| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,933,144 | C→T | 37.8% | A433A (GCG→GCA) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,933,144 | 0 | C | T | 37.8% | 137.2 / 161.4 | 164 | A433A (GCG→GCA) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base C (52/50); new base T (28/34); total (80/84) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01 | |||||||||||
Only 100 of 172 total aligned reads displayed.
GAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTG > Exported/1933075‑1933213 | gAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCcg > 1:4037584/1‑71 (MQ=255) aaCATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCcgc < 1:2846754/71‑1 (MQ=255) cATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGt < 1:4430440/71‑1 (MQ=255) cATGGCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTACTGGAAACGTTCAGCCTGATCTTCTGCGt > 1:2382617/1‑71 (MQ=255) tggCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTcg < 1:304919/69‑2 (MQ=255) tggCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTcg < 1:4602582/69‑2 (MQ=255) tCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCa < 1:3810493/71‑1 (MQ=255) ggCTTCGTCGTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTcg < 1:567082/69‑2 (MQ=255) gCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCAtt > 1:5288223/1‑71 (MQ=255) cTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTc < 1:2975721/71‑1 (MQ=255) tcgtcgTCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGttt < 1:102543/69‑2 (MQ=255) cgtcgtCACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGtttaa < 1:185405/70‑4 (MQ=255) gtcgtcACCTGCAGCTTTAGCATTAACCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGtttaa > 1:2656606/1‑66 (MQ=255) tcgtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTc > 1:2887221/1‑71 (MQ=255) gtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGc > 1:5584112/1‑71 (MQ=255) cTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAg > 1:2133253/1‑71 (MQ=255) cGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGcc < 1:3043267/70‑1 (MQ=255) cGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCg > 1:3763480/1‑71 (MQ=255) tCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGt > 1:5461608/1‑70 (MQ=255) cTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTa > 1:3019898/1‑71 (MQ=255) cTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTa > 1:2278565/1‑71 (MQ=255) ttCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGc > 1:4955044/1‑64 (MQ=255) ttCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGt > 1:1723802/1‑67 (MQ=255) gcgGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCg > 1:3878992/1‑54 (MQ=255) ggCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt < 1:4699661/71‑1 (MQ=255) ggCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt < 1:234870/71‑1 (MQ=255) ttaaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTc < 1:1364007/69‑1 (MQ=255) gCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt < 1:5256005/70‑1 (MQ=255) gCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTc < 1:1266388/71‑1 (MQ=255) taaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTaaa < 1:1425217/54‑1 (MQ=255) cAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCa > 1:5270196/1‑71 (MQ=255) aaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCAc > 1:701376/1‑71 (MQ=255) aaCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAg > 1:182549/1‑45 (MQ=255) aaCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAc < 1:2697084/62‑1 (MQ=255) aaCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAc < 1:1558429/62‑1 (MQ=255) aaCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCAc < 1:333592/71‑1 (MQ=255) aaCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCAc < 1:5395818/71‑1 (MQ=255) aCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCa > 1:3909972/1‑69 (MQ=255) aCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTc < 1:3042499/38‑1 (MQ=255) aCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCAtt > 1:3491150/1‑41 (MQ=255) ccTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTc < 1:1654214/37‑1 (MQ=255) ccTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGt > 1:2076823/1‑57 (MQ=255) cTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGac < 1:1620121/71‑1 (MQ=255) cTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTc > 1:4158752/1‑40 (MQ=255) cTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGac < 1:5101936/71‑1 (MQ=255) gttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGacca > 1:3179684/3‑71 (MQ=255) gttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGacca > 1:571443/3‑71 (MQ=255) gttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGacca > 1:2647253/3‑71 (MQ=255) gATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCt < 1:2515325/42‑1 (MQ=255) gATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCt < 1:1987914/42‑1 (MQ=255) gATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCt < 1:5614661/42‑1 (MQ=255) gATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCt < 1:5621368/42‑1 (MQ=255) gATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAg < 1:545302/50‑1 (MQ=255) gATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGacca > 1:3731627/1‑71 (MQ=255) ttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccac < 1:602000/70‑1 (MQ=255) ttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccac < 1:394704/70‑1 (MQ=255) ttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGacc < 1:3514483/68‑1 (MQ=255) tCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGaccac > 1:2465566/1‑70 (MQ=255) tCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGaccac > 1:4423919/1‑70 (MQ=255) ccAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAt > 1:4631645/1‑58 (MQ=255) ccAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAt > 1:2960401/1‑58 (MQ=255) aGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAAt < 1:4794449/71‑1 (MQ=255) ggAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAAt > 1:406378/1‑70 (MQ=255) gAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGaccacc < 1:4235684/66‑1 (MQ=255) gAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTa > 1:328915/1‑49 (MQ=255) gAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATg < 1:2241059/70‑1 (MQ=255) aaGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt > 1:4831013/1‑55 (MQ=255) aGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGa > 1:2620785/1‑59 (MQ=255) aGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATgaag < 1:391826/71‑1 (MQ=255) aGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATgaag < 1:5458297/71‑1 (MQ=255) gCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt < 1:33644/53‑1 (MQ=255) gcgcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCAc > 1:2181999/1‑51 (MQ=255) gcgcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCAc > 1:3602863/1‑51 (MQ=255) gcgcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCaa > 1:1466576/1‑60 (MQ=255) gcgcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTc > 1:5641659/1‑70 (MQ=255) gcgcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCAGTTACCGATTTCACGa > 1:3826639/1‑53 (MQ=255) cagcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccacc > 1:1881319/3‑58 (MQ=255) cagcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccacc > 1:1140282/3‑58 (MQ=255) cagcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATga > 1:4404693/3‑63 (MQ=255) cagcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTc > 1:1032166/3‑70 (MQ=255) cagcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTc > 1:4696600/3‑70 (MQ=255) agcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCaa < 1:4362054/70‑1 (MQ=255) gcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCa > 1:1285278/1‑69 (MQ=255) ccTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATga < 1:4215128/60‑1 (MQ=255) ccTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATga < 1:2214339/60‑1 (MQ=255) cTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGc > 1:1817262/1‑71 (MQ=255) tGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccacc < 1:353530/53‑1 (MQ=255) tGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAcc < 1:2022305/38‑1 (MQ=255) tGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt > 1:5161285/1‑43 (MQ=255) tGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCg > 1:3995602/1‑71 (MQ=255) aTCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccaccga > 1:5696095/1‑51 (MQ=255) cTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGac > 1:21220/1‑45 (MQ=255) cTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTc > 1:162298/1‑70 (MQ=255) cTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCa > 1:2730274/1‑41 (MQ=255) cTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCa > 1:3134762/1‑41 (MQ=255) ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTc < 1:4732972/67‑1 (MQ=255) ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCtgt < 1:148358/70‑1 (MQ=255) ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCt < 1:3784642/68‑1 (MQ=255) ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCt < 1:181014/68‑1 (MQ=255) cgcgTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCtgtg < 1:5053157/70‑1 (MQ=255) | GAACATCGCTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTG > Exported/1933075‑1933213 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |