| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,933,057 | G→C | 19.4% | V462V (GTC→GTG) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,933,057 | 0 | G | C | 19.4% | 462.0 / 96.5 | 211 | V462V (GTC→GTG) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (97/73); new base C (27/14); total (124/87) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 211 total aligned reads displayed.
AGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGG > Exported/1932989‑1933123 | aGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCAc > 1:1145874/1‑71 (MQ=255) aTTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTa < 1:222314/71‑1 (MQ=255) tACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTaa > 1:1038074/1‑70 (MQ=255) atcATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATc > 1:1327360/3‑71 (MQ=255) aCCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAAt < 1:711145/70‑1 (MQ=255) aCCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATc > 1:449954/1‑71 (MQ=255) aCCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATc > 1:4053110/1‑71 (MQ=255) ccATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATc < 1:2562783/70‑1 (MQ=255) ccATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATc < 1:2725785/70‑1 (MQ=255) cATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCtt < 1:2191143/71‑1 (MQ=255) attcGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTaa > 1:489146/4‑66 (MQ=12) attcGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTc < 1:4864590/68‑1 (MQ=255) aTACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCtt > 1:4164000/1‑70 (MQ=255) tACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTGAGCCATGTTCCAGTGCGGTGACGTAATCTTCa > 1:4605625/1‑71 (MQ=255) tACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCa > 1:4403023/1‑71 (MQ=255) tcGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTcg > 1:1198123/2‑69 (MQ=255) cGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATc > 1:3019300/1‑71 (MQ=255) cGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATc > 1:2249762/1‑71 (MQ=255) ggTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCg > 1:5649709/1‑71 (MQ=255) tCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCg > 1:3344681/1‑69 (MQ=255) tCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCg > 1:2170557/1‑69 (MQ=255) cGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCg > 1:4360687/1‑68 (MQ=255) cGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGAAATCTTCATCg > 1:2891272/1‑68 (MQ=255) gATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAg > 1:3623786/1‑70 (MQ=255) gATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAg > 1:364375/1‑70 (MQ=255) tACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTc > 1:4768398/1‑61 (MQ=255) tACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGaa > 1:3374853/1‑70 (MQ=255) aCCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCa > 1:276887/1‑61 (MQ=255) aCCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCAt > 1:2142107/1‑62 (MQ=255) ccAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACa < 1:5325187/70‑1 (MQ=255) ccAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACAt > 1:787249/1‑71 (MQ=255) ccAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACAt > 1:243506/1‑71 (MQ=255) ccAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACa < 1:2339575/70‑1 (MQ=255) cAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACa < 1:1720757/69‑1 (MQ=255) cAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATc > 1:5018567/1‑71 (MQ=255) cAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATc > 1:5193060/1‑71 (MQ=255) aTTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGc > 1:4144127/1‑71 (MQ=255) ttCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATc < 1:203776/68‑1 (MQ=255) tCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGaa > 1:3671890/1‑63 (MQ=255) cccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTc < 1:3153636/53‑1 (MQ=255) cccAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTtc < 1:5442157/71‑1 (MQ=255) ccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcg > 1:4437206/1‑71 (MQ=255) ccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcg < 1:2704678/71‑1 (MQ=255) ccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtc < 1:3306023/70‑1 (MQ=255) cAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATc < 1:2074851/48‑1 (MQ=255) cAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATc < 1:5576144/48‑1 (MQ=255) cAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCAt > 1:679348/1‑53 (MQ=255) aGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGa > 1:3312085/1‑58 (MQ=255) aGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtc < 1:2018189/71‑1 (MQ=255) aGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTtcgtc < 1:642669/71‑1 (MQ=255) aGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTtcgtc > 1:1584344/1‑71 (MQ=255) aCCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTa < 1:1659818/42‑1 (MQ=255) tgctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCg < 1:4293080/49‑1 (MQ=255) gTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtc > 1:4344229/1‑62 (MQ=255) gTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgt > 1:5508692/1‑61 (MQ=255) tCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAg < 1:1737174/47‑1 (MQ=255) tCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTg < 1:282302/69‑1 (MQ=255) tCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTg < 1:374490/69‑1 (MQ=255) gcggTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCt < 1:132381/71‑1 (MQ=255) gTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAAc < 1:1524349/44‑1 (MQ=255) gTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtc < 1:1561213/52‑1 (MQ=255) gTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTc < 1:3262295/71‑1 (MQ=255) gTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTc < 1:4807766/71‑1 (MQ=255) gTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTc < 1:4853767/71‑1 (MQ=255) tAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTcgtcgt < 1:863496/56‑1 (MQ=255) tAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTcgtcgt < 1:141426/56‑1 (MQ=255) tAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTcgtcgt < 1:2956462/56‑1 (MQ=255) tAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcg < 1:5427428/71‑1 (MQ=255) aaGCCAAGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgc > 1:2210741/1‑71 (MQ=255) aGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAg > 1:1314586/1‑38 (MQ=255) aGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgt > 1:3367552/1‑51 (MQ=255) aGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgt > 1:4067706/1‑51 (MQ=255) aGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgt > 1:5667727/1‑51 (MQ=255) aGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCAc > 1:5115191/1‑57 (MQ=255) aGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCAc > 1:3049866/1‑57 (MQ=255) cATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACa > 1:3964936/1‑39 (MQ=12) cATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACa > 1:1107454/1‑39 (MQ=12) aTGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCaa > 1:1084495/1‑71 (MQ=255) tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCt < 1:3339026/60‑1 (MQ=255) tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg < 1:1839057/66‑1 (MQ=255) ttCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCt > 1:3531122/1‑71 (MQ=255) ttCCAGCGCAGTCACGTAATCTTCGTCATAGAACa > 1:3405306/1‑35 (MQ=12) ttCCAGCGCAGTCACGTAATCTTCGTCATAGAACa > 1:611364/1‑35 (MQ=12) ttCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCtt > 1:2302530/1‑41 (MQ=16) tCCAGTGCGGTGACGTAATCTTCATCGTAGAACATc > 1:3768674/1‑36 (MQ=255) tCCAGTGCGGTGACGTAATCTTCATCGTAGAACATc > 1:3686878/1‑36 (MQ=255) tCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTg > 1:2664724/1‑71 (MQ=255) ccAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGa > 1:369527/1‑71 (MQ=255) ccAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCttta > 1:693925/1‑58 (MQ=255) ccAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTagc > 1:2511602/1‑61 (MQ=255) ccAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgt > 1:3222538/1‑70 (MQ=255) ccAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgt > 1:4727515/1‑70 (MQ=255) cAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCtt > 1:546473/1‑38 (MQ=255) cAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgtt < 1:2131888/71‑3 (MQ=255) aGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcg > 1:2415427/1‑58 (MQ=255) gTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCa < 1:3920011/44‑1 (MQ=255) gTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATc > 1:2973139/1‑70 (MQ=255) tGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCttt < 1:1386820/54‑1 (MQ=255) gTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATcc < 1:1990504/66‑1 (MQ=255) gTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAgg > 1:2885049/1‑69 (MQ=255) | AGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGG > Exported/1932989‑1933123 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |