| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,934,314 | A→G | 5.4% | D43D (GAT→GAC) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,934,314 | 0 | A | G | 5.4% | 271.5 / 3.9 | 93 | D43D (GAT→GAC) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base A (44/44); new base G (2/3); total (46/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
Only 100 of 103 total aligned reads displayed.
CGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTT > Exported/1934244‑1934384 | cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGa > 1:2747049/1‑71 (MQ=255)cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGa > 1:874971/1‑71 (MQ=255)cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGa > 1:3159777/1‑71 (MQ=255) gATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGa < 1:2304240/70‑1 (MQ=255) aTGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATc < 1:813340/71‑1 (MQ=255) ttCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATcgc < 1:3862576/70‑1 (MQ=255) cAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGAc > 1:5045988/1‑71 (MQ=255) aGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGAc < 1:4588395/70‑1 (MQ=255) cgcTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACgg > 1:527619/1‑70 (MQ=255) ttCCAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGGTCAGAGGTATGGTCGCGGCGAAAAt < 1:1887922/71‑1 (MQ=255) ttCCAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGGTCAGAGGTATGGTCGCGGCGAAAAt < 1:1378233/71‑1 (MQ=255) ccAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCg > 1:5144277/1‑71 (MQ=255) cAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATcgc < 1:3077494/59‑1 (MQ=255) cAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCg < 1:4192689/70‑1 (MQ=255) cAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGGTCAGAGGTATGGTCGCGGCGAAAATcat < 1:2879435/71‑3 (MQ=255) aGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGtt < 1:4859584/71‑1 (MQ=255) aGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGtt < 1:782698/71‑1 (MQ=255) tcttcGTTCTCTTTTCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTc > 1:4883809/1‑69 (MQ=255) cttcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGaaa > 1:5671097/1‑62 (MQ=255) cttcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATAATCGCGACGGAAATc > 1:1217364/1‑64 (MQ=255) cttcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGAATGATCGCGACGGAAATCGTTCgg > 1:3036027/1‑70 (MQ=255) tcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCggg > 1:1789374/1‑69 (MQ=255) cGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATcgc > 1:794405/1‑51 (MQ=255) cGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCg > 1:4170623/1‑66 (MQ=255) ctctTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGa > 1:5509245/1‑53 (MQ=255) ctctTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCa < 1:291340/70‑1 (MQ=255) tttGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGTGAAGGCaa < 1:4135763/68‑1 (MQ=255) ttGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATccc > 1:2208465/1‑71 (MQ=255) ttGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATccc > 1:135023/1‑71 (MQ=255) tGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATccc < 1:1579873/70‑1 (MQ=255) tGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATccc < 1:2585900/70‑1 (MQ=255) gCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAAt > 1:1095821/1‑50 (MQ=255) gCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAAt > 1:3991350/1‑50 (MQ=255) ccGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATcccc < 1:4026676/69‑1 (MQ=255) cGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGc < 1:1843521/71‑1 (MQ=255) gTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGTAAGGCa < 1:2540706/61‑1 (MQ=255) gAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGaa > 1:656993/1‑54 (MQ=255) gAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTcgc > 1:1808493/1‑71 (MQ=255) gAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTcgc > 1:64349/1‑71 (MQ=255) gAATTCTGCGTGCAATTGGTCAGAGGTATGATCGAGACGGAAAt < 1:2058055/44‑1 (MQ=255) aaTTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTcg < 1:4147397/69‑1 (MQ=255) actCTTCGTGCAACTGGTCAGAGGTATGGTCGCGGCGaaaa > 1:4392497/3‑41 (MQ=16) aTTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCg > 1:3744793/1‑44 (MQ=255) aTTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCg > 1:2498816/1‑44 (MQ=255) aTTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCg > 1:4325171/1‑44 (MQ=255) aTTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCg < 1:4652458/48‑1 (MQ=255) aTTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCg < 1:3401573/48‑1 (MQ=255) ttCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTc < 1:5139258/46‑1 (MQ=255) ttCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTc < 1:491613/46‑1 (MQ=255) ttCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTc < 1:3788408/46‑1 (MQ=255) ttCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTc < 1:2522063/46‑1 (MQ=255) ttCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAgg > 1:241232/1‑53 (MQ=255) ttCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCa < 1:2216551/55‑1 (MQ=255) ttCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATc < 1:4834194/58‑1 (MQ=255) ttCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATc < 1:3378175/58‑1 (MQ=255) ttCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATc < 1:2454716/58‑1 (MQ=255) ttCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCa < 1:4384224/71‑1 (MQ=255) ttCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCa < 1:108725/71‑1 (MQ=255) ttCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCa < 1:5286764/71‑1 (MQ=255) tCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCa < 1:1903398/70‑1 (MQ=255) cTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGaa > 1:1619200/1‑49 (MQ=255) cTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAg > 1:5444646/1‑50 (MQ=255) cTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCaa > 1:311872/1‑54 (MQ=255) cTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAAt > 1:3324164/1‑55 (MQ=255) cTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGc > 1:5496737/1‑62 (MQ=255) cTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGc > 1:2527226/1‑62 (MQ=255) cGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGt < 1:2409927/69‑1 (MQ=255) cGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTc < 1:662345/71‑1 (MQ=255) cGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTc < 1:528277/71‑1 (MQ=255) gTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCg < 1:4036320/71‑1 (MQ=255) tGCAACTGGTCAGAGGTATGGTCGCGGCGAAAATCATTGGGaaa > 1:2963896/1‑44 (MQ=12) cAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGaa > 1:5491482/1‑42 (MQ=255) cAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATc < 1:4665321/49‑1 (MQ=255) cAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGc > 1:5260985/1‑69 (MQ=255) aaTTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATcc < 1:1782765/49‑1 (MQ=255) aTTGGTCAGAGGTATGATCGCGACGGAAATCGTTCgg > 1:3563371/1‑37 (MQ=255) aTTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGc < 1:91511/71‑1 (MQ=255) ttGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCt > 1:5260953/1‑71 (MQ=255) ttGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCt > 1:413709/1‑71 (MQ=255) tGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCtt > 1:4444204/1‑71 (MQ=255) ggTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCtac > 1:2753793/1‑69 (MQ=255) gTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTtct < 1:2965615/71‑1 (MQ=255) gTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTtct < 1:2020225/71‑1 (MQ=255) tCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTtct > 1:3261035/1‑70 (MQ=255) agagGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATc < 1:785421/40‑1 (MQ=255) agagGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGc < 1:1557410/60‑1 (MQ=255) agGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCa > 1:4400948/1‑60 (MQ=255) agGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCa > 1:3639868/1‑60 (MQ=255) agGTATGATAGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCa > 1:1770200/1‑60 (MQ=255) tATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCac < 1:4902341/66‑1 (MQ=255) aTGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTtct > 1:1352544/1‑62 (MQ=255) aTGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCacga > 1:1962254/1‑67 (MQ=255) tGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTcgcg < 1:4681323/44‑1 (MQ=255) tGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGt > 1:1542334/1‑71 (MQ=255) aTCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTc > 1:3533288/1‑49 (MQ=255) aTCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCa < 1:2179405/53‑1 (MQ=255) aTCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGttt > 1:2547250/1‑71 (MQ=255) aTCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGtt < 1:1786343/70‑1 (MQ=255) aTCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGtt < 1:485836/70‑1 (MQ=255) aTCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGtt < 1:4873301/70‑1 (MQ=255) | CGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGGGAAGGCAATCCCCTGCTCGCGCAGGTTCGCCAGCTTCTCACGACGCGTTT > Exported/1934244‑1934384 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |