| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,933,165 | G→A | 35.3% | G426G (GGC→GGT) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,933,165 | 0 | G | A | 35.3% | 153.7 / 132.5 | 150 | G426G (GGC→GGT) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (51/46); new base A (27/26); total (78/72) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01 | |||||||||||
Only 100 of 159 total aligned reads displayed.
CTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTAC > Exported/1933095‑1933235 | cTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAg > 1:2133253/1‑71 (MQ=255) tCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGt > 1:5461608/1‑70 (MQ=255) cTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTa > 1:3019898/1‑71 (MQ=255) cTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTa > 1:2278565/1‑71 (MQ=255) tttCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAc > 1:1110760/1‑71 (MQ=255) ttCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGc > 1:4955044/1‑64 (MQ=255) ttCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGt > 1:1723802/1‑67 (MQ=255) tCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAcc < 1:3059724/70‑1 (MQ=255) tCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAc < 1:3032305/69‑1 (MQ=255) ggCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt < 1:4699661/71‑1 (MQ=255) ttaaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCAtt < 1:3140496/60‑1 (MQ=255) gCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt < 1:5256005/70‑1 (MQ=255) gCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTc < 1:1266388/71‑1 (MQ=255) aaCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCAc > 1:701376/1‑71 (MQ=255) aaCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTAc < 1:2697084/62‑1 (MQ=255) aaCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCAc < 1:333592/71‑1 (MQ=255) aaCCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCAc < 1:3734483/71‑1 (MQ=255) aCCTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCa > 1:3909972/1‑69 (MQ=255) ccTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGt > 1:2076823/1‑57 (MQ=255) cTGTTCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGac < 1:1620121/71‑1 (MQ=255) tGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGac > 1:1417998/1‑70 (MQ=255) gttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGacca > 1:5457012/3‑71 (MQ=255) gttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGacca > 1:2647253/3‑71 (MQ=255) gATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAg < 1:545302/50‑1 (MQ=255) gATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGacca > 1:3731627/1‑71 (MQ=255) ttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccac < 1:602000/70‑1 (MQ=255) ttCCTGGAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGacc < 1:3514483/68‑1 (MQ=255) tCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGaccac > 1:2465566/1‑70 (MQ=255) tCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGaccac > 1:4423919/1‑70 (MQ=255) ccAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAt > 1:4631645/1‑58 (MQ=255) aGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAAt < 1:4794449/71‑1 (MQ=255) aGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAAt < 1:43334/71‑1 (MQ=255) ggAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAAt > 1:406378/1‑70 (MQ=255) gAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGaccacc < 1:4235684/66‑1 (MQ=255) gAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGAttt > 1:4172145/1‑56 (MQ=255) gAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATga < 1:2335700/71‑1 (MQ=255) gAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATg < 1:2241059/70‑1 (MQ=255) gAAACGTTCAGCCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATg < 1:2011664/70‑1 (MQ=255) aaGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGcc > 1:2189832/1‑44 (MQ=255) aaGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt > 1:2858329/1‑55 (MQ=255) aGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGa > 1:2620785/1‑59 (MQ=255) aGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATgaag < 1:391826/71‑1 (MQ=255) gCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATgaag > 1:4574801/1‑70 (MQ=255) ttGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACt < 1:5328050/71‑1 (MQ=255) gcgcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCAc > 1:3602863/1‑51 (MQ=255) gcgcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTc > 1:5641659/1‑70 (MQ=255) gcgcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCAGTTACCGATTTCACGa > 1:3826639/1‑53 (MQ=255) cagcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccacc > 1:1547396/3‑58 (MQ=255) cagcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccacc > 1:1881319/3‑58 (MQ=255) cagcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATga > 1:4404693/3‑63 (MQ=255) cagcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTc > 1:1032166/3‑70 (MQ=255) cagcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTc > 1:4696600/3‑70 (MQ=255) agcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCaa < 1:4362054/70‑1 (MQ=255) agcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCaa < 1:3293834/70‑1 (MQ=255) agcCTGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCa < 1:3045636/69‑1 (MQ=255) gcCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCa > 1:300300/1‑69 (MQ=255) ccTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATga < 1:2214339/60‑1 (MQ=255) ccTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATg < 1:1356268/59‑1 (MQ=255) cTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGc > 1:1817262/1‑71 (MQ=255) tGATCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccacc < 1:353530/53‑1 (MQ=255) tGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAttt > 1:5161285/1‑43 (MQ=255) tGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAt < 1:1982/41‑1 (MQ=255) tGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGAt < 1:2914738/41‑1 (MQ=255) tGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCg > 1:3995602/1‑71 (MQ=255) gATCTTCTGCGTCGTTTAATTCGCTAAAACCATTAACGATTTCACGACCACCGATGAAGAATTCAAAACgg > 1:579612/1‑71 (MQ=255) gATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCAc > 1:3852919/1‑45 (MQ=255) aTCTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGaccaccga > 1:5696095/1‑51 (MQ=255) aTCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGt > 1:5260041/1‑71 (MQ=255) cTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGac > 1:21220/1‑45 (MQ=255) cTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGac > 1:5594233/1‑45 (MQ=255) cTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTc > 1:162298/1‑70 (MQ=255) ttCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTcgg > 1:5093520/1‑69 (MQ=255) ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGt < 1:3795329/66‑1 (MQ=255) ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTc < 1:4732972/67‑1 (MQ=255) ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCtgt < 1:148358/70‑1 (MQ=255) ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCt < 1:3784642/68‑1 (MQ=255) ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCt < 1:181014/68‑1 (MQ=255) cgcgTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCtgtg < 1:5053157/70‑1 (MQ=255) cgTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGAtt < 1:1958619/71‑1 (MQ=255) tCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGAttt < 1:2735161/70‑1 (MQ=255) aTTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTcc < 1:1360530/70‑1 (MQ=255) ttCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCaa < 1:4072196/40‑1 (MQ=255) tCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCggg > 1:2018706/1‑71 (MQ=255) attCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGtt < 1:4205209/68‑1 (MQ=255) ttCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTaa > 1:1234982/2‑71 (MQ=255) tCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAAc > 1:3613736/1‑71 (MQ=255) tCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCaa < 1:3771975/46‑1 (MQ=255) cGCTAAAACCATTACCGATTTCACGACCACCGATgaaga > 1:5382863/1‑39 (MQ=37) gCTAAAGCCGTTACCGATTTCACGACCACCAATga > 1:3170531/1‑35 (MQ=255) gCTAAAGCCGTTACCGATTTCACGACCACCAATga > 1:2763212/1‑35 (MQ=255) gCTAAAACCATTACCGATTTTACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAAcat < 1:4925232/71‑3 (MQ=255) gCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAAcat < 1:321381/71‑3 (MQ=255) gCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAAca < 1:5627787/70‑2 (MQ=255) aaaaCCATTACCGATTTCACGACCACCGATGAAGAATTc < 1:4824171/39‑1 (MQ=25) aaaaCCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAAcatcat > 1:2585388/1‑69 (MQ=255) aaaaCCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAAcatcat > 1:2156035/1‑69 (MQ=255) aaaGCCGTTACCGATTTCACGACCACCAATgaagaa > 1:4497982/1‑36 (MQ=255) aGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGc < 1:5349877/42‑1 (MQ=255) gCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTac > 1:3459425/1‑71 (MQ=255) gCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTac > 1:2357625/1‑71 (MQ=255) | CTGCGTCTTTCGCGGCAACCTGATCCAGGAAGCGTTGCGCCTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTAC > Exported/1933095‑1933235 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |