| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,934,224 | G→T | 32.7% | R73R (CGC→CGA) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,934,224 | 0 | G | T | 32.7% | 164.7 / 75.6 | 104 | R73R (CGC→CGA) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (39/31); new base T (20/14); total (59/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
Only 100 of 106 total aligned reads displayed.
TGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGT > Exported/1934156‑1934294 | tGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCg > 1:65613/1‑71 (MQ=255) gAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCgg > 1:2526588/1‑71 (MQ=255) gAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCgg > 1:2287907/1‑71 (MQ=255) gAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCg > 1:1549295/1‑70 (MQ=255) gAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCg < 1:3245899/70‑1 (MQ=255) aaTACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCgg < 1:4012404/70‑1 (MQ=255) aaTACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCg < 1:4738092/69‑1 (MQ=255) cGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAg > 1:4926516/1‑70 (MQ=255) cGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAg > 1:1058439/1‑70 (MQ=255) cGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAg > 1:3759086/1‑70 (MQ=255) gCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCaa > 1:4279799/1‑68 (MQ=255) gCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCaa > 1:932022/1‑68 (MQ=255) gCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCaa > 1:5591560/1‑68 (MQ=255) ccAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACgg > 1:1209557/1‑70 (MQ=255) ccAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGc < 1:587097/71‑1 (MQ=255) aaCGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGc < 1:3508765/63‑1 (MQ=255) cGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACg > 1:5157556/1‑65 (MQ=255) cGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACg > 1:5169788/1‑65 (MQ=255) atCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAAcc < 1:234300/63‑2 (MQ=255) atCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAAcc < 1:4339776/63‑2 (MQ=255) atCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAAcc < 1:5623845/63‑2 (MQ=255) ccTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAAcc < 1:3715333/62‑2 (MQ=255) cTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTca < 1:3961549/70‑2 (MQ=255) tGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGa > 1:5539454/1‑70 (MQ=255) ggTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCa < 1:5377813/71‑1 (MQ=255) tAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGc > 1:5176971/1‑49 (MQ=255) tAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGc > 1:4153184/1‑49 (MQ=255) aaCGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAgcg > 1:4978297/1‑71 (MQ=255) cGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCaa > 1:1099062/1‑48 (MQ=255) cGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCaa > 1:810261/1‑48 (MQ=255) aaaGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTc > 1:3316697/1‑71 (MQ=255) aaGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTcc < 1:2878285/71‑1 (MQ=255) aggCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGt < 1:4023622/69‑1 (MQ=255) ggCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGtt > 1:4066856/2‑71 (MQ=255) gCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGttt < 1:2252670/57‑2 (MQ=255) gCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttc > 1:2613270/1‑71 (MQ=255) tttCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttcct > 1:3144320/1‑69 (MQ=255) tttACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGtt < 1:1295208/54‑1 (MQ=255) tACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttcttc < 1:2759822/70‑1 (MQ=255) aCCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCg < 1:776813/70‑1 (MQ=255) cccATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGttta > 1:3140687/1‑50 (MQ=255) cccATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGttta > 1:5115961/1‑50 (MQ=255) cccATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGttta > 1:3147290/1‑50 (MQ=255) cccATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGtt < 1:4873818/71‑1 (MQ=255) ccATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGttt > 1:4348514/1‑49 (MQ=255) ccATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGttt > 1:4023336/1‑49 (MQ=255) ccATAATACGACGGGTCATCATGCGGCCAGCAACGGCGa > 1:3181525/1‑39 (MQ=255) cATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGt < 1:903255/47‑1 (MQ=255) cATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCtt > 1:4280273/1‑56 (MQ=255) cATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTtct < 1:321230/71‑1 (MQ=255) cATAATACGACGGGTCATCATGCGGCCAGCAACCGCGACTTCGATGt < 1:4577549/47‑1 (MQ=255) taataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTc < 1:2739660/55‑1 (MQ=255) taataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTc < 1:2634463/55‑1 (MQ=255) taataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTc < 1:2608632/55‑1 (MQ=255) taataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGtt < 1:1588650/67‑1 (MQ=255) taataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTctct > 1:2161738/1‑71 (MQ=255) aataCGACGGGTCATCATGCGGCCAGCAACGGCGa > 1:2908312/1‑35 (MQ=255) aataCGACGGGTCATCATGCGGCCAGCAACGGCGa > 1:5406354/1‑35 (MQ=255) aataCGACGGGTCATCATGCGGCCAGCAACGGCGa > 1:699434/1‑35 (MQ=255) aataCGACGGGTCATCATGCGGCCAGCAACGGCGa > 1:2969353/1‑35 (MQ=255) aataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCtt > 1:386593/1‑71 (MQ=255) aataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCtt > 1:740975/1‑71 (MQ=255) ataCGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCctt > 1:435370/1‑71 (MQ=255) ataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGa > 1:1455992/1‑40 (MQ=255) ataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCttt > 1:5287180/1‑71 (MQ=255) ataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCttt < 1:846781/71‑1 (MQ=255) ataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCttt > 1:2285625/1‑71 (MQ=255) ataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCttt > 1:3534875/1‑71 (MQ=255) taCGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCcttc > 1:5462633/1‑70 (MQ=255) taCGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCcttc > 1:666016/1‑70 (MQ=255) cgacgGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCa < 1:5205759/52‑1 (MQ=255) cgacgGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGcc < 1:2983383/71‑1 (MQ=255) cgacgGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGc < 1:4796101/70‑1 (MQ=255) cgacgGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGc < 1:3307086/70‑1 (MQ=255) gggTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCg < 1:3866528/71‑1 (MQ=255) gTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGaa < 1:1442612/71‑1 (MQ=255) catcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttctt < 1:1445814/50‑1 (MQ=255) catcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAAt > 1:618336/1‑70 (MQ=255) atcatTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGt < 1:4439579/45‑1 (MQ=255) atcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAAtt < 1:686803/70‑1 (MQ=255) tcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTc < 1:2284141/40‑1 (MQ=255) tcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTc < 1:3931232/64‑1 (MQ=255) tcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCt < 1:4701572/71‑1 (MQ=255) taatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCttt > 1:5677460/3‑58 (MQ=255) catTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttcc < 1:4904945/46‑2 (MQ=255) catTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttcc < 1:5380309/46‑2 (MQ=255) catTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttc > 1:1633243/1‑45 (MQ=255) catTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttc > 1:4986892/1‑45 (MQ=255) catTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttc > 1:4980481/1‑45 (MQ=255) catGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGa < 1:4416385/65‑1 (MQ=255) catGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTg > 1:4585570/1‑71 (MQ=255) atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCg > 1:5287455/1‑48 (MQ=255) atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGtt > 1:5369954/1‑50 (MQ=255) atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTg < 1:4031458/70‑1 (MQ=255) atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTg < 1:923928/70‑1 (MQ=255) atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGc > 1:2425460/1‑71 (MQ=255) atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCCTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTg < 1:4652666/70‑1 (MQ=255) atGAGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCg > 1:4521076/1‑48 (MQ=255) gCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGt > 1:4291881/1‑71 (MQ=255) gCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGt > 1:17456/1‑71 (MQ=255) | TGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGT > Exported/1934156‑1934294 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |