| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,934,155 | C→T | 26.8% | Q96Q (CAG→CAA) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,934,155 | 0 | C | T | 26.8% | 184.7 / 71.7 | 116 | Q96Q (CAG→CAA) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base C (40/44); new base T (29/2); total (70/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.30e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
Only 100 of 116 total aligned reads displayed.
GCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCAT > Exported/1934086‑1934223 | aGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAAt < 1:201982/71‑1 (MQ=255) gTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGc > 1:1882190/1‑71 (MQ=255) atCCCATTTTTTAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAAtac < 1:2274131/70‑3 (MQ=255) cccATTTTTTAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGaa > 1:506938/1‑66 (MQ=255) aTTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGAc > 1:296627/1‑69 (MQ=255) aTTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCg > 1:2943895/1‑71 (MQ=255) aTTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCg > 1:2809910/1‑71 (MQ=255) aTTTTTTAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTg > 1:3025360/1‑61 (MQ=255) aTTTTTTAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTg > 1:2434866/1‑61 (MQ=255) aTTTTTTAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTg > 1:4410789/1‑61 (MQ=255) aTTTTTTAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGcca > 1:3405972/1‑70 (MQ=255) aTTTTTTAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGcca > 1:2388906/1‑70 (MQ=255) tttttGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGc > 1:418436/1‑70 (MQ=255) tttttAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGCCAcc > 1:530027/1‑71 (MQ=255) ttttAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGaa > 1:4156094/1‑60 (MQ=255) ttttAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAAtac > 1:1574154/1‑61 (MQ=255) ttttAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAAta > 1:4884008/1‑61 (MQ=255) ttttAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAAta > 1:706701/1‑61 (MQ=255) ttttAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGcc > 1:4939613/1‑67 (MQ=255) ttttAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGCCAcc > 1:3243048/1‑70 (MQ=255) ttttAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGCCAcc > 1:3316314/1‑70 (MQ=255) tttGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCa < 1:2318093/70‑1 (MQ=255) taaaCTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGCCACCGAca > 1:3441751/3‑70 (MQ=255) taaaCTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGCCACCGAca > 1:2601234/3‑70 (MQ=255) gAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACg < 1:1604905/70‑1 (MQ=255) aaCTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTc < 1:2636232/71‑1 (MQ=255) aCTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGCCACCGACATcc > 1:3362005/1‑71 (MQ=255) cTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTg < 1:2561669/69‑1 (MQ=255) cTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCa < 1:3604473/71‑1 (MQ=255) cGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAgg > 1:4000042/1‑71 (MQ=255) gTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAggg > 1:3317548/1‑71 (MQ=255) gTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAggg < 1:1469185/71‑1 (MQ=255) tataAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGc < 1:4041612/65‑1 (MQ=255) tataAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGt > 1:218029/1‑70 (MQ=255) tataAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGt > 1:4648808/1‑70 (MQ=255) ataAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCt > 1:2273053/1‑41 (MQ=255) ataAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTaa > 1:3361187/1‑71 (MQ=255) ataAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTaa > 1:4515001/1‑71 (MQ=255) aaaCGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATg < 1:3328117/44‑1 (MQ=255) aaCGCCTTCCGGGAGATCGTCACGGGCAACGTCCAGCTGAATGCGACCGCCa > 1:1493144/1‑52 (MQ=255) cGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTc > 1:1558446/1‑55 (MQ=255) ttCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCg < 1:3309639/39‑1 (MQ=255) ttCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCg < 1:4397086/39‑1 (MQ=255) ttCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCg < 1:876855/39‑1 (MQ=255) ttCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGc < 1:3751635/55‑1 (MQ=255) tCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGa > 1:2600601/1‑39 (MQ=255) tCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGa > 1:1827217/1‑39 (MQ=255) tCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGc > 1:2419192/1‑71 (MQ=255) ccGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGAc > 1:30936/1‑39 (MQ=255) ccGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGAc > 1:4034452/1‑39 (MQ=255) ccGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGc < 1:315032/70‑1 (MQ=255) ccGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCt > 1:43532/1‑71 (MQ=255) ccGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCt > 1:830366/1‑71 (MQ=255) ccGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCt > 1:3594207/1‑71 (MQ=255) gggAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCttt < 1:2600185/71‑1 (MQ=255) ggAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTa < 1:4818060/71‑1 (MQ=255) gaTCGTCACGGGCAACGTACAGCTGAATGCGACCGcc > 1:3085563/1‑37 (MQ=255) tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:3923450/71‑1 (MQ=255) tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:1369846/71‑1 (MQ=255) tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataat < 1:4953092/71‑1 (MQ=255) tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:4254397/68‑1 (MQ=255) tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:1506555/68‑1 (MQ=255) tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:1500069/68‑1 (MQ=255) cACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAgg > 1:5028445/1‑44 (MQ=255) cACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAgg > 1:2967745/1‑44 (MQ=255) cACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGa > 1:3729867/1‑55 (MQ=255) aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac > 1:5635555/1‑71 (MQ=255) aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac > 1:4089260/1‑71 (MQ=255) aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac > 1:5192515/1‑71 (MQ=255) aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac > 1:2305793/1‑71 (MQ=255) aCGGGCAACGTACAGATGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataa > 1:2103692/1‑68 (MQ=255) cttgCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATac > 1:4010170/4‑70 (MQ=255) cGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa < 1:5206876/49‑1 (MQ=255) cGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa < 1:1083991/49‑1 (MQ=255) cGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa < 1:5608964/49‑1 (MQ=255) cGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa < 1:3553945/49‑1 (MQ=255) cGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac > 1:5431537/1‑70 (MQ=255) ttgCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATacga > 1:1882957/3‑71 (MQ=255) gCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTccc > 1:2435601/1‑59 (MQ=255) gCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTccc > 1:3486407/1‑59 (MQ=255) gCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATacga > 1:97317/1‑69 (MQ=255) cAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACgg > 1:1178934/1‑71 (MQ=255) aaCGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCa < 1:1086633/59‑1 (MQ=255) aCGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGt > 1:3189115/1‑71 (MQ=255) aCGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGt > 1:1821742/1‑71 (MQ=255) aCGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGt < 1:1442921/71‑1 (MQ=255) cGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGt > 1:2923742/1‑70 (MQ=255) gTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaaa < 1:4147612/43‑1 (MQ=255) gTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaaa < 1:2922051/43‑1 (MQ=255) gTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa < 1:5027833/41‑1 (MQ=255) gTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtca < 1:2699140/71‑1 (MQ=255) tACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGtcat < 1:459105/71‑1 (MQ=255) tACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACg < 1:3633276/46‑1 (MQ=255) tACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcat < 1:192176/71‑1 (MQ=255) tACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcat > 1:2695632/1‑71 (MQ=255) aCAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacg > 1:1351298/1‑61 (MQ=255) cAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaa > 1:256010/1‑39 (MQ=255) cAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatca > 1:2369524/1‑71 (MQ=255) cAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatc < 1:5568952/70‑1 (MQ=255) aGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTcatcat < 1:4756993/71‑1 (MQ=255) | GCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCAT > Exported/1934086‑1934223 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |