Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,837,477 A→G 33.3% I314I (ATT→ATC nlpD ← predicted outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,837,4770AG33.3% 91.9 / 72.0 87I314I (ATT→ATCnlpDpredicted outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (32/26);  new base G (17/12);  total (49/38)
Fisher's exact test for biased strand distribution p-value = 8.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTG  >  Exported/1837408‑1837547
                                                                     |                                                                      
ttgttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATa                                                                        <  1:343340/70‑1 (MQ=255)
   ttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATc                                                                     <  1:1129091/70‑1 (MQ=255)
   ttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATc                                                                     <  1:470958/70‑1 (MQ=255)
   ttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCa                                                                    <  1:4905618/71‑1 (MQ=255)
       tttaCCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGAt                                                                 <  1:940574/67‑1 (MQ=255)
       tttaCCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGAt                                                                 <  1:216349/67‑1 (MQ=255)
       cGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGAtt                                                                <  1:2104168/71‑1 (MQ=255)
            cAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGt                                                           <  1:210467/71‑1 (MQ=255)
             aGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGt                                                           <  1:2273518/70‑1 (MQ=255)
              gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg                                                         <  1:969777/71‑1 (MQ=255)
              gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg                                                         <  1:4288213/71‑1 (MQ=255)
              gCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAg                                                         >  1:4278880/1‑71 (MQ=255)
               cATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGc                                                        <  1:169398/71‑1 (MQ=255)
               cATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGc                                                        >  1:5110646/1‑71 (MQ=255)
                atcgtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGc                                                        >  1:2584178/4‑70 (MQ=255)
                 ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCg                                                            >  1:2007803/1‑65 (MQ=255)
                 ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg                                                         >  1:1663468/1‑68 (MQ=255)
                  tgtgTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcgc                                                     >  1:4724543/1‑71 (MQ=255)
                  tgtgTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcg                                                      >  1:4044360/1‑70 (MQ=255)
                  tgtgTCGTTATGGGAGTAGGCACTCATGTAATCATCATTATGTTTGATGATAATCATATTAACg                                                            >  1:5221032/1‑64 (MQ=255)
                   gtgtCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcgcg                                                    >  1:3937281/1‑71 (MQ=255)
                   gtgtCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcgcg                                                    >  1:1290134/1‑71 (MQ=255)
                   gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGccac                                                     <  1:3115612/70‑3 (MQ=255)
                   gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCcacg                                                    >  1:865233/1‑71 (MQ=255)
                   gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCcacg                                                    >  1:3550347/1‑71 (MQ=255)
                   gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCcacg                                                    >  1:2447017/1‑71 (MQ=255)
                   gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACAGTAGCcacg                                                    >  1:5366931/1‑71 (MQ=255)
                    tgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATgaga                                                                  >  1:744114/1‑56 (MQ=255)
                    tgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATgaga                                                                  >  1:3683978/1‑56 (MQ=255)
                      tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAg                                                         >  1:832223/4‑63 (MQ=255)
                      tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCa                                                  <  1:4151842/67‑1 (MQ=255)
                      tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCa                                                  <  1:147037/67‑1 (MQ=255)
                      tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAg                                                 >  1:435145/4‑71 (MQ=255)
                      tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAg                                                 >  1:4552884/4‑71 (MQ=255)
                      tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAg                                                 >  1:4608661/4‑71 (MQ=255)
                      tCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAg                                                                   <  1:5070809/53‑1 (MQ=255)
                       cattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTAcc                                                             >  1:1162839/3‑58 (MQ=255)
                       cattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAg                                                 >  1:5488917/3‑70 (MQ=255)
                        gTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAgcg                                               >  1:279509/1‑71 (MQ=255)
                        gTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAgcg                                               >  1:171463/1‑71 (MQ=255)
                        gTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAga                                                >  1:2485721/1‑69 (MQ=255)
                        attATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGAt                                                                 >  1:2727593/2‑53 (MQ=255)
                        attATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTAcc                                                             >  1:3314409/2‑57 (MQ=255)
                        attATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGccac                                                     <  1:2958374/64‑3 (MQ=255)
                        attATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAgct                                               <  1:4579251/70‑2 (MQ=255)
                         ttATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAgctg                                              <  1:4749363/71‑3 (MQ=255)
                         ttATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAgctg                                              <  1:2302439/71‑3 (MQ=255)
                         ttATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAgctg                                              <  1:5610259/71‑3 (MQ=255)
                         taaTGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCa                                                  <  1:1184967/65‑1 (MQ=255)
                          tATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAgcgc                                              >  1:4100491/1‑70 (MQ=255)
                            tGGGCGTAGGCACTCAGGTAATCATCATTTTGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGt                                            >  1:1414191/1‑70 (MQ=255)
                            tGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGtt                                           <  1:2870711/71‑1 (MQ=255)
                             gggCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATaa                                                                       >  1:3229625/1‑42 (MQ=255)
                             gggCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAAt                                                                      >  1:4626672/1‑43 (MQ=255)
                             gggCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAAt                                                                      >  1:4294273/1‑43 (MQ=255)
                                  tAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCa                                                                    <  1:1068833/40‑1 (MQ=255)
                                    ggCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCa                                    >  1:2287529/1‑70 (MQ=255)
                                     gCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATa                                  <  1:1831448/71‑1 (MQ=255)
                                        cTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTAcc                                        <  1:4423282/62‑1 (MQ=255)
                                        cTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaac                               <  1:2100593/71‑1 (MQ=255)
                                          cAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaacaa                             >  1:4076349/1‑71 (MQ=255)
                                            ggTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg                                                         <  1:1838597/41‑1 (MQ=255)
                                            ggTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAAcg                           >  1:1033989/1‑71 (MQ=255)
                                             gTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATa                                  <  1:893411/63‑1 (MQ=255)
                                               aaTCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaac                               >  1:775615/1‑64 (MQ=255)
                                               aaTCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaac                               >  1:5385861/1‑64 (MQ=255)
                                               aaTCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaac                               >  1:2230364/1‑64 (MQ=255)
                                                  catcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAg                                                 >  1:2394416/1‑43 (MQ=255)
                                                  catcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAg                                                 >  1:3969487/1‑43 (MQ=255)
                                                  catcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAg                                                 >  1:3919408/1‑43 (MQ=255)
                                                  catcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaac                               <  1:1029486/61‑1 (MQ=255)
                                                   atcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaacaa                             >  1:2835029/1‑62 (MQ=255)
                                                   atcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCAt                    <  1:43273/71‑1 (MQ=255)
                                                      atTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCt                  <  1:5115252/70‑1 (MQ=255)
                                                      atTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCt                  <  1:5096701/70‑1 (MQ=255)
                                                       ttATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATaaa                                >  1:4966377/1‑55 (MQ=255)
                                                          tGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaacaa                             >  1:4051478/1‑55 (MQ=255)
                                                           gTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTc            >  1:2657380/1‑71 (MQ=255)
                                                            tttGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTa                                          <  1:4623498/40‑1 (MQ=255)
                                                              tgatgaTAATCAGATTACCGTAGCCGCGCAGCGCGTTAcc                                        >  1:3657265/1‑40 (MQ=255)
                                                              tgatgaTAATCAGATTACCGTAGCCGCGCAGCGCGTTAcc                                        >  1:2486644/1‑40 (MQ=255)
                                                              tgatgaTAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAg                                      >  1:1107523/1‑42 (MQ=255)
                                                              tgatgaTAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTcgc          <  1:4085857/70‑1 (MQ=255)
                                                                atgatAATCAGATTACCGTAGCCGCGCAGCGCGTTAc                                         >  1:2184482/1‑37 (MQ=255)
                                                                atgatAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCg               <  1:5142663/63‑1 (MQ=255)
                                                                atgatAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGAt       <  1:1269571/71‑1 (MQ=255)
                                                                atgatAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGAt       <  1:4127558/71‑1 (MQ=255)
                                                                 tgatAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGAt       <  1:2406421/70‑1 (MQ=255)
                                                                  gatAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATaa     >  1:2650116/1‑71 (MQ=255)
                                                                     aaTCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGAt       <  1:2561805/66‑1 (MQ=255)
                                                                     aaTCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTg  >  1:274092/1‑71 (MQ=255)
                                                                     |                                                                      
TTGTTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTG  >  Exported/1837408‑1837547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: