| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,932,952 | C→G | 13.0% | L497L (CTG→CTC) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,932,952 | 0 | C | G | 13.0% | 299.0 / 28.9 | 115 | L497L (CTG→CTC) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base C (59/41); new base G (12/3); total (71/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01 | |||||||||||
Only 100 of 118 total aligned reads displayed.
AACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGAC > Exported/1932885‑1933022 | ccGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATaa > 1:4780566/1‑71 (MQ=255) cGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAAc > 1:5345731/1‑71 (MQ=255) gACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACg < 1:4597807/71‑1 (MQ=255) aCATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGt > 1:3303935/1‑71 (MQ=255) tttGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTcgc < 1:4789907/71‑1 (MQ=255) tGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTcgcg > 1:180530/1‑70 (MQ=255) ggggTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg < 1:3836129/71‑1 (MQ=255) gggTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg > 1:602358/1‑70 (MQ=255) gTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTAt < 1:4277311/71‑1 (MQ=255) gTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGAGGATGGTAt > 1:4475544/1‑71 (MQ=255) ttGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATg > 1:5326697/1‑71 (MQ=255) gtgAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATaa > 1:2871391/1‑53 (MQ=255) gtgAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATaa > 1:297050/1‑53 (MQ=255) gtgAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATa > 1:5685258/1‑52 (MQ=255) gtgAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGc > 1:1432984/1‑71 (MQ=255) gggCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTTTGGCt < 1:4852350/68‑1 (MQ=255) aGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGt < 1:2807755/71‑1 (MQ=255) gCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGtt > 1:3595818/1‑71 (MQ=255) cATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg < 1:2753239/60‑1 (MQ=255) cATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTg > 1:2843988/1‑71 (MQ=255) aTAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACg < 1:193481/49‑1 (MQ=255) aTAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACg < 1:3069515/49‑1 (MQ=255) aTAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACg < 1:1183141/49‑1 (MQ=255) aTAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACg < 1:4083497/49‑1 (MQ=255) tAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTc < 1:1726259/50‑1 (MQ=255) tAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTc < 1:4513658/50‑1 (MQ=255) tAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTc < 1:1589976/50‑1 (MQ=255) aCGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTg < 1:1874147/70‑1 (MQ=255) aCGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTg > 1:1143899/1‑70 (MQ=255) aCGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTg < 1:2485013/70‑1 (MQ=255) aaTGCTTATTTTACCGGACGCATCGCCGGGAACAg < 1:3181138/35‑1 (MQ=255) aaTGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTa > 1:4910924/1‑54 (MQ=255) aTGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTcgcg < 1:5062217/46‑1 (MQ=255) aTGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTcgcg < 1:1955484/46‑1 (MQ=255) aTGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGtt < 1:4830640/61‑1 (MQ=255) aTGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGtt < 1:1341780/61‑1 (MQ=255) tGCTTATTTTACCGGACGCATCGCCGGGAACAGaa < 1:3885960/35‑1 (MQ=255) gCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTgg > 1:4501457/1‑61 (MQ=255) cTTATTTTACCGGACGCATCGCCGGGAACAGAATAACg > 1:4999021/1‑38 (MQ=255) cTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATg > 1:2058451/1‑47 (MQ=255) cTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATg > 1:569442/1‑47 (MQ=255) cTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATg > 1:2407182/1‑47 (MQ=255) cTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCAt < 1:3173011/70‑1 (MQ=255) cTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCAt < 1:287165/70‑1 (MQ=255) cTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCAt < 1:1281469/70‑1 (MQ=255) ttATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACa > 1:3424891/1‑65 (MQ=255) ttATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTa > 1:80001/1‑71 (MQ=255) ttATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTa > 1:2810753/1‑71 (MQ=255) aTTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTa > 1:71809/1‑47 (MQ=255) aTTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTa > 1:2966404/1‑47 (MQ=255) aTTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTAc < 1:4162378/70‑1 (MQ=255) aTTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTAc < 1:1140601/70‑1 (MQ=255) ttttACCGGACGCATCGCCGGGAACAGAATAACGTcgc < 1:4702543/38‑1 (MQ=255) ttttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGTATGGTATGGCTGTTGGTGa > 1:34734/1‑59 (MQ=255) ttttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTa > 1:2451728/1‑46 (MQ=255) ttttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGGTg > 1:723710/1‑55 (MQ=255) tttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTAt > 1:2756339/1‑46 (MQ=255) ttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGtt > 1:5106035/1‑52 (MQ=255) ttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGtt > 1:804596/1‑52 (MQ=255) tACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATAc > 1:4696342/1‑71 (MQ=255) ccGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACg < 1:469845/70‑1 (MQ=255) ccGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACg < 1:5661682/70‑1 (MQ=255) gACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGaa > 1:1195276/1‑52 (MQ=255) gACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTc > 1:4596429/1‑70 (MQ=255) cGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGa > 1:1911385/1‑70 (MQ=255) tCGCCGGTAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTAc < 1:2761608/55‑1 (MQ=255) tCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGt < 1:3926415/39‑1 (MQ=37) tCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGtt < 1:1302562/40‑1 (MQ=255) tCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGa < 1:2358882/45‑1 (MQ=255) tCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTAc < 1:5327225/55‑1 (MQ=255) tCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTAc < 1:4976200/55‑1 (MQ=255) cGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa > 1:4480796/1‑71 (MQ=255) cGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa > 1:214042/1‑71 (MQ=255) cGCCGGGAAGAGAATAACGTCGCGAATAGTATGACTGTTTGTAAACAGCATAATCATTCGGt > 1:4371568/1‑62 (MQ=255) cGCCGGGAAGAGAATAACGTCGCGAATAGTATGACTGTT‑GTAAACAGCATAATCATTCGGt > 1:3371417/1‑61 (MQ=255) gCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCAtt > 1:1258370/1‑56 (MQ=255) gCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa > 1:4613870/1‑70 (MQ=255) gCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa > 1:4542885/1‑70 (MQ=255) gCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa > 1:1121669/1‑70 (MQ=255) gCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa > 1:1549438/1‑70 (MQ=255) gCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACa > 1:3372839/1‑46 (MQ=255) gCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACa > 1:1871851/1‑46 (MQ=255) gCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAAt > 1:596568/1‑71 (MQ=255) gCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAAt > 1:2515203/1‑71 (MQ=255) ccGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAg > 1:5293691/1‑46 (MQ=255) ccGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAg > 1:2576988/1‑46 (MQ=255) ccGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAg > 1:1630853/1‑46 (MQ=255) ccGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCa > 1:2797841/1‑48 (MQ=255) ccGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATa > 1:1964495/1‑56 (MQ=255) ccGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATa > 1:615725/1‑56 (MQ=255) cGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGa > 1:2007391/1‑62 (MQ=255) gggAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCaa > 1:538936/1‑67 (MQ=255) gggAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCaa > 1:166024/1‑67 (MQ=255) gggAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTcc < 1:1912456/71‑1 (MQ=255) ggAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATAcc < 1:1215495/64‑1 (MQ=255) ggAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATAccc < 1:2474814/71‑1 (MQ=255) aaCAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTcc > 1:605765/1‑68 (MQ=255) aCAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGa > 1:4756273/1‑71 (MQ=255) cAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGAc > 1:1415903/1‑71 (MQ=255) cAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGAc > 1:818567/1‑71 (MQ=255) | AACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGAC > Exported/1932885‑1933022 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |