| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,933,024 | T→A | 13.7% | A473A (GCA→GCT) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,933,024 | 0 | T | A | 13.7% | 504.7 / 61.3 | 189 | A473A (GCA→GCT) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base T (97/66); new base A (16/10); total (113/76) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
Only 100 of 209 total aligned reads displayed.
GAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCAC > Exported/1932954‑1933094 | gAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACct > 1:2071754/1‑71 (MQ=255)gAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACct > 1:1768795/1‑71 (MQ=255) aaTAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACct > 1:1113569/1‑70 (MQ=255) tAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgct < 1:5687052/71‑1 (MQ=255) ggATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctg < 1:2438293/60‑1 (MQ=255) ggATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGt < 1:4035160/71‑1 (MQ=255) gATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGt > 1:561937/1‑70 (MQ=255) atagTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTtgg > 1:3781893/4‑65 (MQ=255) tATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACcagcgg < 1:2880802/57‑3 (MQ=255) tATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACcagcgg < 1:3806969/57‑3 (MQ=255) cTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTc < 1:2899381/71‑1 (MQ=255) cTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTc < 1:2287609/71‑1 (MQ=255) cTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTc < 1:5046355/71‑1 (MQ=255) ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTa > 1:4117786/1‑58 (MQ=255) ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTcc < 1:498485/69‑1 (MQ=255) ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTcc < 1:5648073/69‑1 (MQ=255) ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAg < 1:1957555/71‑1 (MQ=255) ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAg > 1:106959/1‑71 (MQ=255) ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAg > 1:249944/1‑71 (MQ=255) ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGAGGTa > 1:163794/1‑58 (MQ=255) tGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCg > 1:837025/1‑71 (MQ=255) tGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCg < 1:594551/71‑1 (MQ=255) aaaCAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAgcgcag > 1:3994867/2‑69 (MQ=255) aaCAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAgcgca > 1:3310219/1‑68 (MQ=12) aCAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTaa < 1:4545840/52‑1 (MQ=255) cAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCAt > 1:5240619/1‑56 (MQ=255) aGCATTACCATACGGTCGATACCAATTCCCAGACct > 1:2953900/1‑36 (MQ=255) aGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCAc > 1:1145874/1‑71 (MQ=255) gCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCg > 1:1486122/1‑65 (MQ=255) tACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTaa > 1:1038074/1‑70 (MQ=255) aCCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCa > 1:4662066/1‑55 (MQ=255) aCCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAAt < 1:711145/70‑1 (MQ=255) aCCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATc > 1:449954/1‑71 (MQ=255) ccATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATc < 1:4741292/70‑1 (MQ=255) ccATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATc < 1:2562783/70‑1 (MQ=255) ccATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCt < 1:3005456/71‑1 (MQ=255) cATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCtt < 1:2191143/71‑1 (MQ=255) cATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCtt < 1:5445100/71‑1 (MQ=255) attcGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTaa > 1:489146/4‑66 (MQ=12) aTACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCtt > 1:4060993/1‑70 (MQ=255) aTACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCtt > 1:4324786/1‑70 (MQ=255) aTACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCtt > 1:4164000/1‑70 (MQ=255) aTACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTc < 1:2721361/71‑1 (MQ=255) tACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTGAGCCATGTTCCAGTGCGGTGACGTAATCTTCa > 1:4605625/1‑71 (MQ=255) tACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCa > 1:5293870/1‑71 (MQ=255) tACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCa > 1:4409699/1‑71 (MQ=255) tACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCa < 1:4850848/71‑1 (MQ=255) tACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCa > 1:3330490/1‑71 (MQ=255) tcGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTcg > 1:1198123/2‑69 (MQ=255) cGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCa > 1:5395705/1‑49 (MQ=255) cGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATc > 1:2249762/1‑71 (MQ=255) cGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATc > 1:3019300/1‑71 (MQ=255) cGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTcgt < 1:1316448/70‑3 (MQ=255) tCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTc < 1:4285014/44‑1 (MQ=255) tCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCg > 1:2556433/1‑69 (MQ=255) tCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCg > 1:2170557/1‑69 (MQ=255) tCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATa < 1:325332/71‑1 (MQ=255) tCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATa < 1:310847/71‑1 (MQ=255) cGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCg > 1:4360687/1‑68 (MQ=255) cGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGAAATCTTCATCg > 1:2891272/1‑68 (MQ=255) gATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAg > 1:344547/1‑70 (MQ=255) gATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAg > 1:364375/1‑70 (MQ=255) gATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAg < 1:4864166/70‑1 (MQ=255) gATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAg < 1:2414069/70‑1 (MQ=255) aTACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGaa < 1:2874446/71‑1 (MQ=255) tACCAATTCCCAGACCTGCTGTCGGCGGTAAGGCATGTTCCAGTGCGGTGa > 1:3754797/1‑51 (MQ=255) tACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGa > 1:1707581/1‑51 (MQ=255) aCCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCa > 1:5337729/1‑61 (MQ=255) aCCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCa > 1:276887/1‑61 (MQ=255) aCCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCAt > 1:2142107/1‑62 (MQ=255) aCCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACa > 1:3031612/1‑71 (MQ=255) ccAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGa < 1:203293/67‑1 (MQ=255) ccAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACAt > 1:787249/1‑71 (MQ=255) cAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGt < 1:3636996/46‑1 (MQ=255) cAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATc > 1:5193060/1‑71 (MQ=255) aaTTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCa > 1:1326279/1‑38 (MQ=255) aTTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGc < 1:4395662/71‑1 (MQ=255) aTTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGc < 1:1390989/71‑1 (MQ=255) ttCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATc < 1:203776/68‑1 (MQ=255) tcacAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCtt > 1:227026/4‑71 (MQ=255) tCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGaa > 1:3671890/1‑63 (MQ=255) tCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCtt > 1:2434104/1‑71 (MQ=255) cccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtc > 1:1049344/1‑71 (MQ=255) ccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcg > 1:4437206/1‑71 (MQ=255) ccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtc < 1:867354/70‑1 (MQ=255) ccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtc < 1:1557915/70‑1 (MQ=255) cAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTc < 1:3379930/51‑1 (MQ=255) cAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTc < 1:3304704/51‑1 (MQ=255) cAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCAt > 1:679348/1‑53 (MQ=255) cAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgt > 1:3588923/1‑71 (MQ=255) aGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtc < 1:2215396/71‑1 (MQ=255) aGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtc < 1:2018189/71‑1 (MQ=255) aGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTtcgtc > 1:3930457/1‑71 (MQ=255) aGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTtcgtc > 1:2688439/1‑71 (MQ=255) aGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTtcgtc < 1:642669/71‑1 (MQ=255) aGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTtcgtc > 1:1584344/1‑71 (MQ=255) aCCTTCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTcgtcgt < 1:5632619/71‑1 (MQ=255) aCCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTa < 1:413791/42‑1 (MQ=255) aCCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTa < 1:1659818/42‑1 (MQ=255) ctgctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCa < 1:3368009/71‑1 (MQ=255) | GAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCAC > Exported/1932954‑1933094 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |