                        tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAg                                         >  1:435145/4‑71 (MQ=255)
                        tCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTtga                                                                     >  1:5143050/1‑43 (MQ=255)
                        tCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAg                                                           <  1:5070809/53‑1 (MQ=255)
                         cattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTAcc                                                     >  1:1162839/3‑58 (MQ=255)
                         cattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAg                                         >  1:5488917/3‑70 (MQ=255)
                          gTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAgcg                                       >  1:171463/1‑71 (MQ=255)
                          gTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAgcg                                       >  1:279509/1‑71 (MQ=255)
                          gTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAga                                        >  1:2485721/1‑69 (MQ=255)
                          attATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGAt                                                         >  1:2727593/2‑53 (MQ=255)
                          attATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTAcc                                                     >  1:3314409/2‑57 (MQ=255)
                          attATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGccac                                             <  1:2958374/64‑3 (MQ=255)
                          attATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAgct                                       <  1:4579251/70‑2 (MQ=255)
                           ttATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAgctg                                      <  1:2302439/71‑3 (MQ=255)
                           ttATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAgctg                                      <  1:5610259/71‑3 (MQ=255)
                           ttATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAgctg                                      <  1:4749363/71‑3 (MQ=255)
                           taaTGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCa                                          <  1:1184967/65‑1 (MQ=255)
                            tATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAgcgc                                      >  1:4100491/1‑70 (MQ=255)
                              tGGGCGTAGGCACTCAGGTAATCATCATTTTGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGt                                    >  1:1414191/1‑70 (MQ=255)
                              tGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGtt                                   <  1:2870711/71‑1 (MQ=255)
                               gggCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATaa                                                               >  1:3229625/1‑42 (MQ=255)
                               gggCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAAt                                                              >  1:4294273/1‑43 (MQ=255)
                               gggCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAAt                                                              >  1:4626672/1‑43 (MQ=255)
                                 gCGTAGGCACTCAGGTAATCATCATTATGTTTgatgat                                                                 <  1:1852372/38‑1 (MQ=255)
                                    tAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCa                                                            <  1:1068833/40‑1 (MQ=255)
                                      ggCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCa                            >  1:2287529/1‑70 (MQ=255)
                                       gCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATa                          <  1:1831448/71‑1 (MQ=255)
                                          cTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTAcc                                <  1:4423282/62‑1 (MQ=255)
                                          cTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaac                       <  1:2100593/71‑1 (MQ=255)
                                            cAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaacaa                     >  1:4076349/1‑71 (MQ=255)
                                              ggTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg                                                 <  1:1838597/41‑1 (MQ=255)
                                              ggTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAAcg                   >  1:1033989/1‑71 (MQ=255)
                                               gTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATa                          <  1:893411/63‑1 (MQ=255)
                                                 aaTCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaac                       >  1:2230364/1‑64 (MQ=255)
                                                 aaTCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaac                       >  1:775615/1‑64 (MQ=255)
                                                 aaTCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaac                       >  1:5385861/1‑64 (MQ=255)
                                                    catcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAg                                         >  1:2394416/1‑43 (MQ=255)
                                                    catcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAg                                         >  1:3969487/1‑43 (MQ=255)
                                                    catcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAg                                         >  1:3919408/1‑43 (MQ=255)
                                                    catcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaac                       <  1:1029486/61‑1 (MQ=255)
                                                     atcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaacaa                     >  1:2835029/1‑62 (MQ=255)
                                                     atcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCAt            <  1:43273/71‑1 (MQ=255)
                                                        atTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCt          <  1:5096701/70‑1 (MQ=255)
                                                        atTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCt          <  1:5115252/70‑1 (MQ=255)
                                                         ttATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATaaa                        >  1:4966377/1‑55 (MQ=255)
                                                            tGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaacaa                     >  1:4051478/1‑55 (MQ=255)
                                                             gTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTc    >  1:2657380/1‑71 (MQ=255)
                                                              tttGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTa                                  <  1:4623498/40‑1 (MQ=255)
                                                                tgatgaTAATCAGATTACCGTAGCCGCGCAGCGCGTTAcc                                >  1:3657265/1‑40 (MQ=255)
                                                                tgatgaTAATCAGATTACCGTAGCCGCGCAGCGCGTTAcc                                >  1:2486644/1‑40 (MQ=255)
                                                                tgatgaTAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAg                              >  1:1107523/1‑42 (MQ=255)
                                                                tgatgaTAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTcgc  <  1:4085857/70‑1 (MQ=255)
                                                                 |                                                                    
TGTTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGC  >  Exported/1837406‑1837539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: