| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,933,081 | C→G | 34.1% | A454A (GCG→GCC) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,933,081 | 0 | C | G | 34.1% | 238.3 / 190.4 | 208 | A454A (GCG→GCC) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base C (70/67); new base G (44/27); total (114/94) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
Only 100 of 215 total aligned reads displayed.
CAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCA > Exported/1933011‑1933150 | cAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATc > 1:5193060/1‑71 (MQ=255) aTTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGc > 1:2034544/1‑71 (MQ=255) aTTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGc < 1:1390989/71‑1 (MQ=255) tcacAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCtt > 1:227026/4‑71 (MQ=255) cccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtc > 1:2157477/1‑71 (MQ=255) ccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcg < 1:2704678/71‑1 (MQ=255) ccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcg > 1:4437206/1‑71 (MQ=255) ccAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtc < 1:867354/70‑1 (MQ=255) cAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgt > 1:3588923/1‑71 (MQ=255) aGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTtcgt > 1:4269045/1‑70 (MQ=255) gACCTGCTGTCGGCGGTAAGCCATGTTGCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtcg > 1:2576131/1‑71 (MQ=255) aCCTTCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTcgtcgt < 1:5632619/71‑1 (MQ=255) ccTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCgtcgtc < 1:857411/71‑1 (MQ=255) gctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtc > 1:623682/1‑62 (MQ=255) ctgTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCt > 1:1489893/1‑71 (MQ=255) gTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgtc > 1:4888566/1‑62 (MQ=255) gTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgt > 1:5508692/1‑61 (MQ=255) ggcggTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgt > 1:5431395/1‑58 (MQ=255) ggcggTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCCTCGTCGTCACCTGCGTc > 1:2235179/1‑71 (MQ=255) gcggTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCt < 1:132381/71‑1 (MQ=255) gTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtc < 1:1561213/52‑1 (MQ=255) gTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTc < 1:4807766/71‑1 (MQ=255) gTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTc < 1:3262295/71‑1 (MQ=255) tAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTcgtcgt < 1:141426/56‑1 (MQ=255) tAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTcgtcgt < 1:2332597/56‑1 (MQ=255) tAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcg < 1:5427428/71‑1 (MQ=255) aaGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTcgtcgt > 1:663315/1‑55 (MQ=255) aaGCCAAGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgc > 1:2210741/1‑71 (MQ=255) aGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTtcgt > 1:3367552/1‑51 (MQ=255) aGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCAc > 1:5115191/1‑57 (MQ=255) aGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCAc > 1:3049866/1‑57 (MQ=255) aGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCAc > 1:2749332/1‑57 (MQ=255) ccATGTTCCAGTGCGGTGACATAATCTTCGTCGTAGAACATCGCTTCGTCGTCACCTGCATCt < 1:1116696/63‑1 (MQ=255) ccATATTCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTtc < 1:1729618/47‑1 (MQ=255) cATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGc > 1:2967475/1‑70 (MQ=255) aTGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCaa > 1:1084495/1‑71 (MQ=255) tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCt < 1:3339026/60‑1 (MQ=255) tGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg < 1:3178810/66‑1 (MQ=255) ttCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCt > 1:3531122/1‑71 (MQ=255) ttCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCtttag > 1:1858649/1‑60 (MQ=255) ttCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCtttag > 1:3308526/1‑60 (MQ=255) tCCAGTGCGGTGACGTAATCTTCATCGTAGAACATc > 1:3768674/1‑36 (MQ=255) tCCAGTGCGGTGACGTAATCTTCATCGTAGAACATc > 1:3686878/1‑36 (MQ=255) tCCAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTg > 1:2664724/1‑71 (MQ=255) ccAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGa > 1:369527/1‑71 (MQ=255) ccAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCttta > 1:693925/1‑58 (MQ=255) ccAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTagc > 1:2511602/1‑61 (MQ=255) ccAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgt > 1:3222538/1‑70 (MQ=255) ccAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgt > 1:4727515/1‑70 (MQ=255) cAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCtt > 1:546473/1‑38 (MQ=255) cAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCAc > 1:5659632/1‑47 (MQ=255) cAGCGCAGTCACGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTgtt < 1:2131891/71‑3 (MQ=255) aGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcg > 1:2415427/1‑58 (MQ=255) gTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCa < 1:5014813/44‑1 (MQ=255) gTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCa < 1:3920011/44‑1 (MQ=255) tGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCt < 1:4422532/52‑1 (MQ=255) tGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATc > 1:1347867/1‑69 (MQ=255) gTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATcc < 1:1990504/66‑1 (MQ=255) gTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAgg > 1:2885049/1‑69 (MQ=255) caCGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGaaac > 1:3999744/2‑69 (MQ=255) aCGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCtttag > 1:5537666/1‑47 (MQ=255) aCGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTcctg > 1:1652409/1‑63 (MQ=255) aCGTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACg < 1:1152242/71‑1 (MQ=255) aCGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTg < 1:1086110/59‑1 (MQ=255) aCGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGc < 1:4591973/70‑1 (MQ=255) gTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTagca > 1:4627129/1‑48 (MQ=19) gTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTagca > 1:5189370/1‑48 (MQ=19) gTAATCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGtt < 1:2052306/71‑1 (MQ=255) gTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGc < 1:2108966/68‑1 (MQ=255) aaTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCa < 1:1507352/50‑1 (MQ=255) aTCTTCGTCATAGAACATGGCTTCGTCGTCACCTGCAGCTTTagca > 1:2153076/1‑45 (MQ=16) aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgcg > 1:5333516/1‑71 (MQ=255) aTCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTgc < 1:2946673/70‑1 (MQ=255) cTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGt > 1:5391943/1‑36 (MQ=255) cTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTc > 1:2245386/1‑37 (MQ=255) cTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc > 1:5031790/1‑71 (MQ=255) ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg > 1:4352900/1‑43 (MQ=255) ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg > 1:3642964/1‑43 (MQ=255) ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcgcg > 1:1201384/1‑43 (MQ=255) ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTcg > 1:4335824/1‑41 (MQ=255) ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCt > 1:2063233/1‑71 (MQ=255) ttCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCt > 1:2486038/1‑71 (MQ=255) cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGc < 1:1238746/43‑1 (MQ=255) cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTg < 1:3157432/70‑1 (MQ=255) cATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGa > 1:728693/1‑71 (MQ=255) tcatAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATc < 1:2769368/68‑1 (MQ=255) catAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCt > 1:3431730/3‑71 (MQ=255) atAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGt < 1:557120/57‑1 (MQ=255) atAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCtt > 1:4084607/2‑71 (MQ=255) atAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCt > 1:3321430/2‑70 (MQ=255) tAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGtt < 1:1038443/58‑1 (MQ=255) tAGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGtt < 1:3810730/58‑1 (MQ=255) aGAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTct > 1:3618334/1‑70 (MQ=255) gAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTctg > 1:157900/1‑69 (MQ=255) gAACATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTct < 1:4171349/70‑2 (MQ=255) gAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCcg > 1:4037584/1‑71 (MQ=255) cATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc < 1:5451451/58‑1 (MQ=255) cATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc < 1:2982565/58‑1 (MQ=255) cATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGcc < 1:4142501/58‑1 (MQ=255) cGCTTCGTCGTCACCTGCGTCTTTCGCTGCAACCTGATCCAGGAAGc < 1:1793986/47‑1 (MQ=255) | CAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAATCTTCATCGTAGAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCA > Exported/1933011‑1933150 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |