| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,933,315 | G→A | 26.9% | F376F (TTC→TTT) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,933,315 | 0 | G | A | 26.9% | 279.9 / 110.2 | 171 | F376F (TTC→TTT) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (64/61); new base A (20/26); total (84/87) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-02 | |||||||||||
Only 100 of 176 total aligned reads displayed.
GCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAAT > Exported/1933245‑1933383 | gCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCg < 1:4607680/71‑1 (MQ=255) aaCTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAtct > 1:4452494/1‑71 (MQ=255) cTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAg < 1:125500/65‑1 (MQ=255) cTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAg < 1:157479/65‑1 (MQ=255) ttCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGAtctctg > 1:1675878/1‑69 (MQ=255) ttCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCgg > 1:659157/1‑71 (MQ=255) tCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAt > 1:2316289/1‑65 (MQ=255) tCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAt > 1:4072003/1‑65 (MQ=255) tCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAt > 1:3369534/1‑65 (MQ=255) cTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGt < 1:5263079/70‑1 (MQ=255) cTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGt > 1:1264060/1‑70 (MQ=255) ccGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAAc < 1:2198002/70‑1 (MQ=255) ccGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAAc < 1:3331715/70‑1 (MQ=255) ccGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAAc < 1:5251145/70‑1 (MQ=255) cGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa < 1:688790/71‑1 (MQ=255) ggATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGAt < 1:4125749/71‑1 (MQ=255) ttCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGAt < 1:3361860/66‑1 (MQ=255) tCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGcc > 1:3587386/1‑71 (MQ=255) ccgTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTcc > 1:228746/3‑70 (MQ=255) ccgTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTcc > 1:880591/3‑70 (MQ=255) cAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGcc > 1:1606530/1‑70 (MQ=255) cgTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTccc < 1:5349380/69‑1 (MQ=255) aaTGAAGCTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAc > 1:3738040/1‑71 (MQ=255) aTGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcc > 1:5092796/1‑71 (MQ=255) aTGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcc > 1:3130815/1‑71 (MQ=255) aTGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcc > 1:2666226/1‑71 (MQ=255) tGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAtc < 1:4082040/47‑1 (MQ=255) tGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAtc < 1:3058260/47‑1 (MQ=255) tGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa < 1:3930688/57‑1 (MQ=255) tGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa < 1:171847/57‑1 (MQ=255) aaGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGAtctct > 1:1996093/1‑47 (MQ=37) aaGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGAtctct > 1:3028351/1‑47 (MQ=37) aaGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATa > 1:450541/1‑57 (MQ=255) aaGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCa > 1:25551/1‑64 (MQ=255) aaGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAccc > 1:1942019/1‑69 (MQ=255) aGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAg < 1:375750/71‑1 (MQ=255) tCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa > 1:3669043/1‑51 (MQ=255) ggCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAAc > 1:488872/1‑47 (MQ=255) ggCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAc < 1:58183/61‑1 (MQ=255) ggCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAc < 1:2444210/61‑1 (MQ=255) ggCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAc < 1:545496/61‑1 (MQ=255) ggCTGAATCAGATGTGCTTCTGCCACTTCATCaaa < 1:2522397/35‑1 (MQ=37) ggCTGAATCAGATGTGCTTCTGCCACTTCATCaaa < 1:2374339/35‑1 (MQ=37) ggCTGAATCAGATGTGCTTCTGCCACTTCATCAAAg < 1:5643474/36‑1 (MQ=37) ggCTGAATCAGATGTGCTTCTGCCACTTCATCAAAg < 1:4648491/36‑1 (MQ=37) ggCTGAATCAGATGTGCTTCTGCCACTTCATCAAAg < 1:1167873/36‑1 (MQ=37) ggCTGAATCAGATGTGCTTCTGCCACTTCATCAAAg < 1:2047972/36‑1 (MQ=37) ggCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCa > 1:1904783/1‑65 (MQ=255) ggCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCa > 1:3610316/1‑65 (MQ=255) gCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa < 1:3830996/48‑1 (MQ=255) tGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAAt < 1:3573310/47‑1 (MQ=25) gAATCAGATGTGCTTCTGCCACTTCTTCGAATATCTCGGTAAAGa < 1:3153150/45‑1 (MQ=38) gAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGtga > 1:139788/1‑40 (MQ=25) gAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGtg > 1:2323336/1‑40 (MQ=25) aaTCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcccc > 1:1300250/1‑59 (MQ=255) aaTCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTtctc < 1:3421519/69‑1 (MQ=255) aaTCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCa < 1:3710205/70‑1 (MQ=255) aaTCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGCTTTtctcta > 1:1322916/1‑69 (MQ=255) aaTCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGCTTTtctc < 1:2721236/69‑1 (MQ=255) aaTCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGCTTTtctc < 1:1493536/69‑1 (MQ=255) aaTCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGCTTTtctc < 1:1853951/69‑1 (MQ=255) aTCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcc < 1:3894561/56‑1 (MQ=255) aTCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTtctc < 1:4173209/68‑1 (MQ=255) aTCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGAcaa > 1:5674402/1‑41 (MQ=21) tCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa < 1:2143698/42‑1 (MQ=255) tCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa < 1:215251/42‑1 (MQ=255) tCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcccc > 1:4681172/1‑57 (MQ=255) tCAGATGTGCTTCTGCCACTTCATCAGAGATCTCTGTGAcaa < 1:5548482/42‑3 (MQ=16) tCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGAcaa < 1:4724694/42‑3 (MQ=21) tCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGCTTTTCTCTAcc > 1:4148008/1‑70 (MQ=255) cAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa < 1:2003497/41‑1 (MQ=255) cAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCaa > 1:1590314/1‑68 (MQ=255) cAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGCTTTtctc > 1:135385/1‑66 (MQ=255) cAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGCTTTtctc > 1:50082/1‑66 (MQ=255) aGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGt < 1:2902844/70‑1 (MQ=255) aGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGCTTTTCTCTAccgt < 1:3852109/71‑4 (MQ=255) gATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTgg < 1:4687913/71‑1 (MQ=255) tgtgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGAt < 1:5369291/71‑1 (MQ=255) tgtgCTTCTGCCACTTCATCAAAGATCTCTGTAACAATACGTCCCa < 1:4562239/46‑1 (MQ=25) gtgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCa > 1:1040531/1‑45 (MQ=255) gtgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCtcttc > 1:4011272/1‑59 (MQ=255) tgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATg > 1:1089477/1‑70 (MQ=255) gCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCa < 1:3461882/60‑1 (MQ=255) cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCtcttc > 1:4239722/1‑56 (MQ=255) cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGcc > 1:3481753/1‑70 (MQ=255) cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGcc > 1:1536290/1‑70 (MQ=255) cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGcc > 1:1034932/1‑70 (MQ=255) tCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCa > 1:3955280/1‑40 (MQ=255) tCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCa > 1:2966395/1‑40 (MQ=255) cTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGa < 1:2313055/69‑1 (MQ=255) gCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTtctc > 1:3801942/1‑53 (MQ=255) cttcttCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGAtt > 1:548779/1‑69 (MQ=255) cttcttCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGAtt > 1:5553026/1‑69 (MQ=255) tcttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAg > 1:2793419/1‑70 (MQ=255) tcttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAg > 1:296842/1‑70 (MQ=255) cttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGc < 1:697422/70‑1 (MQ=255) ttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGAt < 1:2489382/54‑1 (MQ=255) tcGAATATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAAt > 1:2191541/1‑71 (MQ=255) tcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGAt < 1:2928213/63‑1 (MQ=255) cGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAg > 1:3270839/1‑66 (MQ=255) | GCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAAT > Exported/1933245‑1933383 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |