| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,933,276 | G→A | 38.0% | F389F (TTC→TTT) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,933,276 | 0 | G | A | 38.0% | 114.1 / 125.3 | 129 | F389F (TTC→TTT) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (35/45); new base A (30/19); total (65/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.98e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 135 total aligned reads displayed.
GTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACC > Exported/1933211‑1933344 | gtgATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGt > 1:4739304/1‑71 (MQ=255) tgATTTCCGGGTTAACGTCGTTACGACGCGCAAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTc > 1:4597413/1‑71 (MQ=255) tgATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGtt < 1:310383/71‑2 (MQ=255) cGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGaa > 1:239370/1‑71 (MQ=255) gTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAAt > 1:5534655/1‑69 (MQ=255) gTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCa < 1:5486266/71‑1 (MQ=255) ttAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAg < 1:5676097/71‑1 (MQ=255) ttAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAg < 1:2243293/71‑1 (MQ=255) aaCGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGa < 1:1599594/70‑1 (MQ=255) aaCATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGaa > 1:5617751/1‑65 (MQ=255) aaCATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGaa > 1:1530850/1‑65 (MQ=255) aaCATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGAt > 1:4055516/1‑71 (MQ=255) cgtcgtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATg < 1:2597515/50‑1 (MQ=255) cgtcgtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATg < 1:3767432/50‑1 (MQ=255) tcattACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGa > 1:5360930/4‑66 (MQ=255) tcattACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGAtg < 1:2922824/65‑1 (MQ=255) gtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCtt > 1:2449454/1‑71 (MQ=255) gtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCtt < 1:3358170/71‑1 (MQ=255) attACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAg > 1:580606/2‑49 (MQ=19) attACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAg > 1:4785117/2‑49 (MQ=19) attACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGtt > 1:2834892/2‑51 (MQ=19) attACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGtt > 1:4030066/2‑51 (MQ=19) ttACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATaaa < 1:2350342/47‑1 (MQ=19) ttACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGttg < 1:3570228/52‑3 (MQ=19) ttACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTc > 1:2088010/1‑71 (MQ=255) tACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCa < 1:40475/60‑1 (MQ=255) gacgCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGcc < 1:3442001/71‑1 (MQ=255) cgcgCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCAc < 1:2829183/71‑1 (MQ=255) gcgcCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAAt < 1:5121648/52‑1 (MQ=255) ccAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCActtca > 1:5224330/1‑70 (MQ=255) ccAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCActtct > 1:243279/1‑71 (MQ=255) cAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCtt < 1:366448/59‑1 (MQ=255) gCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCActtcata > 1:4625207/1‑67 (MQ=255) gCGGAGAAACTTCTGCCGGATATTCAGTAATGAAgg > 1:2318334/1‑36 (MQ=255) gCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCg < 1:934750/71‑1 (MQ=255) cGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGcc < 1:336913/61‑1 (MQ=255) gagaAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCAc > 1:2806325/1‑61 (MQ=255) gagaAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCAc > 1:3008522/1‑61 (MQ=255) gaAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGc < 1:1816081/50‑1 (MQ=255) gaAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAt < 1:1459244/71‑1 (MQ=255) gaAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAt < 1:1602002/71‑1 (MQ=255) aaaCTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAtc < 1:3332629/71‑1 (MQ=255) caCTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGa > 1:4206593/2‑43 (MQ=25) aaCTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAtct > 1:4452494/1‑71 (MQ=255) aCTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGaa > 1:686656/1‑37 (MQ=25) aCTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAtctc > 1:3067115/1‑71 (MQ=255) cTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGc < 1:2199425/46‑1 (MQ=37) cTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAg < 1:125500/65‑1 (MQ=255) ttCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGc < 1:4661222/45‑1 (MQ=38) ttCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCaaa > 1:4159003/1‑63 (MQ=255) ttCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGAtctctg > 1:1675878/1‑69 (MQ=255) ttCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGa > 1:2596696/1‑40 (MQ=255) ttCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGAt > 1:3007052/1‑41 (MQ=255) ttCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCgg > 1:659157/1‑71 (MQ=255) ttCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGAGCTTCTGCCACTTCTTCGAAGATCTCgg > 1:2016147/1‑71 (MQ=255) tCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCtt > 1:1080521/1‑46 (MQ=255) tCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAt > 1:3369534/1‑65 (MQ=255) tCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAt > 1:2316289/1‑65 (MQ=255) cTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGAt > 1:2297552/1‑39 (MQ=37) cTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGAt > 1:4871786/1‑39 (MQ=37) cTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGAt > 1:1123035/1‑39 (MQ=37) cTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGt > 1:1264060/1‑70 (MQ=255) ccGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAAc < 1:5257695/70‑1 (MQ=255) ccGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAAc < 1:2198002/70‑1 (MQ=255) ccGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAAc < 1:4175604/70‑1 (MQ=255) ccGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAAc < 1:3331715/70‑1 (MQ=255) ccGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAAc < 1:5251145/70‑1 (MQ=255) ccGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACg < 1:2950561/71‑1 (MQ=255) cGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGAcaa < 1:3572823/71‑3 (MQ=255) cGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGAcaa < 1:3931061/71‑3 (MQ=255) cGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGAcaa < 1:1224416/71‑3 (MQ=255) cGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa < 1:688790/71‑1 (MQ=255) ggATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAAt < 1:2151697/71‑1 (MQ=255) ggATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGAt < 1:4125749/71‑1 (MQ=255) atatTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATAc < 1:1503976/71‑1 (MQ=255) atatTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATAc > 1:5397678/1‑71 (MQ=255) tCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGa < 1:2718328/49‑1 (MQ=255) tCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGa < 1:5684663/49‑1 (MQ=255) tCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGcc > 1:3587386/1‑71 (MQ=255) tCAGTAATGAAGGTCGGATGAATCAGATGTGCTTCTGCCACTTCTTCGa < 1:3886211/49‑1 (MQ=255) ccgTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTcc > 1:880591/3‑70 (MQ=255) ccgTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTcc > 1:228746/3‑70 (MQ=255) cAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCg > 1:4408036/1‑56 (MQ=255) cAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGcc > 1:1606530/1‑70 (MQ=255) cgTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTccc < 1:5349380/69‑1 (MQ=255) cgTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTcc > 1:4073074/2‑69 (MQ=255) aGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCa < 1:3773351/71‑1 (MQ=255) taataaAGGTTGGCTGAATCAGATGTGCTTCTGCCACttcatca < 1:2489814/44‑2 (MQ=37) taataaAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCaac < 1:2622100/71‑3 (MQ=255) aaTGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAg < 1:3744015/46‑1 (MQ=255) aaTGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGa < 1:3029277/70‑1 (MQ=255) aaTGAAGCTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAc > 1:3738040/1‑71 (MQ=255) aTGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcc > 1:5092796/1‑71 (MQ=255) aTGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcc > 1:2666226/1‑71 (MQ=255) aTGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcc > 1:3130815/1‑71 (MQ=255) tGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAtc < 1:4082040/47‑1 (MQ=255) tGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa < 1:171847/57‑1 (MQ=255) tGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa < 1:4639703/57‑1 (MQ=255) tGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa < 1:3930688/57‑1 (MQ=255) tGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa < 1:1088435/57‑1 (MQ=255) | GTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACC > Exported/1933211‑1933344 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |