| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,864,319 | C→T | 15.6% | intergenic (+66/+82) | ygcP → / ← ygcQ | predicted anti‑terminator regulatory protein/predicted flavoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,864,319 | 0 | C | T | 15.6% | 332.3 / 44.2 | 141 | intergenic (+66/+82) | ygcP/ygcQ | predicted anti‑terminator regulatory protein/predicted flavoprotein |
| Reads supporting (aligned to +/- strand): ref base C (71/48); new base T (8/14); total (79/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.05e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.27e-02 | |||||||||||
Only 100 of 144 total aligned reads displayed.
ATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCA > Exported/1864258‑1864388 | tcctgatttagcgtgattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGa > 1:1080715‑M2/60‑71 (MQ=255) gcctgatttagcgtgattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGa > 1:5118810‑M2/60‑71 (MQ=255) gcctgatttagcgtgattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGa > 1:4696563‑M2/60‑71 (MQ=255) tCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTg < 1:4515285/63‑1 (MQ=255) tCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTg < 1:3037523/63‑1 (MQ=255) ctgatttagcgtgattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGc > 1:3826488‑M2/58‑60 (MQ=255) ctgatttagcgtgattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACg > 1:5546817‑M2/58‑71 (MQ=255) ctgatttagcgtgattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACg > 1:3585021‑M2/58‑71 (MQ=255) tgatttagcgtgattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATg < 1:3876066‑M2/9‑1 (MQ=255) cGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGc > 1:2154180/1‑71 (MQ=255) cGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGc > 1:1839906/1‑71 (MQ=255) tAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCtt < 1:2512977/71‑1 (MQ=255) atttagcgtgattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCg > 1:754138‑M2/55‑65 (MQ=255) atttagcgtgattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCt < 1:2089850‑M2/16‑1 (MQ=255) atttagcgtgattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCt < 1:2082957‑M2/16‑1 (MQ=255) atttagcgtgattttgtaggtcggataaggcgtttacgccgcatccgacatcaaCGCCTGATGCGa > 1:3391076‑M2/55‑66 (MQ=255) atttagcgtgattttgtaggtcggataaggcgtttacgccgcatccgacatcaaCGCCTGATGCGa > 1:3384337‑M2/55‑66 (MQ=255) tttagcgtgattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGa > 1:462860‑M2/54‑65 (MQ=255) tttagcgtgattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCtt > 1:3968611‑M2/54‑70 (MQ=255) tttagcgtgattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCtt > 1:2920001‑M2/54‑70 (MQ=255) tttagcgtgattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCtt > 1:2446982‑M2/54‑70 (MQ=255) tttagcgtgattttgtaggtcggataaggcgtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTg < 1:4873076‑M2/18‑1 (MQ=255) aaCCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCt < 1:1281067/58‑1 (MQ=255) ttagcgtgattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACgcttaa > 1:4081563‑M2/53‑69 (MQ=255) ttagcgtgattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACgcttaa > 1:2207788‑M2/53‑69 (MQ=255) aCCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGa > 1:2535854/1‑71 (MQ=255) aCCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGa > 1:3515112/1‑71 (MQ=255) aCCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGa > 1:2220077/1‑71 (MQ=255) aTTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGAcgcg > 1:4217753/1‑71 (MQ=255) tgattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTc < 1:2919820‑M2/25‑1 (MQ=255) tgattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTc > 1:1472609‑M2/47‑71 (MQ=255) tgattttgtaggtcggataaggcgtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTc < 1:2232205‑M2/25‑1 (MQ=255) gattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTc > 1:4360767‑M2/46‑70 (MQ=255) gAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGa > 1:4094521/1‑64 (MQ=255) gAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCt > 1:448279/1‑71 (MQ=255) gAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCt > 1:1880729/1‑71 (MQ=255) attttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCtt < 1:3698561‑M2/27‑1 (MQ=255) attttgtaggtcggataaggcgtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCtt < 1:4347264‑M2/27‑1 (MQ=255) attttgtaggtcggataaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCtt < 1:5355782‑M2/27‑1 (MQ=255) aaTTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCt < 1:5363501/60‑1 (MQ=255) ttttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACgcttaa > 1:670278‑M2/44‑60 (MQ=255) ttttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCtt < 1:3316933‑M2/27‑1 (MQ=255) ttttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTa < 1:5462559‑M2/28‑1 (MQ=255) ttttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTa < 1:673634‑M2/28‑1 (MQ=255) tttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTAt > 1:224601‑M2/43‑71 (MQ=255) ttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTAt < 1:1293882‑M2/29‑1 (MQ=255) ttgtaggtcggataaggcgtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCTTAt < 1:4663289‑M2/29‑1 (MQ=255) tgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATc < 1:1148611‑M2/30‑1 (MQ=255) tgtaggtcggataaggcgtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCTTAt > 1:3133308‑M2/41‑69 (MQ=255) tgtaggtcggataaggcgtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCTTAt > 1:3968541‑M2/41‑69 (MQ=255) tgtaggtcggataaggcgtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCTTATc < 1:653082‑M2/30‑1 (MQ=255) tgtaggtcggataaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCTTG‑C‑CGTCTTATCAg < 1:1112508‑M2/30‑1 (MQ=255) tgtaggtcggacaaggcgtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCTTAt > 1:1598038‑M2/41‑69 (MQ=255) tGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTg > 1:2004056/1‑58 (MQ=255) gtaggtcggataaggcgtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCTTATCa > 1:4427802‑M2/40‑70 (MQ=255) gtaggtcggataaggcgtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCTTATCAg > 1:2185675‑M2/40‑71 (MQ=255) gtaggtcggataaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCTTATCa < 1:2792159‑M2/31‑1 (MQ=255) taggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATCAg < 1:41480‑M2/32‑1 (MQ=255) taggtcggataaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCTTATCAg < 1:2746008‑M2/32‑1 (MQ=255) ttGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGcc > 1:2599483/1‑71 (MQ=255) ggtcggataaggcgtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc < 1:4881947‑M2/35‑1 (MQ=255) ggtcggataaggcgtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc < 1:3460856‑M2/35‑1 (MQ=255) ggtcggataaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc < 1:4076012‑M2/35‑1 (MQ=255) tGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCtt < 1:1603926/52‑1 (MQ=255) tGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCtt < 1:229607/52‑1 (MQ=255) gATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGa > 1:1372786/1‑53 (MQ=255) cggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTAc > 1:4184183‑M2/34‑71 (MQ=255) cggataaggcgtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCt > 1:2936739‑M2/34‑59 (MQ=255) cggataaggcgtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCt > 1:146894‑M2/34‑59 (MQ=255) aTTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCa > 1:1424992/1‑64 (MQ=255) tttGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTAc < 1:5283338/70‑1 (MQ=255) tAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGt < 1:324661/70‑1 (MQ=255) aaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCtacgc > 1:530452‑M2/29‑66 (MQ=255) aaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCtacgc > 1:2587739‑M2/29‑66 (MQ=255) gcgtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCtat > 1:1068605‑M2/26‑62 (MQ=255) gtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCt < 1:5605964‑M2/36‑1 (MQ=255) gtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCt < 1:2674530‑M2/36‑1 (MQ=255) gtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCt < 1:4449442‑M2/36‑1 (MQ=255) tttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAAcgc < 1:3716734‑M2/22‑1 (MQ=255) tttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAAcgc < 1:244242‑M2/22‑1 (MQ=255) cACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGc > 1:4758146/1‑71 (MQ=255) tacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCTTATc > 1:3476384‑M2/21‑50 (MQ=255) tacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCt > 1:116615‑M2/21‑56 (MQ=255) gCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAg < 1:4450612/71‑1 (MQ=255) gccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCTTAt > 1:4430917‑M2/18‑46 (MQ=255) gccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTa > 1:142877‑M2/18‑54 (MQ=255) cacaTCCGACATTCAGCGCCTGATGCGACGCTTGACGCGt < 1:3751004/40‑1 (MQ=255) cacaTCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGACCCCAGGCCAGaa > 1:711889/1‑71 (MQ=255) cgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATCAg > 1:5136755‑M2/16‑47 (MQ=255) tCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCg > 1:3269075/1‑70 (MQ=255) ccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc < 1:4514583‑M2/35‑1 (MQ=255) aCATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAgg > 1:40769/1‑70 (MQ=255) aCATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAgg > 1:3611824/1‑70 (MQ=255) aCATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAgg > 1:5500973/1‑70 (MQ=255) acatcaaCGCCTGATGCGACGCTTAACGCGTCTTAt < 1:1674856‑M2/29‑1 (MQ=255) ttCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCa < 1:3633859/52‑1 (MQ=255) ttCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAg < 1:1916679/70‑1 (MQ=255) cAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGat > 1:4839842/1‑71 (MQ=255) gcgcCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTa > 1:3338958/1‑61 (MQ=255) gcgcCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGatca < 1:3661102/71‑1 (MQ=255) | ATAATCGTAACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCA > Exported/1864258‑1864388 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |