| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,934,200 | A→C | 28.9% | G81G (GGT→GGG) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,934,200 | 0 | A | C | 28.9% | 82.9 / 69.0 | 83 | G81G (GGT→GGG) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base A (33/26); new base C (13/11); total (46/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01 | |||||||||||
GGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCG > Exported/1934130‑1934269 | ggAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTa < 1:4818060/71‑1 (MQ=255) tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:3923450/71‑1 (MQ=255) tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:1369846/71‑1 (MQ=255) tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataat < 1:4953092/71‑1 (MQ=255) tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:4254397/68‑1 (MQ=255) tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:1506555/68‑1 (MQ=255) tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:1500069/68‑1 (MQ=255) aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac > 1:2305793/1‑71 (MQ=255) aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac > 1:3670634/1‑71 (MQ=255) aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac > 1:4089260/1‑71 (MQ=255) aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac > 1:5192515/1‑71 (MQ=255) aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac > 1:5635555/1‑71 (MQ=255) aCGGGCAACGTACAGATGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataa > 1:2103692/1‑68 (MQ=255) cttgCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATac > 1:4010170/4‑70 (MQ=255) cGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:3261681/65‑1 (MQ=255) cGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac > 1:5431537/1‑70 (MQ=255) ttgCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATacga > 1:1882957/3‑71 (MQ=255) gggCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacga < 1:4257391/71‑1 (MQ=255) gCAACGTACAGTTGAATCCGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTccc > 1:2586352/1‑59 (MQ=255) gCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTccc > 1:2435601/1‑59 (MQ=255) gCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTccc > 1:3486407/1‑59 (MQ=255) gCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATacga > 1:97317/1‑69 (MQ=255) cAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACgg > 1:1178934/1‑71 (MQ=255) aaCGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCa < 1:1086633/59‑1 (MQ=255) aCGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGt > 1:1821742/1‑71 (MQ=255) aCGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGt > 1:3189115/1‑71 (MQ=255) aCGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGt < 1:1442921/71‑1 (MQ=255) cGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGt > 1:2923742/1‑70 (MQ=255) gTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtca < 1:2699140/71‑1 (MQ=255) tACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGtcat < 1:459105/71‑1 (MQ=255) tACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcat < 1:192176/71‑1 (MQ=255) tACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcat > 1:2695632/1‑71 (MQ=255) aCAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacg > 1:1351298/1‑61 (MQ=255) cAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatca > 1:2369524/1‑71 (MQ=255) cAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatca > 1:2739834/1‑71 (MQ=255) cAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatc < 1:5568952/70‑1 (MQ=255) aGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTcatcat < 1:4756993/71‑1 (MQ=255) tGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCg > 1:65613/1‑71 (MQ=255) gAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCgg > 1:2526588/1‑71 (MQ=255) gAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCgg > 1:2287907/1‑71 (MQ=255) gAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCg > 1:1549295/1‑70 (MQ=255) gAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCg < 1:3245899/70‑1 (MQ=255) aaTGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCa < 1:3626458/47‑1 (MQ=255) aaTACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCgg < 1:4012404/70‑1 (MQ=255) aaTACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCg < 1:4738092/69‑1 (MQ=255) tGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataa < 1:5474961/48‑1 (MQ=255) tGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataa < 1:2251878/48‑1 (MQ=255) tGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacgacg > 1:3691850/1‑55 (MQ=255) tGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacgacg > 1:5491071/1‑55 (MQ=255) tGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacgac < 1:759894/54‑1 (MQ=255) tGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacgac < 1:63102/54‑1 (MQ=255) cGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAg > 1:1058439/1‑70 (MQ=255) cGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAg > 1:4926516/1‑70 (MQ=255) cGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAg > 1:3759086/1‑70 (MQ=255) gCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCaa > 1:932022/1‑68 (MQ=255) gCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCaa > 1:4279799/1‑68 (MQ=255) gCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCaa > 1:5591560/1‑68 (MQ=255) ccAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataa > 1:3067599/1‑40 (MQ=255) ccAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACgg > 1:1209557/1‑70 (MQ=255) ccAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGc < 1:587097/71‑1 (MQ=255) aaCGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGc < 1:3508765/63‑1 (MQ=255) cGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACg > 1:5157556/1‑65 (MQ=255) cGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACg > 1:5169788/1‑65 (MQ=255) atCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAAcc < 1:5623845/63‑2 (MQ=255) atCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAAcc < 1:4339776/63‑2 (MQ=255) atCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAAcc < 1:234300/63‑2 (MQ=255) ccTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAAcc < 1:3715333/62‑2 (MQ=255) cTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTca < 1:3961549/70‑2 (MQ=255) tGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGa > 1:5539454/1‑70 (MQ=255) gggTAACGAAAGACGCTTTACCCATAATACGACGGGtcat < 1:5414647/40‑1 (MQ=255) ggTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCa < 1:5377813/71‑1 (MQ=255) tAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGc > 1:4153184/1‑49 (MQ=255) tAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGc > 1:5176971/1‑49 (MQ=255) aaCGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAgcg > 1:4978297/1‑71 (MQ=255) cGAAAGACGCTTTACCCATAATACGACGGGTCATCATGc > 1:3304856/1‑39 (MQ=255) cGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCaa > 1:1099062/1‑48 (MQ=255) cGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCaa > 1:810261/1‑48 (MQ=255) gAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCt > 1:2343918/1‑70 (MQ=255) aaaGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTc > 1:3316697/1‑71 (MQ=255) aaGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTcc < 1:2878285/71‑1 (MQ=255) aggCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGt < 1:4023622/69‑1 (MQ=255) ggCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGtt > 1:4066856/2‑71 (MQ=255) gCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGttt < 1:2252670/57‑2 (MQ=255) gCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttc > 1:2613270/1‑71 (MQ=255) tttCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttcct > 1:3144320/1‑69 (MQ=255) tttACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGtt < 1:1295208/54‑1 (MQ=255) tACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttcttc < 1:2759822/70‑1 (MQ=255) aCCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCg < 1:776813/70‑1 (MQ=255) | GGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCG > Exported/1934130‑1934269 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |