| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,929,605 | G→A | 6.0% | L231F (CTT→TTT) | kduD ← | 2‑deoxy‑D‑gluconate 3‑dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,929,605 | 0 | G | A | 6.0% | 301.4 / 5.8 | 100 | L231F (CTT→TTT) | kduD | 2‑deoxy‑D‑gluconate 3‑dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (50/44); new base A (2/4); total (52/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
Only 100 of 114 total aligned reads displayed.
TAACGCGCCAGCCAACCGCCATCCACGGCAATGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGAACACTATCGGCCCCATCAGGTCACTCGGCAGTCCCCAACGACCAGCTGGAATGCGGTCGAGAATTTC > Exported/1929535‑1929674 | tAACGCGCCAGCCAACCGCCATCCACGGCAATGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAg > 1:2134799/1‑71 (MQ=255) aaCGCGCCAGCCAACCGCCATCCACGGCAATGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAg > 1:586318/1‑70 (MQ=255) aCGCGCCAGCCAACCGCCATCCACGGCAATGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAgg < 1:4102871/70‑1 (MQ=255) aCGCGCCAGCCAACCGCCATCCACGGCAATGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAgg > 1:250069/1‑70 (MQ=255) cgcgCCAGCCAACCGCCATCCACGGCAATGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGaa < 1:1224942/71‑1 (MQ=255) cgcgCCAGCCAACCGCCATCCACGGCAATGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGaa > 1:3487666/1‑71 (MQ=255) gcgcCAGCCAACCGCCATCCACGGCAATGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGaa > 1:3173646/1‑70 (MQ=255) ccagccaACCGCCATCCACGGCAATGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGAACACt < 1:2647156/71‑1 (MQ=255) cagccaACCGCCATCCACGGCAATGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGAACACTa < 1:1166026/71‑1 (MQ=255) cagccaACCGCCATCCACGGCAATGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGAACACTa < 1:3961469/71‑1 (MQ=255) cagccaACCGCCATCCACGGCAATGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGAACACTa < 1:3146033/71‑1 (MQ=255) agccaACCGCCATCCACGGCAATGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGAACACTAt > 1:1083504/1‑71 (MQ=255) agccaACCGCCATCCACGGCAATGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGAACACTAt < 1:3749138/71‑1 (MQ=255) ccaACCGCCATCCACGG‑AATGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGAACACTATCgg > 1:1716748/1‑71 (MQ=255) caACCGCCATCCACGGCAATGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAg > 1:332854/1‑59 (MQ=255) caACCGCCATCCACGGCAATGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAg > 1:2245196/1‑59 (MQ=255) aCCGCCATCCACGGCAATGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGAac < 1:4786833/61‑1 (MQ=255) cGCCATCCACGGCAATGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGAACACTATCGGccc > 1:5228500/1‑70 (MQ=255) cATCCACGGCAATGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGAACACTATCGGCCCCAt > 1:4404602/1‑70 (MQ=255) cATCCACGGCAATGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGAACACTATCGGCCCCATc < 1:1066377/71‑1 (MQ=255) aTCCACGGCAATGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGaa < 1:4592249/54‑1 (MQ=255) aTCCACGGCAATGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGaa < 1:414885/54‑1 (MQ=255) tCCACGGCAATGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAgg < 1:5492141/51‑1 (MQ=255) tCCACGGCAATGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGAACACTATCGGcc < 1:4587580/64‑1 (MQ=255) cACGGCAATGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGaa > 1:1335734/1‑51 (MQ=255) cACGGCAATGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGAACACTATCGGCCCCATCAGGt > 1:1229952/1‑71 (MQ=255) aCGGCAATGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGAACACt < 1:4628175/54‑1 (MQ=255) aCGGCAATGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGAACACt < 1:1639975/54‑1 (MQ=255) aCGGCAATGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGAACACTATCGGCCCCATCAGGt > 1:5007193/1‑70 (MQ=255) cGGCAATGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGAACACTATCGGCCCCATCAGGTc > 1:3911095/1‑70 (MQ=255) cAATGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGAACACTATc > 1:5160420/1‑53 (MQ=255) cAATGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGAACACTATc > 1:271547/1‑53 (MQ=255) tGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAgg < 1:5492284/41‑1 (MQ=255) tGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAgg < 1:2378908/41‑1 (MQ=255) ggTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGaa > 1:3561399/1‑42 (MQ=255) ggTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGAACACTATCGGCCCCATCAGGTCACTCGGc < 1:3385211/70‑1 (MQ=255) gTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGAACACTATCGGccc > 1:5143555/1‑53 (MQ=255) gTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGAACACTATCGGccc > 1:1121454/1‑53 (MQ=255) gTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGAACACTATCGGCCCCAt < 1:2926657/56‑1 (MQ=255) gTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGAACACTATCGGCCCCAt < 1:2964486/56‑1 (MQ=255) gTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGAACACTATCGGCCCCAt < 1:3501145/56‑1 (MQ=255) gTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGAACACTATCGGCCCCATCAGGTCACTCGGCAg > 1:5086931/1‑71 (MQ=255) gTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGAACACTATCGGCCCCATCAGGTCACTCGGCAg < 1:719805/71‑1 (MQ=255) tataACCATTCACATAATCTGAAGCGCTGGAGGCAAGGAACACTATCGGCCCCATCAGGTCACTCGGCAGt < 1:1804808/71‑1 (MQ=255) ataACCATTCACATAATCTGAAGCGCTGGAGGCAAGGAACACTATCGGCCCCATCAGGTCACTCGGCAGTc > 1:2961832/1‑71 (MQ=255) taACCATTCACATAATCTGAAGCGCTGGAGGCAAAGAACACt < 1:2571360/42‑1 (MQ=255) taACCATTCACATAATCTGAAGCGCTGGAGGCAAAGAACACt < 1:3979800/42‑1 (MQ=255) taACCATTCACATAATCTGAAGCGCTGGAGGCAAAGAACACt < 1:3260167/42‑1 (MQ=255) taACCATTCACATAATCTGAAGCGCTGGAGGCAAAGAACACt < 1:866147/42‑1 (MQ=255) aCCATTCACATAATCTGAAGCGCTGGAGGCAAGGAACACt < 1:3013580/40‑1 (MQ=255) aCCATTCACATAATCTGAAGCGCTGGAGGCAAGGAACACt < 1:1994162/40‑1 (MQ=255) aCCATTCACATAATCTGAAGCGCTGGAGGCAAAGAACACTAt > 1:4965146/1‑42 (MQ=255) ccATTCACATAATCTGAAGCGCTGGAGGCAAGGAACACTATCGGCCCCATCAGGTCACTCGGCa > 1:4817468/1‑64 (MQ=255) ccATTCACATAATCTGAAGCGCTGGAGGCAAGGAACACTATCGGCCCCATCAGGTCACTCGGCAg > 1:4426676/1‑65 (MQ=255) ccATTCACATAATCTGAAGCGCTGGAGGCAAGGAACACTATCGGCCCCATCAGGTCACTCGGCAGTcccc > 1:3696547/1‑70 (MQ=255) tCACATAATCTGAAGCGCTGGAGGCAAGGAACACTAt > 1:2503391/1‑37 (MQ=255) cacaTAATCTGAAGCGCTGGAGGCAAGGAACACTATCGGCCCCATc > 1:25488/1‑46 (MQ=255) cacaTAATCTGAAGCGCTGGAGGCAAGGAACACTATCGGCCCCATCAGGTCACTCGGCAGt > 1:104281/1‑61 (MQ=255) cacaTAATCTGAAGCGCTGGAGGCAAGGAACACTATCGGCCCCATCAGGTCACTCGGCAGt > 1:4109366/1‑61 (MQ=255) cacaTAATCTGAAGCGCTGGAGGCAAGGAACACTATCGGCCCCATCAGGTCACTCGGCAGt > 1:5023595/1‑61 (MQ=255) cacaTAATCTGAAGCGCTGGAGGCAAGGAACACTATCGGCCCCATCAGGTCACTCGGCAGTCCCCAACGAc > 1:750128/1‑71 (MQ=255) aTAATCTGAAGCGCTGGAGGCAAGGAACACTATCGGCCCCATCAGGTCACTc < 1:456500/52‑1 (MQ=255) aTAATCTGAAGCGCTGGAGGCAAGGAACACTATCGGCCCCATCAGGTCACTCGGCAGTCCCCAACGACCAg > 1:3664085/1‑71 (MQ=255) tAATCTGAAGCGCTTGAGGCAAGGAACACTATCGGCCCCATCa > 1:1749611/1‑43 (MQ=255) tAATCTGAAGCGCTGGTGGCAAGGAACACTATCGGCCCCATc > 1:3332042/1‑42 (MQ=255) tAATCTGAAGCGCTGGAGGCAAGGAACACTATCGGCCCCATCa > 1:1430626/1‑43 (MQ=255) tAATCTGAAGCGCTGGAGGCAAGGAACACTATCGGCCCCATCAGGTCACTCGGCAGTCCCCAACGACCAg < 1:5306119/70‑1 (MQ=255) tAATCTGAAGCGCTGGAGGCAAGGAACACTATCGGCCCCATCAGGTCACTCGGCAGTCCCCAACGACCAg < 1:2337502/70‑1 (MQ=255) tAATCTGAAGCGCTGGAGGCAAGGAACACTATCGGCCCCATCAGGTCACTCGGCAGTCCCCAACGACCAGc > 1:2765184/1‑71 (MQ=255) tAATCTGAAGCGCTGGAGGCAAGGAACACTATCGGCCCCATCAGGTCACTCGGCAGTCCCCAACGACCAGc > 1:820434/1‑71 (MQ=255) aaTCTGAAGCGCTGGAGGCAAGGAACACTATCGGCCCCATCAGGTCACTCGGCAGTCCCCAACGACCAGCt > 1:1170680/1‑71 (MQ=255) aTCTGAAGCGCTGGAGGCAAGGAACACTATCGGCCCCATCAGGTCACTCGGCAGTCCCCAACGACCAg < 1:672723/68‑1 (MQ=255) aTCTGAAGCGCTGGAGGCAAGGAACACTATCGGCCCCATCAGGTCACTCGGCAGTCCCCAACGACCAGCTg > 1:2060676/1‑71 (MQ=255) aTCTGAAGCGCTGGAGGCAAGGAACACTATCGGCCCCATCAGGTCACTCGGCAGTCCCCAACGACCAGCTg > 1:154744/1‑71 (MQ=255) tCTGAAGCGCTGGAGGCAAGGAACACTATCGGCCCCATCAGGTCACTCGGCAGTCCCCAAc < 1:2372247/61‑1 (MQ=255) aaGCGCTGGAGGCAAGGAACACTATCGGCCTCATCAGGTCACTCGGCAGTCCCCAACGACCAGCTgg < 1:4163598/67‑1 (MQ=255) aaGCGCTGGAGGCAAGGAACACTATCGGCCCCATCAGGTCACTCGGCAGTCCCCAACGACCAGCTgg < 1:4553115/67‑1 (MQ=255) aaGCGCTGGAGGCAAGGAACACTATCGGCCCCATCAGGTCACTCGGCAGTCCCCAACGACCAGCTgg < 1:3855109/67‑1 (MQ=255) aaGCGCTGGAGGCAAGGAACACTATCGGCCCCATCAGGTCACTCGGCAGTCCCCAACGACCAGCTgg < 1:3822878/67‑1 (MQ=255) aaGCGCTGGAGGCAAGGAACACTATCGGCCCCATCAGGTCACTCGGCAGTCCCCAACGACCAGCTGGa < 1:345270/68‑1 (MQ=255) aaGCGCTGGAGGCAAGGAACACTATCGGCCCCATCAGGTCACTCGGCAGTCCCCAACGACCAGCTGGa < 1:4726047/68‑1 (MQ=255) aaGCGCTGGAGGCAAGGAACACTATCGGCCCCATCAGGTCACTCGGCAGTCCCCAACGACCAGCTGGa < 1:1914488/68‑1 (MQ=255) cTGGAGGCAAGGAACACTATCGGCCCCATCAGGTCACTCGGCAGTCCGCAACGACCAGCTGGAATg < 1:2544016/66‑1 (MQ=255) cTGGAGGCAAGGAACACTATCGGCCCCATCAGGTCACTCGGCAGTCCCCAACGACCAGCTGGAATGCGGTc > 1:911225/1‑71 (MQ=255) gAGTCAAGGAACACTATCGGCCCCATCAGGTCACTCGGCAGTCCCCAACGACCAGCTGGAATGCGGTCgag < 1:3804119/71‑1 (MQ=255) gAGGCAAGGAACACTATCGGCCCCATCAGGTCACTCGGCAGTCCCCAACGACCAGCTGGAATGCGGTCgag < 1:2210130/71‑1 (MQ=255) ggCAAGGAACACTATCGGCCCCATCAGGTCACTCgg < 1:1774469/36‑1 (MQ=255) ggCAAGGAACACTATCGGCCCCATCAGGTCACTCGGCAg > 1:162205/1‑39 (MQ=255) cAAGGAACACTATCGGCCCCATCAGGTCACTCGGCa > 1:3206354/1‑36 (MQ=255) aaGGAACACTATCGGCCCCATCAGGTCACTCGGCAGTCCCCAACGACCAGCTGGAATGCGGTCga < 1:2048847/65‑1 (MQ=255) aaGGAACACTATCGGCCCCATCAGGTCACTCGGCAGTCCCCAACGACCAGCTGGAATGCGGTCGAGAAttt > 1:4454932/1‑71 (MQ=255) aaGGAACACTATCGGCCCCATCAGGTCACTCGGCAGTCCCCAACGACCAGCTGGAATGCGGTCGAGAAtt > 1:4766731/1‑70 (MQ=255) aaGGAACACTATCGGCCCCATCAGGTCACTCGGCAGTCCCCAACGACCAGCTGGAATGCGGTCGAGAAt > 1:4203447/1‑69 (MQ=255) aaGGAACACTATCGGCCCCATCAGGTCACTCGGCAGTCCCCAACGACCAGCTGGAATGCGGTCGAGAAt > 1:2601213/1‑69 (MQ=255) aGGAACACTATCGGCCCCATCAGGTCACTCGGCAGTCCCCAACGACCAGCTGGAATGCGGTCGAGAATTTc < 1:2319178/71‑1 (MQ=255) ggAACACTATCGGCCCCATCAGGTCACTCGGCAGTCCCCAACGACCa > 1:139561/1‑47 (MQ=255) ggAACACTATCGGCCCCATCAGGTCACTCGGCAGTCCCCAACGACCAGCTgg < 1:3992276/52‑1 (MQ=255) ggAACACTATCGGCCCCATCAGGTCACTCGGCAGTCCCCAACGACCAGCTgg < 1:3878715/52‑1 (MQ=255) ggAACACTATCGGCCCCATCAGGTCACTCGGCAGTCCCCAACGACCAGCTgg < 1:294468/52‑1 (MQ=255) ggAACACTATCGGCCCCATCAGGTCACTCGGCAGTCCCCAACGACCAGCTGGa < 1:4472497/53‑1 (MQ=255) | TAACGCGCCAGCCAACCGCCATCCACGGCAATGGTATAACCATTCACATAATCTGAAGCGCTGGAGGCAAGGAACACTATCGGCCCCATCAGGTCACTCGGCAGTCCCCAACGACCAGCTGGAATGCGGTCGAGAATTTC > Exported/1929535‑1929674 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |