| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,933,591 | G→A | 22.8% | Y284Y (TAC→TAT) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,933,591 | 0 | G | A | 22.8% | 212.5 / 57.9 | 114 | Y284Y (TAC→TAT) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (48/40); new base A (13/13); total (61/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.96e-01 | |||||||||||
Only 100 of 114 total aligned reads displayed.
AGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAG > Exported/1933526‑1933660 | agaATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGc > 1:251775/1‑71 (MQ=255) aaTATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCa < 1:3865103/71‑1 (MQ=255) aaTATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCa > 1:5144597/1‑71 (MQ=255) atCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATg > 1:4320873/1‑70 (MQ=255) tCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATg > 1:3359077/1‑69 (MQ=255) tCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATg > 1:2809981/1‑69 (MQ=255) tCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTa < 1:4120994/71‑1 (MQ=255) cTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTag < 1:1164551/70‑1 (MQ=255) cTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTag < 1:2918867/70‑1 (MQ=255) cTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTa > 1:2636665/1‑69 (MQ=255) tgtgCCAGGGTGCGGAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTagag > 1:489102/1‑71 (MQ=255) gtgCCAGGGTGCGGAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGt > 1:4180999/1‑71 (MQ=255) gtgCCAGGGTGCGGAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGt > 1:2505897/1‑71 (MQ=255) gtgCCAGGGTGCGGAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGt > 1:2978081/1‑71 (MQ=255) ccAGGGTGCGGAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTc < 1:2002871/70‑1 (MQ=255) agagTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCa > 1:4795297/1‑58 (MQ=255) agagTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCa > 1:4668741/1‑58 (MQ=255) agagTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcat < 1:4582410/71‑1 (MQ=255) agagTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcat < 1:4214173/71‑1 (MQ=255) gagTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcat < 1:3095157/70‑1 (MQ=255) agTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcatc > 1:1944328/1‑70 (MQ=255) agTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCAGCGTa > 1:2250359/1‑51 (MQ=255) tACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATg < 1:3973897/71‑1 (MQ=255) cGGAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGt < 1:5607190/71‑1 (MQ=255) cGGAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGt < 1:4610910/71‑1 (MQ=255) cGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGt > 1:3218272/1‑71 (MQ=255) cGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGt > 1:3125452/1‑71 (MQ=255) cGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGt > 1:3174490/1‑71 (MQ=255) ggAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATtgtg < 1:1453187/71‑1 (MQ=255) ggAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATtgtg < 1:2298891/71‑1 (MQ=255) ggAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTa < 1:4345748/55‑1 (MQ=255) ggAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTg < 1:1628587/71‑1 (MQ=255) gAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATtgtg < 1:3540865/70‑1 (MQ=255) gAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTg > 1:4119688/1‑70 (MQ=255) aaCAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGaa > 1:4333181/1‑71 (MQ=255) aaCAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGaa < 1:655701/71‑1 (MQ=255) aCAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGt < 1:4098631/67‑1 (MQ=255) cAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAAct < 1:4462152/71‑1 (MQ=255) cAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAAct > 1:573568/1‑71 (MQ=255) aGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAActc > 1:3809577/1‑71 (MQ=255) gCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAActct > 1:3805927/1‑71 (MQ=255) gCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAActct > 1:4744690/1‑71 (MQ=255) gCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAActct > 1:2719605/1‑71 (MQ=255) gCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAActc > 1:4926756/1‑70 (MQ=255) cGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTg > 1:4254158/1‑71 (MQ=255) aTTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTagag > 1:4052027/1‑49 (MQ=255) aTTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTagag > 1:2863214/1‑49 (MQ=255) aTTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTagag > 1:2781024/1‑49 (MQ=255) aTTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTag < 1:1526961/47‑1 (MQ=255) aTTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTgg > 1:1752748/1‑70 (MQ=255) cGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAAct < 1:2997913/63‑1 (MQ=255) cGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTa > 1:524734/1‑71 (MQ=255) tgTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTAt > 1:4743265/2‑71 (MQ=255) gTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATg < 1:658033/71‑1 (MQ=255) tCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTc > 1:1794025/1‑46 (MQ=255) tCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCcatcatt < 1:4831082/53‑2 (MQ=255) tCAGTTCAATCAAATAGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTa < 1:3702415/68‑1 (MQ=255) tCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGtt < 1:4874424/67‑1 (MQ=255) aGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACg > 1:2144389/1‑71 (MQ=255) aGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACg > 1:1631772/1‑71 (MQ=255) aGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACg > 1:1337695/1‑71 (MQ=255) cGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAActct < 1:290926/56‑1 (MQ=255) cGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAActct < 1:249314/56‑1 (MQ=255) cGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAActct < 1:3499983/56‑1 (MQ=255) cGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACg > 1:5580556/1‑71 (MQ=255) gATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTAc < 1:2618209/69‑1 (MQ=255) gATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTAc < 1:4813837/69‑1 (MQ=255) aTCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATgg < 1:3918940/46‑1 (MQ=255) aTCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATgg < 1:3696514/46‑1 (MQ=255) taaGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGa < 1:2749264/68‑1 (MQ=255) tCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCcatcat < 1:3926212/43‑1 (MQ=255) tCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGa > 1:5617105/1‑70 (MQ=255) aTCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcat < 1:4623723/36‑1 (MQ=255) aTCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcat < 1:5137049/36‑1 (MQ=255) aTCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGaa < 1:1727524/45‑1 (MQ=255) cTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACg > 1:3107546/1‑62 (MQ=255) tttGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACg < 1:5237807/57‑1 (MQ=255) tttGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACg < 1:1190534/57‑1 (MQ=255) gtggTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCtt > 1:5217119/4‑71 (MQ=255) gtggTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCtt > 1:2696559/4‑71 (MQ=255) gtggTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCtt > 1:4159908/4‑71 (MQ=255) ttGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTa > 1:110065/1‑51 (MQ=255) tGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTg < 1:70909/38‑1 (MQ=255) tGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTg < 1:1784391/38‑1 (MQ=255) tGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTa > 1:4402170/1‑57 (MQ=255) tGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCtt < 1:2841/69‑1 (MQ=255) tGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCt < 1:4508666/68‑1 (MQ=255) tGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCg > 1:4408375/1‑71 (MQ=255) gTAATCTGCGTAAGCCATGTAGAGTTCCATCATgg > 1:1801738/1‑35 (MQ=255) tAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTa > 1:3183721/1‑48 (MQ=255) tAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACg > 1:3072758/1‑53 (MQ=255) tAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGt < 1:3745819/70‑1 (MQ=255) tAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTca < 1:4238562/69‑2 (MQ=255) tAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTca < 1:2503967/69‑2 (MQ=255) aTCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGaaa > 1:3950377/1‑59 (MQ=255) aTCCGCATACGCCATGTAGAGTTACATCATTGTGAACTCAGGATTATGGCGAACAGaa > 1:4041847/1‑58 (MQ=255) tGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACgg > 1:3010245/1‑70 (MQ=255) tGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACgg > 1:46491/1‑70 (MQ=255) cGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGa < 1:2944428/69‑1 (MQ=255) cGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAg < 1:5230058/71‑1 (MQ=255) | AGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAG > Exported/1933526‑1933660 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |