| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,933,153 | C→T | 31.1% | L430L (CTG→CTA) ‡ | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,933,153 | 0 | C | T | 31.1% | 191.5 / 113.4 | 148 | L430L (CTG→CTA) ‡ | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base C (49/53); new base T (23/23); total (72/76) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-01 | |||||||||||
Only 100 of 152 total aligned reads displayed.
CTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGG > Exported/1933083‑1933222 | cTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTc < 1:2975721/71‑1 (MQ=255) tcgtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTc < 1:5644894/71‑1 (MQ=255) tcgtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTc > 1:2887221/1‑71 (MQ=255) gtcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGc > 1:5584112/1‑71 (MQ=255) tcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCt < 1:1432638/71‑1 (MQ=255) tcACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCt < 1:5443102/71‑1 (MQ=255) cTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAg > 1:2133253/1‑71 (MQ=255) cTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTa > 1:2278565/1‑71 (MQ=255) cTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTa > 1:3019898/1‑71 (MQ=255) tttCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAc > 1:1110760/1‑71 (MQ=255) ttCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGc > 1:4955044/1‑64 (MQ=255) ttCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGt > 1:1723802/1‑67 (MQ=255) tCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAcc < 1:3059724/70‑1 (MQ=255) tCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAc < 1:3032305/69‑1 (MQ=255) gcgGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCg > 1:4927850/1‑54 (MQ=255) ggCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt < 1:4699661/71‑1 (MQ=255) ggCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt < 1:234870/71‑1 (MQ=255) ttaaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCAtt < 1:3140496/60‑1 (MQ=255) ttaaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCAtt < 1:5163388/60‑1 (MQ=255) ttaaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGAttt > 1:4394814/3‑70 (MQ=255) ttaaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTc < 1:1364007/69‑1 (MQ=255) ttaaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTc < 1:2611400/69‑1 (MQ=255) gCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTc < 1:1266388/71‑1 (MQ=255) taaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTaaa < 1:1425217/54‑1 (MQ=255) taaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTaaa < 1:2479176/54‑1 (MQ=255) cAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCa > 1:5270196/1‑71 (MQ=255) aaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCAc > 1:701376/1‑71 (MQ=255) aaCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCAc < 1:5395818/71‑1 (MQ=255) aaCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCAc < 1:3734483/71‑1 (MQ=255) aCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCa > 1:3909972/1‑69 (MQ=255) ccTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGt > 1:2076823/1‑57 (MQ=255) ccTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAcc < 1:227153/61‑1 (MQ=255) cTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGac < 1:1620121/71‑1 (MQ=255) cTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTc > 1:2684931/1‑40 (MQ=255) cTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTc > 1:4158752/1‑40 (MQ=255) tGATCCAGGAAGCGTTGCGCTTGATCTTCCGCGTCATTc < 1:1818849/39‑1 (MQ=255) gttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGacca > 1:5457012/3‑71 (MQ=255) gttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGacca > 1:3179684/3‑71 (MQ=255) gATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCt < 1:5621368/42‑1 (MQ=255) gATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGacca > 1:3731627/1‑71 (MQ=255) ttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccac < 1:602000/70‑1 (MQ=255) ttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccac < 1:394704/70‑1 (MQ=255) ttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGacc < 1:3514483/68‑1 (MQ=255) aTCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAcc < 1:178907/57‑1 (MQ=255) tCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGaccac > 1:4423919/1‑70 (MQ=255) tCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGaccac > 1:2465566/1‑70 (MQ=255) ccAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAt > 1:2960401/1‑58 (MQ=255) aGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAAt < 1:4794449/71‑1 (MQ=255) ggAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAAt > 1:406378/1‑70 (MQ=255) gAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGaccacc < 1:4235684/66‑1 (MQ=255) gAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGAttt > 1:4172145/1‑56 (MQ=255) gAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATg < 1:2011664/70‑1 (MQ=255) aaGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGcc > 1:2189832/1‑44 (MQ=255) aaGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt > 1:2858329/1‑55 (MQ=255) aGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGa > 1:2620785/1‑59 (MQ=255) aGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATgaag < 1:5458297/71‑1 (MQ=255) aGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATgaag < 1:1850030/71‑1 (MQ=255) gCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt < 1:33644/53‑1 (MQ=255) gCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt < 1:3897859/53‑1 (MQ=255) gCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATgaag > 1:4574801/1‑70 (MQ=255) gTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTa > 1:3597298/1‑44 (MQ=255) gcgcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCAc > 1:3773649/1‑51 (MQ=255) gcgcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCAc > 1:4741048/1‑51 (MQ=255) gcgcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCAGTTACCGATTTCACGa > 1:3826639/1‑53 (MQ=255) cagcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccacc > 1:1881319/3‑58 (MQ=255) cagcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccacc > 1:1547396/3‑58 (MQ=255) cagcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTc > 1:1032166/3‑70 (MQ=255) agcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCaa < 1:4686757/70‑1 (MQ=255) agcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCaa < 1:3293834/70‑1 (MQ=255) agcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCaa < 1:4362054/70‑1 (MQ=255) agcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCa < 1:3045636/69‑1 (MQ=255) gcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCa > 1:1285278/1‑69 (MQ=255) ccTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATga < 1:2214339/60‑1 (MQ=255) cTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGc > 1:1817262/1‑71 (MQ=255) tGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccacc < 1:5623186/53‑1 (MQ=255) tGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccacc < 1:353530/53‑1 (MQ=255) tGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt > 1:5161285/1‑43 (MQ=255) tGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAt < 1:1982/41‑1 (MQ=255) tGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCg > 1:3995602/1‑71 (MQ=255) gATCTTCTGCGTCGTTTAATTCGCTAAAACCATTAACGATTTCACGACCACCGATGAAGAATTCAAAACgg > 1:579612/1‑71 (MQ=255) gATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCAc > 1:3852919/1‑45 (MQ=255) aTCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccaccga > 1:4704987/1‑51 (MQ=255) aTCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccaccga > 1:5696095/1‑51 (MQ=255) aTCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGt > 1:5260041/1‑71 (MQ=255) cTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGac > 1:21220/1‑45 (MQ=255) cTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGac > 1:5594233/1‑45 (MQ=255) cTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTc > 1:162298/1‑70 (MQ=255) cTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCa > 1:2730274/1‑41 (MQ=255) ttCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTcgg > 1:5093520/1‑69 (MQ=255) ttCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCaa > 1:2649409/1‑50 (MQ=255) ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCtgt < 1:148358/70‑1 (MQ=255) ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCt < 1:3784642/68‑1 (MQ=255) cgcgTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCtgtg < 1:5053157/70‑1 (MQ=255) tCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGAttt < 1:2735161/70‑1 (MQ=255) cATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTc < 1:1971917/61‑1 (MQ=255) aTTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAAt < 1:2417751/42‑1 (MQ=255) aTTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTcc < 1:1360530/70‑1 (MQ=255) ttCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCaa < 1:4072196/40‑1 (MQ=255) tCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCggg > 1:2018706/1‑71 (MQ=255) tCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCggg > 1:4314297/1‑71 (MQ=255) | CTTCGTCGTCACCTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGG > Exported/1933083‑1933222 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |