| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,933,627 | G→A | 27.4% | N272N (AAC→AAT) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,933,627 | 0 | G | A | 27.4% | 273.3 / 110.3 | 168 | N272N (AAC→AAT) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (58/64); new base A (18/28); total (76/92) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 176 total aligned reads displayed.
CGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAA > Exported/1933560‑1933697 | cGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTa > 1:524734/1‑71 (MQ=255)cGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTa > 1:724880/1‑71 (MQ=255) gTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATg < 1:658033/71‑1 (MQ=255) tCAGTTCAATCAAATAGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTa < 1:3702415/68‑1 (MQ=255) tCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGtt < 1:4874424/67‑1 (MQ=255) aGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACg > 1:2144389/1‑71 (MQ=255) cGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTggg > 1:4682924/1‑59 (MQ=255) cGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCAATCATGGTGAACTCTggg > 1:2521287/1‑59 (MQ=255) taaGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGa < 1:2749264/68‑1 (MQ=255) tCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGa > 1:5617105/1‑70 (MQ=255) tttGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACg < 1:1190534/57‑1 (MQ=255) gtggTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCtt > 1:5217119/4‑71 (MQ=255) ttGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTa > 1:320873/1‑51 (MQ=255) tGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTa > 1:4402170/1‑57 (MQ=255) tGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCt < 1:4508666/68‑1 (MQ=255) tGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTc < 1:281655/70‑1 (MQ=255) tAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTa > 1:3183721/1‑48 (MQ=255) tAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACg > 1:3072758/1‑53 (MQ=255) tAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTca < 1:4238562/69‑2 (MQ=255) aTCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGaaa > 1:3950377/1‑59 (MQ=255) aTCCGCATACGCCATGTAGAGTTACATCATTGTGAACTCAGGATTATGGCGAACAGaa > 1:4041847/1‑58 (MQ=255) tGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACgg > 1:3010245/1‑70 (MQ=255) cGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAg < 1:5230058/71‑1 (MQ=255) aaGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTa > 1:2642069/1‑71 (MQ=255) cgCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTa < 1:1962743/69‑1 (MQ=255) cgCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTa < 1:2422989/69‑1 (MQ=255) aGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATa > 1:1555754/1‑52 (MQ=255) aGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTAc < 1:2409599/71‑1 (MQ=255) aGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTAc < 1:1531389/71‑1 (MQ=255) aGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTAc < 1:2954998/71‑1 (MQ=255) gCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACg < 1:1362355/71‑1 (MQ=255) aTGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGtt < 1:165428/71‑1 (MQ=255) aTGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGtt < 1:1890924/71‑1 (MQ=255) tGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGa < 1:1720756/60‑1 (MQ=255) gTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGaaa < 1:4776305/44‑1 (MQ=16) gTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGaaa < 1:2916993/44‑1 (MQ=16) gTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGa < 1:3789488/42‑1 (MQ=16) gTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGaaa > 1:5561247/1‑44 (MQ=255) gTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGaaa > 1:4270924/1‑44 (MQ=255) tAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGAt < 1:4017126/71‑1 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACg > 1:880187/1‑35 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGaaa > 1:4208479/1‑43 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa < 1:3602202/70‑1 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa < 1:1783731/70‑1 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAt > 1:3599301/1‑71 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAt > 1:5302943/1‑71 (MQ=255) agagTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGAt < 1:1984488/70‑1 (MQ=255) gagTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCtt > 1:3508873/1‑47 (MQ=255) gagTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCtt > 1:1417008/1‑47 (MQ=255) gagTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCt > 1:5110416/1‑46 (MQ=255) agTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTc > 1:565703/1‑71 (MQ=255) gTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCg < 1:3627925/71‑1 (MQ=255) gTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCg < 1:3840340/71‑1 (MQ=255) tCCATCATGGTGAACTATGGGTTATGACGTACGGAAATACCTTCGtt < 1:725456/47‑1 (MQ=255) ccATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGaa < 1:2076415/70‑1 (MQ=255) ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGaa > 1:4123391/1‑52 (MQ=255) ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGaa > 1:1003256/1‑52 (MQ=255) ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa > 1:3729672/1‑63 (MQ=255) ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa > 1:2682370/1‑63 (MQ=255) ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAttt > 1:2290000/1‑66 (MQ=255) ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAttt > 1:1314845/1‑66 (MQ=255) ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATAACTTCGTTACGGaa > 1:4739015/1‑52 (MQ=255) acatcatGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGaa > 1:2891802/2‑52 (MQ=255) catcatTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGtt < 1:1451763/60‑1 (MQ=255) catcatGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa < 1:5653391/62‑1 (MQ=255) catcatGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa < 1:5484193/62‑1 (MQ=255) catcatGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa < 1:5546947/62‑1 (MQ=255) tcatTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATacc > 1:1618597/1‑70 (MQ=255) tcatGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAt < 1:1116171/61‑1 (MQ=255) catGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATacac > 1:336618/1‑71 (MQ=255) atGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACg > 1:4177917/1‑71 (MQ=255) ggTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGa < 1:2793424/62‑1 (MQ=255) ggTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACg > 1:780594/1‑69 (MQ=255) gTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCt < 1:675221/70‑1 (MQ=255) gTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCt < 1:3970881/70‑1 (MQ=255) gTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCgtt > 1:1080564/1‑68 (MQ=255) tGAACTCAGGATTATGTCGAACAGAAATACCTTCATTACgg < 1:480592/41‑1 (MQ=255) tGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACgg < 1:1758720/41‑1 (MQ=16) tGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTca < 1:554714/71‑2 (MQ=255) gAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCg > 1:4582207/1‑70 (MQ=255) aaCTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGtt < 1:5358803/50‑1 (MQ=255) aaCTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGtt < 1:91075/50‑1 (MQ=255) aaCTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGtt < 1:1251588/50‑1 (MQ=255) aaCTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGaa < 1:4827354/71‑1 (MQ=255) aaCTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGaa < 1:4527674/71‑1 (MQ=255) aaCTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGaa < 1:430150/71‑1 (MQ=255) aaCTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTcaa < 1:2987779/70‑3 (MQ=255) ctctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGc > 1:4649937/1‑71 (MQ=255) ctctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGc < 1:4084731/71‑1 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACg < 1:5368982/42‑1 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGa < 1:4923416/65‑1 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGa < 1:4519719/65‑1 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGccac > 1:5437985/1‑71 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGccac > 1:4228905/1‑71 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGccac > 1:1408252/1‑71 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGc < 1:1602887/68‑1 (MQ=255) gggTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTACGAATACACGCTCGAAGccac > 1:2774212/1‑70 (MQ=255) ggTTATGACGTACGGAAATACCTTCGTTACGGAAGTTa > 1:1013563/1‑38 (MQ=255) ggTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAg > 1:2616005/1‑65 (MQ=255) ggTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGccacc < 1:4870123/70‑1 (MQ=255) | CGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAA > Exported/1933560‑1933697 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |