Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,864,337 A→C 32.0% intergenic (+84/+64) ygcP → / ← ygcQ predicted anti‑terminator regulatory protein/predicted flavoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,864,3370AC32.0% 80.6 / 97.9 108intergenic (+84/+64)ygcP/ygcQpredicted anti‑terminator regulatory protein/predicted flavoprotein
Reads supporting (aligned to +/- strand):  ref base A (36/37);  new base C (16/18);  total (52/56)
Fisher's exact test for biased strand distribution p-value = 8.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-02

Only 100 of 114 total aligned reads displayed.

ACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGT  >  Exported/1864267‑1864401
                                                                      |                                                                
aCCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGa                                                                  >  1:2535854/1‑71 (MQ=255)
aCCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGa                                                                  >  1:2220077/1‑71 (MQ=255)
aCCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGa                                                                  >  1:3515112/1‑71 (MQ=255)
 ccAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGAc                                                                 <  1:3375874/71‑1 (MQ=255)
    aTTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGAcgcg                                                              >  1:4217753/1‑71 (MQ=255)
      tgattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTc                                                            >  1:1472609‑M2/47‑71 (MQ=255)
      tGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTc                                                            >  1:5078491/1‑71 (MQ=255)
       gattttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTc                                                            >  1:4360767‑M2/46‑70 (MQ=255)
       gattttgtaggtcggataaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCt                                                           >  1:455065‑M2/46‑71 (MQ=255)
       gAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGa                                                                  >  1:4094521/1‑64 (MQ=255)
       gAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCt                                                           >  1:448279/1‑71 (MQ=255)
       gAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCt                                                           >  1:1880729/1‑71 (MQ=255)
        attttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCtt                                                          <  1:3698561‑M2/27‑1 (MQ=255)
        attttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCtt                                                          <  1:1652032‑M2/27‑1 (MQ=255)
        attttgtaggtcggataaggcgtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCtt                                                          <  1:4347264‑M2/27‑1 (MQ=255)
        attttgtaggtcggataaggcgtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCtt                                                          <  1:701828‑M2/27‑1 (MQ=255)
        attttgtaggtcggataaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCtt                                                          <  1:5355782‑M2/27‑1 (MQ=255)
         ttttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCtt                                                          <  1:3316933‑M2/27‑1 (MQ=255)
         ttttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTa                                                         <  1:5462559‑M2/28‑1 (MQ=255)
         ttttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTa                                                         <  1:673634‑M2/28‑1 (MQ=255)
         ttttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTa                                                         >  1:2930468‑M2/44‑71 (MQ=255)
          tttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTAt                                                        >  1:224601‑M2/43‑71 (MQ=255)
           ttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTAt                                                        <  1:4676571‑M2/29‑1 (MQ=255)
           ttgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTAt                                                        <  1:1293882‑M2/29‑1 (MQ=255)
           ttgtaggtcggataaggcgtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCTTAt                                                        <  1:4663289‑M2/29‑1 (MQ=255)
            tgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATc                                                       <  1:1148611‑M2/30‑1 (MQ=255)
            tgtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATc                                                       <  1:2192035‑M2/30‑1 (MQ=255)
            tgtaggtcggataaggcgtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCTTAt                                                        >  1:3133308‑M2/41‑69 (MQ=255)
            tgtaggtcggataaggcgtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCTTAt                                                        >  1:3968541‑M2/41‑69 (MQ=255)
            tgtaggtcggataaggcgtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCTTATc                                                       <  1:653082‑M2/30‑1 (MQ=255)
            tgtaggtcggataaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGTCTTATCAg                                                     <  1:1112508‑M2/30‑1 (MQ=255)
            tgtaggtcggacaaggcgtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCTTAt                                                        >  1:1598038‑M2/41‑69 (MQ=255)
             gtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTa                                                         >  1:3689311‑M2/40‑67 (MQ=255)
             gtaggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTa                                                         >  1:2505612‑M2/40‑67 (MQ=255)
             gtaggtcggataaggcgtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCTTATCa                                                      >  1:4427802‑M2/40‑70 (MQ=255)
             gtaggtcggataaggcgtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCTTATCAg                                                     >  1:2185675‑M2/40‑71 (MQ=255)
             gtaggtcggataaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCTTATCa                                                      <  1:2792159‑M2/31‑1 (MQ=255)
              taggtcggataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATCAg                                                     <  1:41480‑M2/32‑1 (MQ=255)
              taggtcggataaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCTTATCAg                                                     <  1:2746008‑M2/32‑1 (MQ=255)
                ttGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGcc                                                  >  1:2015977/1‑71 (MQ=255)
                ggtcggataaggcgtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                  <  1:3460856‑M2/35‑1 (MQ=255)
                ggtcggataaggcgtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                  <  1:4881947‑M2/35‑1 (MQ=255)
                ggtcggataaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                  <  1:5406054‑M2/35‑1 (MQ=255)
                ggtcggataaggcgtttacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                  <  1:4076012‑M2/35‑1 (MQ=255)
                  gATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGa                                                                  >  1:1372786/1‑53 (MQ=255)
                   cggataaggcgtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCt                                                           >  1:2936739‑M2/34‑59 (MQ=255)
                   cggataaggcgtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCt                                                           >  1:146894‑M2/34‑59 (MQ=255)
                   cggataaggcgtttacgccgcatccgacatcaaCGCCTGATGAGACGCTTGCCGCGTCTTATc                                                       >  1:146277‑M2/34‑63 (MQ=255)
                   aTTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCa                                                      >  1:5325027/1‑64 (MQ=255)
                   aTTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCa                                                      >  1:1424992/1‑64 (MQ=255)
                    tttGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTAc                                               <  1:5283338/70‑1 (MQ=255)
                     gataaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCtacgc                                             <  1:3081113‑M2/40‑3 (MQ=255)
                       taaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCtacgc                                             >  1:284181‑M2/30‑67 (MQ=255)
                        tAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGt                                           <  1:3632107/70‑1 (MQ=255)
                        tAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGt                                           <  1:324661/70‑1 (MQ=255)
                        aaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCtacgc                                             >  1:257903‑M2/29‑66 (MQ=255)
                        aaggcgtttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCtacgc                                             >  1:2587739‑M2/29‑66 (MQ=255)
                           gcgtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCtat                                               >  1:1068605‑M2/26‑62 (MQ=255)
                             gtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCt                                                 <  1:1522535‑M2/36‑1 (MQ=255)
                             gtttacgccgcatccgacatcaaTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCt                                                 <  1:2674530‑M2/36‑1 (MQ=255)
                              tttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAAcgc                                                               <  1:3716734‑M2/22‑1 (MQ=255)
                              tttatgccgcatccgacatcaaCGCCTGATGCGACGCTTAAcgc                                                               <  1:244242‑M2/22‑1 (MQ=255)
                                tacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCTTATc                                                       >  1:3476384‑M2/21‑50 (MQ=255)
                                tacgccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCt                                                 >  1:116615‑M2/21‑56 (MQ=255)
                                 acgccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCTTATCa                                                      >  1:4434130‑M2/20‑50 (MQ=255)
                                  gCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAg                                <  1:4450612/71‑1 (MQ=255)
                                   gccgcatccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCTTAt                                                        >  1:4430917‑M2/18‑46 (MQ=255)
                                    cacaTCCGACATTCAGCGCCTGATGCGACGCTTGACGCGt                                                             <  1:3751004/40‑1 (MQ=255)
                                    cacaTCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGaa                              >  1:1127775/1‑71 (MQ=255)
                                    cacaTCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGACCCCAGGCCAGaa                              >  1:711889/1‑71 (MQ=255)
                                     cgcatccgacatcaaCGCCTGATGCGACGCTTAACGCGTCTTATCAg                                                     >  1:5136755‑M2/16‑47 (MQ=255)
                                       aTCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATc                                                       >  1:2745560/1‑43 (MQ=255)
                                        tCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCg                           >  1:3269075/1‑70 (MQ=255)
                                         tccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCTTATc                                                       <  1:4456975‑M2/30‑1 (MQ=255)
                                         ccGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCg                           >  1:2345368/1‑69 (MQ=255)
                                          ccgacatcaaCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                  <  1:4514583‑M2/35‑1 (MQ=255)
                                            aCATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAgg                       >  1:40769/1‑70 (MQ=255)
                                            aCATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAgg                       >  1:5500973/1‑70 (MQ=255)
                                               ttCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCa                                      <  1:3633859/52‑1 (MQ=255)
                                               ttCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAg                    <  1:1916679/70‑1 (MQ=255)
                                                 cAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGat                 >  1:4839842/1‑71 (MQ=255)
                                                   gcgcCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCg                           <  1:4080946/59‑1 (MQ=255)
                                                   gcgcCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTa                         >  1:3338958/1‑61 (MQ=255)
                                                   gcgcCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGatca               <  1:3661102/71‑1 (MQ=255)
                                                         gATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGaa                              <  1:4016461/50‑1 (MQ=255)
                                                         gATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGc                      <  1:1991609/58‑1 (MQ=255)
                                                          aTGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGatcat              >  1:631000/1‑65 (MQ=255)
                                                          aTGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGc         <  1:3136347/70‑1 (MQ=255)
                                                             cGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAaca     <  1:4918419/71‑1 (MQ=255)
                                                                cGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGaa                              <  1:4197581/43‑1 (MQ=255)
                                                                cGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGt  <  1:2695570/71‑1 (MQ=255)
                                                                cGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGt  <  1:5314842/71‑1 (MQ=255)
                                                                cGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGt  <  1:1470874/71‑1 (MQ=255)
                                                                 gCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCgg          >  1:2813409/1‑62 (MQ=255)
                                                                 gCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCgg          >  1:5004839/1‑62 (MQ=255)
                                                                   ttGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACg   >  1:3448423/1‑67 (MQ=255)
                                                                    tGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAg                    <  1:5368641/49‑1 (MQ=255)
                                                                    tGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAaca     >  1:687577/1‑64 (MQ=255)
                                                                     gACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCa        <  1:5671956/60‑1 (MQ=255)
                                                                      aCGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGatca               <  1:3776696/52‑1 (MQ=255)
                                                                      |                                                                
ACCAATTGAATTTGGTTTGATTTGTAGGCCGCACGCCACATCCGACATTCAGCGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCCCCAGGCCAGAACCGTAGGCAGGATCATCCGGCAACACGT  >  Exported/1864267‑1864401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: