Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,837,480 C→G 31.4% L313L (CTG→CTC nlpD ← predicted outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,837,4800CG31.4% 106.7 / 68.2 86L313L (CTG→CTCnlpDpredicted outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (32/27);  new base G (15/12);  total (47/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

TTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTG  >  Exported/1837411‑1837547
                                                                     |                                                                   
ttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATc                                                                     <  1:1129091/70‑1 (MQ=255)
ttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATc                                                                     <  1:470958/70‑1 (MQ=255)
ttCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCa                                                                    <  1:4905618/71‑1 (MQ=255)
    tttaCCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGAt                                                                 <  1:940574/67‑1 (MQ=255)
    tttaCCAGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGAt                                                                 <  1:216349/67‑1 (MQ=255)
    cGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGAtt                                                                <  1:2104168/71‑1 (MQ=255)
         cAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGt                                                           <  1:210467/71‑1 (MQ=255)
          aGCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGt                                                           <  1:2273518/70‑1 (MQ=255)
           gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg                                                         <  1:969777/71‑1 (MQ=255)
           gCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg                                                         <  1:4288213/71‑1 (MQ=255)
           gCATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAg                                                         >  1:4278880/1‑71 (MQ=255)
            cATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGc                                                        <  1:169398/71‑1 (MQ=255)
            cATCGTGTCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGc                                                        >  1:5110646/1‑71 (MQ=255)
             atcgtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGc                                                        >  1:2584178/4‑70 (MQ=255)
              ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCg                                                            >  1:2007803/1‑65 (MQ=255)
              ttGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg                                                         >  1:1663468/1‑68 (MQ=255)
               tgtgTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcgc                                                     >  1:4724543/1‑71 (MQ=255)
               tgtgTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcg                                                      >  1:4044360/1‑70 (MQ=255)
               tgtgTCGTTATGGGAGTAGGCACTCATGTAATCATCATTATGTTTGATGATAATCATATTAACg                                                            >  1:5221032/1‑64 (MQ=255)
                gtgtCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcgcg                                                    >  1:3937281/1‑71 (MQ=255)
                gtgtCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCcgcg                                                    >  1:1290134/1‑71 (MQ=255)
                gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGccac                                                     <  1:3115612/70‑3 (MQ=255)
                gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCcacg                                                    >  1:3550347/1‑71 (MQ=255)
                gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCcacg                                                    >  1:865233/1‑71 (MQ=255)
                gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCcacg                                                    >  1:2447017/1‑71 (MQ=255)
                gtgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACAGTAGCcacg                                                    >  1:5366931/1‑71 (MQ=255)
                 tgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATgaga                                                                  >  1:744114/1‑56 (MQ=255)
                 tgtCATTATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATgaga                                                                  >  1:3683978/1‑56 (MQ=255)
                   tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAg                                                         >  1:832223/4‑63 (MQ=255)
                   tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCa                                                  <  1:4151842/67‑1 (MQ=255)
                   tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCa                                                  <  1:147037/67‑1 (MQ=255)
                   tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAg                                                 >  1:435145/4‑71 (MQ=255)
                   tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAg                                                 >  1:4552884/4‑71 (MQ=255)
                   tcattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAg                                                 >  1:4608661/4‑71 (MQ=255)
                   tCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAg                                                                   <  1:5070809/53‑1 (MQ=255)
                    cattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTAcc                                                             >  1:1162839/3‑58 (MQ=255)
                    cattATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAg                                                 >  1:5488917/3‑70 (MQ=255)
                     gTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAgcg                                               >  1:171463/1‑71 (MQ=255)
                     gTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAgcg                                               >  1:279509/1‑71 (MQ=255)
                     gTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAga                                                >  1:2485721/1‑69 (MQ=255)
                     attATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGAt                                                                 >  1:2727593/2‑53 (MQ=255)
                     attATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTAcc                                                             >  1:3314409/2‑57 (MQ=255)
                     attATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGccac                                                     <  1:2958374/64‑3 (MQ=255)
                     attATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAgct                                               <  1:4579251/70‑2 (MQ=255)
                      ttATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAgctg                                              <  1:2302439/71‑3 (MQ=255)
                      ttATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAgctg                                              <  1:5610259/71‑3 (MQ=255)
                      ttATGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCAgctg                                              <  1:4749363/71‑3 (MQ=255)
                      taaTGGGCGTAAGCCGTAATGTAATCTTCACTGTGTTTAATCATGATGAGATTACCGTAGCCACGCa                                                  <  1:1184967/65‑1 (MQ=255)
                       tATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAgcgc                                              >  1:4100491/1‑70 (MQ=255)
                         tGGGCGTAGGCACTCAGGTAATCATCATTTTGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGt                                            >  1:1414191/1‑70 (MQ=255)
                         tGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGtt                                           <  1:2870711/71‑1 (MQ=255)
                               tAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCa                                                                    <  1:1068833/40‑1 (MQ=255)
                                 ggCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCa                                    >  1:2287529/1‑70 (MQ=255)
                                  gCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATa                                  <  1:1831448/71‑1 (MQ=255)
                                     cTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTAcc                                        <  1:4423282/62‑1 (MQ=255)
                                     cTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaac                               <  1:2100593/71‑1 (MQ=255)
                                       cAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaacaa                             >  1:4076349/1‑71 (MQ=255)
                                         ggTAATCATCATTATGTTTGATGATAATCAGATTACCGTAg                                                         <  1:1838597/41‑1 (MQ=255)
                                         ggTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAAcg                           >  1:1033989/1‑71 (MQ=255)
                                          gTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATa                                  <  1:893411/63‑1 (MQ=255)
                                            aaTCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaac                               >  1:2230364/1‑64 (MQ=255)
                                            aaTCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaac                               >  1:5385861/1‑64 (MQ=255)
                                            aaTCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaac                               >  1:775615/1‑64 (MQ=255)
                                               catcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAg                                                 >  1:2394416/1‑43 (MQ=255)
                                               catcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAg                                                 >  1:3919408/1‑43 (MQ=255)
                                               catcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAg                                                 >  1:3969487/1‑43 (MQ=255)
                                               catcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaac                               <  1:1029486/61‑1 (MQ=255)
                                                atcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaacaa                             >  1:2835029/1‑62 (MQ=255)
                                                atcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCAt                    <  1:43273/71‑1 (MQ=255)
                                                   atTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCt                  <  1:5115252/70‑1 (MQ=255)
                                                   atTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCt                  <  1:5096701/70‑1 (MQ=255)
                                                    ttATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATaaa                                >  1:4966377/1‑55 (MQ=255)
                                                       tGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAaacaa                             >  1:4051478/1‑55 (MQ=255)
                                                        gTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTc            >  1:2657380/1‑71 (MQ=255)
                                                         tttGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTa                                          <  1:4623498/40‑1 (MQ=255)
                                                           tgatgaTAATCAGATTACCGTAGCCGCGCAGCGCGTTAcc                                        >  1:2486644/1‑40 (MQ=255)
                                                           tgatgaTAATCAGATTACCGTAGCCGCGCAGCGCGTTAcc                                        >  1:3657265/1‑40 (MQ=255)
                                                           tgatgaTAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAg                                      >  1:1107523/1‑42 (MQ=255)
                                                           tgatgaTAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTcgc          <  1:4085857/70‑1 (MQ=255)
                                                             atgatAATCAGATTACCGTAGCCGCGCAGCGCGTTAc                                         >  1:2184482/1‑37 (MQ=255)
                                                             atgatAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCg               <  1:5142663/63‑1 (MQ=255)
                                                             atgatAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGAt       <  1:4127558/71‑1 (MQ=255)
                                                             atgatAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGAt       <  1:1269571/71‑1 (MQ=255)
                                                              tgatAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGAt       <  1:2406421/70‑1 (MQ=255)
                                                               gatAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATaa     >  1:2650116/1‑71 (MQ=255)
                                                                  aaTCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGAt       <  1:2561805/66‑1 (MQ=255)
                                                                  aaTCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTg  >  1:274092/1‑71 (MQ=255)
                                                                   aTCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTTCGGTCGCGATa      <  1:1361126/66‑1 (MQ=255)
                                                                   aTCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATa      <  1:4202495/66‑1 (MQ=255)
                                                                    tCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTg  >  1:1283768/1‑69 (MQ=255)
                                                                     cAGATTACCGTAGCCGCGCAGCGCGTTACCAGCAt                                   >  1:2079547/1‑35 (MQ=255)
                                                                     |                                                                   
TTCCCGGACCAGCATTGTGTCGTTATGGGCGTAGGCACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTG  >  Exported/1837411‑1837547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: