Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 310,791 T→G 8.5% K694N (AAA→AAC) ‡ acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported310,7910TG 8.5% 138.7 / 7.5 59K694N (AAA→AAC) ‡acrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base T (23/31);  new base G (3/2);  total (26/33)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01

CGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTT  >  Exported/310723‑310855
                                                                    |                                                                
cGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGttt                                                                <  1:4757332/71‑1 (MQ=255)
cGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGttt                                                                <  1:3711164/71‑1 (MQ=255)
 gTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGtttt                                                               <  1:5607459/71‑1 (MQ=255)
 gTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGtttt                                                               <  1:2909431/71‑1 (MQ=255)
    cGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGtttt                                                               <  1:4161674/68‑1 (MQ=255)
    cGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCg                                                            >  1:3586773/1‑71 (MQ=255)
    cGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCg                                                            >  1:3162654/1‑71 (MQ=255)
     gCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGt                                                           <  1:1106219/71‑1 (MQ=255)
     gCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGt                                                           <  1:5023481/71‑1 (MQ=255)
     gCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGt                                                           <  1:4482940/71‑1 (MQ=255)
         gTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGAcc                                                       <  1:295943/71‑1 (MQ=255)
           cAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCGTGAGTCAGTTTTTCGTGAcc                                                       >  1:280878/1‑69 (MQ=255)
           cAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGAcc                                                       <  1:3969444/69‑1 (MQ=255)
           cAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGAcc                                                       >  1:4977237/1‑69 (MQ=255)
              cATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGc                                                  <  1:83119/71‑1 (MQ=255)
              cATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGc                                                  <  1:5482814/71‑1 (MQ=255)
                tatCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCa                                                <  1:194227/71‑1 (MQ=255)
                       gTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGAcc                                                       <  1:5345261/57‑1 (MQ=255)
                          cTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCaa                                      <  1:4248127/71‑1 (MQ=255)
                          cTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCaa                                      >  1:5678351/1‑71 (MQ=255)
                          cTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCa                                       <  1:2166150/70‑1 (MQ=255)
                          cTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCa                                       <  1:4569373/70‑1 (MQ=255)
                            tCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGttt                                                                <  1:3740370/43‑1 (MQ=255)
                            tCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGttt                                                                <  1:3167609/43‑1 (MQ=255)
                            tCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGttt                                                                <  1:1571864/43‑1 (MQ=255)
                             cGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAAt                                     >  1:2705732/1‑69 (MQ=255)
                             cGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAAt                                     >  1:1054075/1‑69 (MQ=255)
                             cGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAAt                                     >  1:1785531/1‑69 (MQ=255)
                               ctgctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTc                                                             <  1:4555066/43‑1 (MQ=255)
                                tgctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCt                                >  1:3442614/1‑71 (MQ=255)
                                 gctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGt                                                           >  1:1755266/1‑43 (MQ=255)
                                 gctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCt                                >  1:1410835/1‑70 (MQ=255)
                                   ttCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtca                              >  1:2339927/1‑70 (MQ=255)
                                   ttCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtca                              >  1:4169904/1‑70 (MQ=255)
                                    tCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGcc                                             >  1:1967700/1‑54 (MQ=255)
                                     cTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGAc                                                        <  1:1587239/42‑1 (MQ=255)
                                     cTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaa                            <  1:1220595/70‑1 (MQ=255)
                                     cTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaa                             <  1:817166/69‑1 (MQ=255)
                                      tGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCg                                                            >  1:1733112/1‑37 (MQ=255)
                                      tGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCg                                                            >  1:3649713/1‑37 (MQ=255)
                                      tGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGAcc                                                       <  1:1351816/42‑1 (MQ=255)
                                      tGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGAcc                                                       <  1:70925/42‑1 (MQ=255)
                                      tGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGAcc                                                       <  1:5243633/42‑1 (MQ=255)
                                       gcaagcaaCTTGTTACGCGCCTGAGTCAGTTTTTCGTGACCa                                                      >  1:1807289/1‑42 (MQ=255)
                                       gcaagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCa                                                      >  1:2489477/1‑42 (MQ=255)
                                       ccaagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtc                         <  1:1626494/70‑1 (MQ=255)
                                         aagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtcga                       <  1:999838/71‑3 (MQ=255)
                                         aagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaa                       <  1:1283897/71‑1 (MQ=255)
                                         aagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaa                       <  1:2247475/71‑1 (MQ=255)
                                          agcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtcga                       >  1:1523076/1‑68 (MQ=255)
                                           gcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtcgaaa                     >  1:4088067/1‑70 (MQ=255)
                                           gcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtcgaaa                     >  1:1711755/1‑70 (MQ=255)
                                           gcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaaag                     >  1:425769/1‑71 (MQ=255)
                                            caaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGc                    >  1:4575506/1‑71 (MQ=255)
                                            caaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGc                    >  1:2708401/1‑71 (MQ=255)
                                             aaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc                   <  1:5341281/71‑1 (MQ=255)
                                               cTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTg                                           <  1:5583754/45‑1 (MQ=255)
                                               cTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAAt                                     >  1:436058/1‑51 (MQ=255)
                                                  gTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaa                             <  1:2018286/56‑1 (MQ=255)
                                                  gTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaa                             <  1:835662/56‑1 (MQ=255)
                                                  gTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTg              <  1:4797019/71‑1 (MQ=255)
                                                  gTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTg              <  1:4700272/71‑1 (MQ=255)
                                                    tACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTg              >  1:4778008/1‑69 (MQ=255)
                                                          ccTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTAccc      <  1:577185/71‑1 (MQ=255)
                                                          ccTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTAccc      <  1:3974914/71‑1 (MQ=255)
                                                              aGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGtt  <  1:2198278/71‑1 (MQ=255)
                                                                   gTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtc                               <  1:3457405/37‑1 (MQ=255)
                                                                    tttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc                   <  1:4875957/48‑1 (MQ=255)
                                                                    tttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc                   <  1:3020273/48‑1 (MQ=255)
                                                                    tttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc                   <  1:2744478/48‑1 (MQ=255)
                                                                    tttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc                   <  1:383746/48‑1 (MQ=255)
                                                                    tttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTg              <  1:1198118/53‑1 (MQ=255)
                                                                    tttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGc             <  1:1731220/54‑1 (MQ=255)
                                                                    |                                                                
CGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTT  >  Exported/310723‑310855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: